Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana
Photosynthesis involves the conversion of sunlight energy into stored chemical energy, which is achieved through electron transport along a series of redox reactions. Excess photosynthetic electron transport might be dangerous due to the risk of molecular oxygen reduction, generating reactive oxygen...
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doaj-262c9c62670141a3aae58a1e01bedd432020-11-24T21:34:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-07-011010.3389/fpls.2019.00916459034Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thalianaYugo Lima-Melo0Yugo Lima-Melo1Vicente T. C. B. Alencar2Ana K. M. Lobo3Rachel H. V. Sousa4Mikko Tikkanen5Eva-Mari Aro6Joaquim A. G. Silveira7Peter J. Gollan8Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, BrazilMolecular Plant Biology, Department of Biochemistry, University of Turku, Turku, FinlandDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, BrazilMolecular Plant Biology, Department of Biochemistry, University of Turku, Turku, FinlandMolecular Plant Biology, Department of Biochemistry, University of Turku, Turku, FinlandDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, BrazilMolecular Plant Biology, Department of Biochemistry, University of Turku, Turku, FinlandPhotosynthesis involves the conversion of sunlight energy into stored chemical energy, which is achieved through electron transport along a series of redox reactions. Excess photosynthetic electron transport might be dangerous due to the risk of molecular oxygen reduction, generating reactive oxygen species (ROS) over-accumulation. Avoiding excess ROS production requires the rate of electron transport to be coordinated with the capacity of electron acceptors in the chloroplast stroma. Imbalance between the donor and acceptor sides of photosystem I (PSI) can lead to inactivation, which is called PSI photoinhibition. We used a light-inducible PSI photoinhibition system in Arabidopsis thaliana to resolve the time dynamics of inhibition and to investigate its impact on ROS production and turnover. The oxidation state of the PSI reaction center and rates of CO2 fixation both indicated strong and rapid PSI photoinhibition upon donor side/acceptor side imbalance, while the rate of inhibition eased during prolonged imbalance. PSI photoinhibition was not associated with any major changes in ROS accumulation or antioxidant activity; however, a lower level of lipid oxidation correlated with lower abundance of chloroplast lipoxygenase in PSI-inhibited leaves. The results of this study suggest that rapid activation of PSI photoinhibition under severe photosynthetic imbalance protects the chloroplast from over-reduction and excess ROS formation.https://www.frontiersin.org/article/10.3389/fpls.2019.00916/fullphotosystem IphotosynthesisROSCO2 fixationphotoinhibitionP700 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yugo Lima-Melo Yugo Lima-Melo Vicente T. C. B. Alencar Ana K. M. Lobo Rachel H. V. Sousa Mikko Tikkanen Eva-Mari Aro Joaquim A. G. Silveira Peter J. Gollan |
spellingShingle |
Yugo Lima-Melo Yugo Lima-Melo Vicente T. C. B. Alencar Ana K. M. Lobo Rachel H. V. Sousa Mikko Tikkanen Eva-Mari Aro Joaquim A. G. Silveira Peter J. Gollan Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana Frontiers in Plant Science photosystem I photosynthesis ROS CO2 fixation photoinhibition P700 |
author_facet |
Yugo Lima-Melo Yugo Lima-Melo Vicente T. C. B. Alencar Ana K. M. Lobo Rachel H. V. Sousa Mikko Tikkanen Eva-Mari Aro Joaquim A. G. Silveira Peter J. Gollan |
author_sort |
Yugo Lima-Melo |
title |
Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana |
title_short |
Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana |
title_full |
Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana |
title_fullStr |
Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana |
title_full_unstemmed |
Photoinhibition of Photosystem I Provides Oxidative Protection During Imbalanced Photosynthetic Electron Transport in Arabidopsis thaliana |
title_sort |
photoinhibition of photosystem i provides oxidative protection during imbalanced photosynthetic electron transport in arabidopsis thaliana |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2019-07-01 |
description |
Photosynthesis involves the conversion of sunlight energy into stored chemical energy, which is achieved through electron transport along a series of redox reactions. Excess photosynthetic electron transport might be dangerous due to the risk of molecular oxygen reduction, generating reactive oxygen species (ROS) over-accumulation. Avoiding excess ROS production requires the rate of electron transport to be coordinated with the capacity of electron acceptors in the chloroplast stroma. Imbalance between the donor and acceptor sides of photosystem I (PSI) can lead to inactivation, which is called PSI photoinhibition. We used a light-inducible PSI photoinhibition system in Arabidopsis thaliana to resolve the time dynamics of inhibition and to investigate its impact on ROS production and turnover. The oxidation state of the PSI reaction center and rates of CO2 fixation both indicated strong and rapid PSI photoinhibition upon donor side/acceptor side imbalance, while the rate of inhibition eased during prolonged imbalance. PSI photoinhibition was not associated with any major changes in ROS accumulation or antioxidant activity; however, a lower level of lipid oxidation correlated with lower abundance of chloroplast lipoxygenase in PSI-inhibited leaves. The results of this study suggest that rapid activation of PSI photoinhibition under severe photosynthetic imbalance protects the chloroplast from over-reduction and excess ROS formation. |
topic |
photosystem I photosynthesis ROS CO2 fixation photoinhibition P700 |
url |
https://www.frontiersin.org/article/10.3389/fpls.2019.00916/full |
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