Comparison of antimicrobial effects of titanium tetrafluoride, chlorhexidine, xylitol and sodium fluoride on streptococcus mutans: An in-vitro study
Introduction: No studies have yet documented the bactericidal effects of TiF4, and its role in the treatment of dental caries, and no definite protocol has been introduced to regulate its use. The aim of this study was to determine the antimicrobial/bactericidal effects of TiF4 on Streptococcus Mu...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Electronic Physician
2017-03-01
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Series: | Electronic Physician |
Subjects: | |
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5407241/ |
Summary: | Introduction: No studies have yet documented the bactericidal effects of TiF4, and its role in the treatment of
dental caries, and no definite protocol has been introduced to regulate its use. The aim of this study was to
determine the antimicrobial/bactericidal effects of TiF4 on Streptococcus Mutans (S. Mutans) and to compare it
with chlorhexidine (Chx), sodium fluoride (NaF) and xylitol.
Methods: This study was conducted at the Shiraz University of Medical Sciences microbiology laboratory during
March 2015 to September 2015. In this in-vitro study, first a bacterial suspension was prepared and adjusted to a
0.5 McFarland standard (equivalent to 1×108 CFU/ml). The minimal inhibitory concentration (MIC) and minimal
bactericidal concentrations (MBC) of TiF4, Chx, NaF and xylitol were assessed using broth microdilution assay
and disk diffusion methods. In order to neutralize the acidic nature of TiF4, we used a sodium hydroxide
preparation to obtain a pH of 7.2 and repeated all of the previous tests with the neutralized TiF4 solution. We
reported the final results as percentages where appropriate.
Results: The MIC of TiF4, NaF and Chx for S. Mutans were 12.5%, 12.5% and 6.25%, respectively. At a
concentration of 12.5% the inhibition zone diameters were 9 mm, 15mm and 14mm for TiF4, NaF and Chx,
respectively. The MBC was 25%, 12.5% and 12.5% for TiF4, NaF and Chx, respectively. Xylitol failed to show
any bactericidal or growth inhibitory effect in all of its concentrations. When we repeated the tests with an
adjusted pH, identical results were obtained.
Conclusion: TiF4 solutions have anti-growth and bactericidal effects on S. Mutans at a concentration of 12.5%
which is comparable with chlorhexidine and NaF, indicating the possible use of this solution in dental practice as
an anti-cariogenic agent, furthermore the antimicrobial activity is unaffected by pH of the environment. |
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ISSN: | 2008-5842 2008-5842 |