Vitamin D3 influence the Th1/Th2 ratio in C57BL/6 induced model of experimental autoimmune encephalomyelitis

• Main Authors: Maryam Soleimani, Seyed Behnamedin Jameie, Mehdi Mehdizadeh, Mahdieh Keradi, Masoumeh Masoumipoor, Soraya Mehrabi
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2014-10-01
• Series: Iranian Journal of Basic Medical Sciences
• Subjects: EAE Th1/Th2 ratio Vitamin D3
• Online Access: http://ijbms.mums.ac.ir/pdf_3454_eec666f551c2d4f6fa02bba41e7105c8.html

Objective(s): Multiple Sclerosis (MS) is known as a progressive inflammatory CNS disease. Cytokines belong to Th1 or Th2 family and inflammatory cells, play significant role in pathophysiology of MS. Thus, any treatment supposed to influence the relation between Th1 to Th2 cytokines expression. Although vitamin D has been prescribed as a therapeutic supplement of MS for a long time, it is not clear how much it may affect the Th1/Th2 ratio. To answer this question the present research was designed.  Materials and Methods: Thirty C57BL/6 adult female mice were used. The animals were randomly divided into trial and control groups. Experimental Autoimmune Encephalomyelitis (EAE) modeling for MS and clinical scoring as cited by others was used. Based on scoring and step of the disease vitamin D3 prescription (5 mg/kg) started and continued for three weeks. Results: By using ELISA and RT-PCR the brain level of TNF-α, IL-10, IL-4 and IL-12 determined. Significant decrease of clinical symptoms in trial group which received vitamin D was seen comparing to control animals (P