Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease
The most sensitive cell culture system for the isolation of foot-and-mouth disease virus (FMDV) is primary bovine thyroid (BTY) cells. However, BTY cells are seldom used because of the challenges associated with sourcing thyroids from FMDV-negative calves (particularly in FMD endemic countries), and...
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doaj-267c9de673f34caa8d80df158e20e5b42020-11-25T03:49:26ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-07-01710.3389/fvets.2020.00426551802Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular DiseaseAshley R. GrayBritta A. WoodElisabeth HenryMehreen AzharDonald P. KingValérie MiouletThe most sensitive cell culture system for the isolation of foot-and-mouth disease virus (FMDV) is primary bovine thyroid (BTY) cells. However, BTY cells are seldom used because of the challenges associated with sourcing thyroids from FMDV-negative calves (particularly in FMD endemic countries), and the costs and time required to regularly prepare batches of cells. Two continuous cell lines, a fetal goat tongue cell line (ZZ-R 127) and a fetal porcine kidney cell line (LFBK-αVβ6), have been shown to be highly sensitive to FMDV. Here, we assessed the sensitivity of ZZ-R 127 and LFBK-αVβ6 cells relative to primary BTY cells by titrating a range of FMDV original samples and isolates. Both the ZZ-R 127 and LFBK-αVβ6 cells were susceptible to FMDV for >100 passages, and there were no significant differences in sensitivity relative to primary BTY cells. Notably, the LFBK-αVβ6 cell line was highly sensitive to the O/CATHAY porcine-adapted FMDV strain. These results support the use of ZZ-R 127 and LFBK-αVβ6 as sensitive alternatives to BTY cells for the isolation of FMDV, and highlight the use of LFBK-αVβ6 cells as an additional tool for the isolation of porcinophilic viruses.https://www.frontiersin.org/article/10.3389/fvets.2020.00426/fullfoot-and-mouth disease virusvirus isolationcell linediagnosisvesicular viruses |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ashley R. Gray Britta A. Wood Elisabeth Henry Mehreen Azhar Donald P. King Valérie Mioulet |
spellingShingle |
Ashley R. Gray Britta A. Wood Elisabeth Henry Mehreen Azhar Donald P. King Valérie Mioulet Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease Frontiers in Veterinary Science foot-and-mouth disease virus virus isolation cell line diagnosis vesicular viruses |
author_facet |
Ashley R. Gray Britta A. Wood Elisabeth Henry Mehreen Azhar Donald P. King Valérie Mioulet |
author_sort |
Ashley R. Gray |
title |
Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease |
title_short |
Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease |
title_full |
Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease |
title_fullStr |
Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease |
title_full_unstemmed |
Evaluation of Cell Lines for the Isolation of Foot-and-Mouth Disease Virus and Other Viruses Causing Vesicular Disease |
title_sort |
evaluation of cell lines for the isolation of foot-and-mouth disease virus and other viruses causing vesicular disease |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2020-07-01 |
description |
The most sensitive cell culture system for the isolation of foot-and-mouth disease virus (FMDV) is primary bovine thyroid (BTY) cells. However, BTY cells are seldom used because of the challenges associated with sourcing thyroids from FMDV-negative calves (particularly in FMD endemic countries), and the costs and time required to regularly prepare batches of cells. Two continuous cell lines, a fetal goat tongue cell line (ZZ-R 127) and a fetal porcine kidney cell line (LFBK-αVβ6), have been shown to be highly sensitive to FMDV. Here, we assessed the sensitivity of ZZ-R 127 and LFBK-αVβ6 cells relative to primary BTY cells by titrating a range of FMDV original samples and isolates. Both the ZZ-R 127 and LFBK-αVβ6 cells were susceptible to FMDV for >100 passages, and there were no significant differences in sensitivity relative to primary BTY cells. Notably, the LFBK-αVβ6 cell line was highly sensitive to the O/CATHAY porcine-adapted FMDV strain. These results support the use of ZZ-R 127 and LFBK-αVβ6 as sensitive alternatives to BTY cells for the isolation of FMDV, and highlight the use of LFBK-αVβ6 cells as an additional tool for the isolation of porcinophilic viruses. |
topic |
foot-and-mouth disease virus virus isolation cell line diagnosis vesicular viruses |
url |
https://www.frontiersin.org/article/10.3389/fvets.2020.00426/full |
work_keys_str_mv |
AT ashleyrgray evaluationofcelllinesfortheisolationoffootandmouthdiseasevirusandothervirusescausingvesiculardisease AT brittaawood evaluationofcelllinesfortheisolationoffootandmouthdiseasevirusandothervirusescausingvesiculardisease AT elisabethhenry evaluationofcelllinesfortheisolationoffootandmouthdiseasevirusandothervirusescausingvesiculardisease AT mehreenazhar evaluationofcelllinesfortheisolationoffootandmouthdiseasevirusandothervirusescausingvesiculardisease AT donaldpking evaluationofcelllinesfortheisolationoffootandmouthdiseasevirusandothervirusescausingvesiculardisease AT valeriemioulet evaluationofcelllinesfortheisolationoffootandmouthdiseasevirusandothervirusescausingvesiculardisease |
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