An Automated and Miniaturized Rotating-Disk Device for Rapid Nucleic Acid Extraction

• Main Authors: Rui Tong, Lijuan Zhang, Chuandeng Hu, Xuee Chen, Qi Song, Kai Lou, Xin Tang, Yongsheng Chen, Xiuqing Gong, Yibo Gao, Weijia Wen
• Format: Article
• Language: English
• Published: MDPI AG 2019-03-01
• Series: Micromachines
• Subjects: sample preparation; nucleic acid; DNA; RNA
• Online Access: https://www.mdpi.com/2072-666X/10/3/204

The result of molecular diagnostic and detection greatly dependent on the quality and integrity of the isolated nucleic acid. In this work, we developed an automated miniaturized nucleic acid extraction device based on magnetic beads method, consisting of four components including a sample processing disc and its associated rotary power output mechanism, a pipetting module, a magnet module and an external central controller to enable a customizable and automated robust nucleic acid sample preparation. The extracted nucleic acid using 293T cells were verified using real-time polymerase chain reaction (PCR) and the data implies a comparable efficiency to a manual process, with the advantages of performing a flexible, time-saving (~10 min), and simple nucleic acid sample preparation.