Novel peptide marker corresponding to salivary protein gSG6 potentially identifies exposure to Anopheles bites.
In order to improve malaria control, and under the aegis of WHO recommendations, many efforts are being devoted to developing new tools for identifying geographic areas with high risk of parasite transmission. Evaluation of the human antibody response to arthropod salivary proteins could be an epide...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2008-06-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC2427200?pdf=render |
id |
doaj-266188cc172d42f08d916dcaff3364ed |
---|---|
record_format |
Article |
spelling |
doaj-266188cc172d42f08d916dcaff3364ed2020-11-25T01:45:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-06-0136e247210.1371/journal.pone.0002472Novel peptide marker corresponding to salivary protein gSG6 potentially identifies exposure to Anopheles bites.Anne PoinsignonSylvie CornelieMontserrat Mestres-SimonAlessandra LanfrancottiMarie RossignolDenis BoulangerBadara CisseCheikh SokhnaBruno ArcàFrançois SimondonFranck RemoueIn order to improve malaria control, and under the aegis of WHO recommendations, many efforts are being devoted to developing new tools for identifying geographic areas with high risk of parasite transmission. Evaluation of the human antibody response to arthropod salivary proteins could be an epidemiological indicator of exposure to vector bites, and therefore to risk of pathogen transmission. In the case of malaria, which is transmitted only by anopheline mosquitoes, maximal specificity could be achieved through identification of immunogenic proteins specific to the Anopheles genus. The objective of the present study was to determine whether the IgG response to the Anopheles gambiae gSG6 protein, from its recombinant form to derived synthetic peptides, could be an immunological marker of exposure specific to Anopheles gambiae bites.Specific IgG antibodies to recombinant gSG6 protein were observed in children living in a Senegalese area exposed to malaria. With the objective of optimizing Anopheles specificity and reproducibility, we designed five gSG6-based peptide sequences using a bioinformatic approach, taking into consideration i) their potential antigenic properties and ii) the absence of cross-reactivity with protein sequences of other arthropods/organisms. The specific anti-peptide IgG antibody response was evaluated in exposed children. The five gSG6 peptides showed differing antigenic properties, with gSG6-P1 and gSG6-P2 exhibiting the highest antigenicity. However, a significant increase in the specific IgG response during the rainy season and a positive association between the IgG level and the level of exposure to Anopheles gambiae bites was significant only for gSG6-P1.This step-by-step approach suggests that gSG6-P1 could be an optimal candidate marker for evaluating exposure to Anopheles gambiae bites. This marker could be employed as a geographic indicator, like remote sensing techniques, for mapping the risk of malaria. It could also represent a direct criterion of efficacy in evaluation of vector control strategies.http://europepmc.org/articles/PMC2427200?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anne Poinsignon Sylvie Cornelie Montserrat Mestres-Simon Alessandra Lanfrancotti Marie Rossignol Denis Boulanger Badara Cisse Cheikh Sokhna Bruno Arcà François Simondon Franck Remoue |
spellingShingle |
Anne Poinsignon Sylvie Cornelie Montserrat Mestres-Simon Alessandra Lanfrancotti Marie Rossignol Denis Boulanger Badara Cisse Cheikh Sokhna Bruno Arcà François Simondon Franck Remoue Novel peptide marker corresponding to salivary protein gSG6 potentially identifies exposure to Anopheles bites. PLoS ONE |
author_facet |
Anne Poinsignon Sylvie Cornelie Montserrat Mestres-Simon Alessandra Lanfrancotti Marie Rossignol Denis Boulanger Badara Cisse Cheikh Sokhna Bruno Arcà François Simondon Franck Remoue |
author_sort |
Anne Poinsignon |
title |
Novel peptide marker corresponding to salivary protein gSG6 potentially identifies exposure to Anopheles bites. |
title_short |
Novel peptide marker corresponding to salivary protein gSG6 potentially identifies exposure to Anopheles bites. |
title_full |
Novel peptide marker corresponding to salivary protein gSG6 potentially identifies exposure to Anopheles bites. |
title_fullStr |
Novel peptide marker corresponding to salivary protein gSG6 potentially identifies exposure to Anopheles bites. |
title_full_unstemmed |
Novel peptide marker corresponding to salivary protein gSG6 potentially identifies exposure to Anopheles bites. |
title_sort |
novel peptide marker corresponding to salivary protein gsg6 potentially identifies exposure to anopheles bites. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2008-06-01 |
description |
In order to improve malaria control, and under the aegis of WHO recommendations, many efforts are being devoted to developing new tools for identifying geographic areas with high risk of parasite transmission. Evaluation of the human antibody response to arthropod salivary proteins could be an epidemiological indicator of exposure to vector bites, and therefore to risk of pathogen transmission. In the case of malaria, which is transmitted only by anopheline mosquitoes, maximal specificity could be achieved through identification of immunogenic proteins specific to the Anopheles genus. The objective of the present study was to determine whether the IgG response to the Anopheles gambiae gSG6 protein, from its recombinant form to derived synthetic peptides, could be an immunological marker of exposure specific to Anopheles gambiae bites.Specific IgG antibodies to recombinant gSG6 protein were observed in children living in a Senegalese area exposed to malaria. With the objective of optimizing Anopheles specificity and reproducibility, we designed five gSG6-based peptide sequences using a bioinformatic approach, taking into consideration i) their potential antigenic properties and ii) the absence of cross-reactivity with protein sequences of other arthropods/organisms. The specific anti-peptide IgG antibody response was evaluated in exposed children. The five gSG6 peptides showed differing antigenic properties, with gSG6-P1 and gSG6-P2 exhibiting the highest antigenicity. However, a significant increase in the specific IgG response during the rainy season and a positive association between the IgG level and the level of exposure to Anopheles gambiae bites was significant only for gSG6-P1.This step-by-step approach suggests that gSG6-P1 could be an optimal candidate marker for evaluating exposure to Anopheles gambiae bites. This marker could be employed as a geographic indicator, like remote sensing techniques, for mapping the risk of malaria. It could also represent a direct criterion of efficacy in evaluation of vector control strategies. |
url |
http://europepmc.org/articles/PMC2427200?pdf=render |
work_keys_str_mv |
AT annepoinsignon novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT sylviecornelie novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT montserratmestressimon novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT alessandralanfrancotti novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT marierossignol novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT denisboulanger novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT badaracisse novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT cheikhsokhna novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT brunoarca novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT francoissimondon novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites AT franckremoue novelpeptidemarkercorrespondingtosalivaryproteingsg6potentiallyidentifiesexposuretoanophelesbites |
_version_ |
1725021670683443200 |