Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia
Data on genotypic diversity of Mycobacterium tuberculosis complex (MTBC) is important to understand its epidemiology, human adaptation, clinical phenotypes, and drug resistance. This study aimed to characterize MTBC clinical isolates circulating in a predominantly pastoralist are...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2014-01-01
|
Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2014/238532 |
id |
doaj-264191483d7d468eaee8ed964d48d48d |
---|---|
record_format |
Article |
spelling |
doaj-264191483d7d468eaee8ed964d48d48d2020-11-25T00:11:36ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/238532238532Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of EthiopiaMulugeta Belay0Gobena Ameni1Gunnar Bjune2David Couvin3Nalin Rastogi4Fekadu Abebe5Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, EthiopiaAklilu Lemma Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, EthiopiaSection for International Health, Department of Community Medicine, Institute for Health and Society, University of Oslo, P.O. Box 1130, Blindern, 0318 Oslo, NorwayWHO Supranational TB Reference Laboratory, TB & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, 97183 Abymes, FranceWHO Supranational TB Reference Laboratory, TB & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, 97183 Abymes, FranceSection for International Health, Department of Community Medicine, Institute for Health and Society, University of Oslo, P.O. Box 1130, Blindern, 0318 Oslo, NorwayData on genotypic diversity of Mycobacterium tuberculosis complex (MTBC) is important to understand its epidemiology, human adaptation, clinical phenotypes, and drug resistance. This study aimed to characterize MTBC clinical isolates circulating in a predominantly pastoralist area in Ethiopia, a country where tuberculosis is the second leading cause of mortality. Culture of sputum samples collected from a total of 325 pulmonary TB suspects was done to isolate MTBC. Spoligotyping was used to characterize 105 isolates from culture positive slopes and the result was compared with an international database. Forty-four spoligotype patterns were observed to correspond to 35 shared-types (SITs) containing 96 isolates and 9 orphan patterns; 27 SITs containing 83 isolates matched a preexisting shared-type in the database, whereas 8 SITs (n=13 isolates) were newly created. A total of 19 SITs containing 80 isolates were clustered within this study (overall clustering of 76.19%). Three dominant lineages (T, CAS, and Manu) accounted for 76.19% of the isolates. SIT149/T3-ETH was one of the two most dominant sublineages. Unlike previous reports, we show that Manu lineage strains not only constitute a dominant lineage, but are also associated with HIV infection in Afar region of Ethiopia. The high level of clustering suggests the presence of recent transmission that should be further studied using additional genotyping markers.http://dx.doi.org/10.1155/2014/238532 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mulugeta Belay Gobena Ameni Gunnar Bjune David Couvin Nalin Rastogi Fekadu Abebe |
spellingShingle |
Mulugeta Belay Gobena Ameni Gunnar Bjune David Couvin Nalin Rastogi Fekadu Abebe Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia BioMed Research International |
author_facet |
Mulugeta Belay Gobena Ameni Gunnar Bjune David Couvin Nalin Rastogi Fekadu Abebe |
author_sort |
Mulugeta Belay |
title |
Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia |
title_short |
Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia |
title_full |
Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia |
title_fullStr |
Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia |
title_full_unstemmed |
Strain Diversity of Mycobacterium tuberculosis Isolates from Pulmonary Tuberculosis Patients in Afar Pastoral Region of Ethiopia |
title_sort |
strain diversity of mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in afar pastoral region of ethiopia |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
Data on genotypic diversity of Mycobacterium tuberculosis complex (MTBC) is important to understand its epidemiology, human adaptation, clinical phenotypes, and drug resistance.
This study aimed to characterize MTBC clinical isolates circulating in a predominantly pastoralist area in Ethiopia, a country where tuberculosis is the second leading cause of mortality.
Culture of sputum samples collected from a total of 325 pulmonary TB suspects was done to isolate MTBC. Spoligotyping was used to characterize 105 isolates from culture positive
slopes and the result was compared with an international database. Forty-four spoligotype patterns were observed to correspond to 35 shared-types (SITs) containing 96 isolates and
9 orphan patterns; 27 SITs containing 83 isolates matched a preexisting shared-type in the database, whereas 8 SITs (n=13 isolates) were newly created. A total of 19 SITs containing
80 isolates were clustered within this study (overall clustering of 76.19%). Three dominant lineages (T, CAS, and Manu) accounted for 76.19% of the isolates. SIT149/T3-ETH was one
of the two most dominant sublineages. Unlike previous reports, we show that Manu lineage strains not only constitute a dominant lineage, but are also associated with HIV infection in
Afar region of Ethiopia. The high level of clustering suggests the presence of recent transmission that should be further studied using additional genotyping markers. |
url |
http://dx.doi.org/10.1155/2014/238532 |
work_keys_str_mv |
AT mulugetabelay straindiversityofmycobacteriumtuberculosisisolatesfrompulmonarytuberculosispatientsinafarpastoralregionofethiopia AT gobenaameni straindiversityofmycobacteriumtuberculosisisolatesfrompulmonarytuberculosispatientsinafarpastoralregionofethiopia AT gunnarbjune straindiversityofmycobacteriumtuberculosisisolatesfrompulmonarytuberculosispatientsinafarpastoralregionofethiopia AT davidcouvin straindiversityofmycobacteriumtuberculosisisolatesfrompulmonarytuberculosispatientsinafarpastoralregionofethiopia AT nalinrastogi straindiversityofmycobacteriumtuberculosisisolatesfrompulmonarytuberculosispatientsinafarpastoralregionofethiopia AT fekaduabebe straindiversityofmycobacteriumtuberculosisisolatesfrompulmonarytuberculosispatientsinafarpastoralregionofethiopia |
_version_ |
1725403228009398272 |