Data for chicken semen proteome and label free quantitative analyses displaying sperm quality biomarkers

• Main Authors: Valérie Labas, Isabelle Grasseau, Karine Cahier, Audrey Gargaros, Grégoire Harichaux, Ana-Paula Teixeira-Gomes, Sabine Alves, Marie Bourin, Nadine Gérard, Elisabeth Blesbois
• Format: Article
• Language: English
• Published: Elsevier 2014-12-01
• Series: Data in Brief
• Subjects: Chicken (Gallus gallus); Spermatozoa; Seminal plasma; Proteome; Fertility
• Online Access: http://www.sciencedirect.com/science/article/pii/S2352340914000122

Understanding of biology of the avian male gamete is essential to improve the conservation of genetic resources and performances in farming. In this study, the semen proteome of the main domestic avian species (Gallus gallus) and evaluation of the molecular phenotype related to sperm quality were investigated using GeLC–MS/MS approach and label-free quantitative proteomic based on Spectral Counting (SC) and extracted ion chromatograms (XIC) methods. Here we describe in details the peptide/protein inventory of chicken ejaculated spermatozoa (SPZ) and seminal plasma (SP). We also show differential analyses of chicken semen (SPZ and corresponding SP) from 11 males demonstrating different levels of fertilizing capacity and sperm motility. The interpretation and description of these data can be found in a research article published by Labas and colleagues in the Journal of Proteomics in 2014 [1]. This is a new resource for exploring the molecular mechanisms involved in fertilizing capacity and to reveal new sets of fertility biomarkers.