Purification of gangliosides by liquid-liquid partition chromatography
A liquid-liquid partition chromatographic technique was applied to separate amphiphilic glycolipids. A two-phase solvent system composed of n-butanol-t-butyl methyl ether-acetonitrile-water at a volume ratio of 3:1:1:5 was found to be suitable for separating the gangliosides from total lipids extrac...
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2008-11-01
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520346241 |
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doaj-268a360f17e54f508d2ada756c3cd30f2021-04-28T05:58:17ZengElsevierJournal of Lipid Research0022-22752008-11-01491124742478Purification of gangliosides by liquid-liquid partition chromatographyTomohisa Kato0Kenichi Hatanaka1Japan Chemical Innovation Institute, 1-3-5 Jimbocho, Chiyoda-ku, Tokyo 101-0051, Japan; Kaneka Corporation, 3-2-4, Nakanoshima, Kita-ku, Osaka 530-8288, JapanInstitute of Industrial Science, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8505, JapanA liquid-liquid partition chromatographic technique was applied to separate amphiphilic glycolipids. A two-phase solvent system composed of n-butanol-t-butyl methyl ether-acetonitrile-water at a volume ratio of 3:1:1:5 was found to be suitable for separating the gangliosides from total lipids extracted from rat brain by liquid-liquid partition chromatographic systems, namely centrifugal partition chromatography (CPC) and high-speed counter-current chromatography. GM1 could be separated rapidly by using the upper phase as stationary phase for both systems. Moreover, various kinds of gangliosides (GM1, GD1a, GD1b, GT1b) could be separated individually by using the lower phase as stationary phase by CPC. The sample can be recovered without loss by these systems.http://www.sciencedirect.com/science/article/pii/S0022227520346241gangliosideseparationcentrifugal partition chromatographcounter-current chromatography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tomohisa Kato Kenichi Hatanaka |
spellingShingle |
Tomohisa Kato Kenichi Hatanaka Purification of gangliosides by liquid-liquid partition chromatography Journal of Lipid Research ganglioside separation centrifugal partition chromatograph counter-current chromatography |
author_facet |
Tomohisa Kato Kenichi Hatanaka |
author_sort |
Tomohisa Kato |
title |
Purification of gangliosides by liquid-liquid partition chromatography |
title_short |
Purification of gangliosides by liquid-liquid partition chromatography |
title_full |
Purification of gangliosides by liquid-liquid partition chromatography |
title_fullStr |
Purification of gangliosides by liquid-liquid partition chromatography |
title_full_unstemmed |
Purification of gangliosides by liquid-liquid partition chromatography |
title_sort |
purification of gangliosides by liquid-liquid partition chromatography |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2008-11-01 |
description |
A liquid-liquid partition chromatographic technique was applied to separate amphiphilic glycolipids. A two-phase solvent system composed of n-butanol-t-butyl methyl ether-acetonitrile-water at a volume ratio of 3:1:1:5 was found to be suitable for separating the gangliosides from total lipids extracted from rat brain by liquid-liquid partition chromatographic systems, namely centrifugal partition chromatography (CPC) and high-speed counter-current chromatography. GM1 could be separated rapidly by using the upper phase as stationary phase for both systems. Moreover, various kinds of gangliosides (GM1, GD1a, GD1b, GT1b) could be separated individually by using the lower phase as stationary phase by CPC. The sample can be recovered without loss by these systems. |
topic |
ganglioside separation centrifugal partition chromatograph counter-current chromatography |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520346241 |
work_keys_str_mv |
AT tomohisakato purificationofgangliosidesbyliquidliquidpartitionchromatography AT kenichihatanaka purificationofgangliosidesbyliquidliquidpartitionchromatography |
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1721504809488482304 |