Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.
BACKGROUND: MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regene...
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doaj-262d7f8e7340484a907675822b0d1b512020-11-24T21:26:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e2023810.1371/journal.pone.0020238Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation.Huan ChenYimin SunRuiqi DongShengsheng YangChuanyong PanDao XiangMingyong MiaoBinghua JiaoBACKGROUND: MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination. METHODOLOGY/PRINCIPAL FINDING: MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin βB (INHBB) and Met. In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver. CONCLUSION/SIGNIFICANCE: MiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34a-mediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation.http://europepmc.org/articles/PMC3105003?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huan Chen Yimin Sun Ruiqi Dong Shengsheng Yang Chuanyong Pan Dao Xiang Mingyong Miao Binghua Jiao |
spellingShingle |
Huan Chen Yimin Sun Ruiqi Dong Shengsheng Yang Chuanyong Pan Dao Xiang Mingyong Miao Binghua Jiao Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation. PLoS ONE |
author_facet |
Huan Chen Yimin Sun Ruiqi Dong Shengsheng Yang Chuanyong Pan Dao Xiang Mingyong Miao Binghua Jiao |
author_sort |
Huan Chen |
title |
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation. |
title_short |
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation. |
title_full |
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation. |
title_fullStr |
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation. |
title_full_unstemmed |
Mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation. |
title_sort |
mir-34a is upregulated during liver regeneration in rats and is associated with the suppression of hepatocyte proliferation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
BACKGROUND: MicroRNAs are a class of small regulatory RNAs that modulate a variety of biological processes, including cellular differentiation, apoptosis, metabolism and proliferation. This study aims to explore the effect of miR-34a in hepatocyte proliferation and its potential role in liver regeneration termination. METHODOLOGY/PRINCIPAL FINDING: MiR-34a was highly induced after partial hepatectomy. Overexpression of miR-34a in BRL-3A cells could significantly inhibit cell proliferation and down-regulate the expression of inhibin βB (INHBB) and Met. In BRL-3A cells, INHBB was identified as a direct target of miR-34a by luciferase reporter assay. More importantly, INHBB siRNA significantly repressed cell proliferation. A decrease of INHBB and Met was detected in regenerating liver. CONCLUSION/SIGNIFICANCE: MiR-34a expression was upregulated during the late phase of liver regeneration. MiR-34a-mediated regulation of INHBB and Met may collectively contribute to the suppression of hepatocyte proliferation. |
url |
http://europepmc.org/articles/PMC3105003?pdf=render |
work_keys_str_mv |
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