Effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of Lateolabrax japonicus
Condensed tannins (CT) have been observed to be beneficial to maintain glucose homeostasis and improve carbohydrate utilization for livestock, whereas little information is available about the effects of CT on glycometabolism of fish. Three diets were formulated to contain approximately 15 % carbohy...
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doaj-269b60ba5ca34d2aa6b4bac50711a7852020-11-25T03:56:00ZengElsevierAquaculture Reports2352-51342020-11-0118100515Effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of Lateolabrax japonicusKai Peng0Guoxia Wang1Hongxia Zhao2Yuxi Wang3Wenyan Mo4Haomin Wu5Yanhua Huang6Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangdong 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangdong 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangdong 510640, ChinaAgriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB, T1J 4B1, CanadaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangdong 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangdong 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangdong 510640, China; Corresponding author.Condensed tannins (CT) have been observed to be beneficial to maintain glucose homeostasis and improve carbohydrate utilization for livestock, whereas little information is available about the effects of CT on glycometabolism of fish. Three diets were formulated to contain approximately 15 % carbohydrate (G1), 39 % carbohydrate (G2), and G2 supplemented with 0.1 % CT. A total of 225 juvenile Lateolabrax japonicus were randomly assigned to 3 groups (triplicate tanks per group) with 25 fish in each tank and fed to apparent satiation during 56-d feeding trial. Results indicated that fish fed G2 had lower (p < 0.05) final body weight, weight gain rate, feed intake, specific growth rate and serum insulin concentration, but higher (p < 0.05) feed efficiency ratio and serum glucose, total antioxidant capacity, superoxide dismutase, immunoglobulin M and lipopolysaccharide than those fish fed G1 or G3. The activity of pyruvate kinase (PK) was lower (p < 0.05) but glucokinase (GK) was higher (p < 0.05) in G2 than in G1 or G3. Gene expression of insulin like growth factor Ⅰ and PK were down-regulated (p < 0.05), whereas GK was up-regulated (p < 0.05) in G2 than in G1 or G3. No difference (p> 0.05) in these gene expressions were observed between G1 and G3. It was concluded that L. japonicus fed diet with 39 % carbohydrate exhibited depressed growth performance, increased antioxidant and immune capacity, and hyperglycemia. Supplementation of 0.1 % CT resulted in improved growth performance and decreased serum glucose concentration, mainly due to the positive effects of CT on the activity and gene expression of hepatic glycolysis and gluconeogenesis enzymes of L. japonicus.http://www.sciencedirect.com/science/article/pii/S2352513420306050CarbohydrateCondensed tanninsGlycometabolismLateolabrax japonicus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kai Peng Guoxia Wang Hongxia Zhao Yuxi Wang Wenyan Mo Haomin Wu Yanhua Huang |
spellingShingle |
Kai Peng Guoxia Wang Hongxia Zhao Yuxi Wang Wenyan Mo Haomin Wu Yanhua Huang Effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of Lateolabrax japonicus Aquaculture Reports Carbohydrate Condensed tannins Glycometabolism Lateolabrax japonicus |
author_facet |
Kai Peng Guoxia Wang Hongxia Zhao Yuxi Wang Wenyan Mo Haomin Wu Yanhua Huang |
author_sort |
Kai Peng |
title |
Effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of Lateolabrax japonicus |
title_short |
Effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of Lateolabrax japonicus |
title_full |
Effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of Lateolabrax japonicus |
title_fullStr |
Effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of Lateolabrax japonicus |
title_full_unstemmed |
Effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of Lateolabrax japonicus |
title_sort |
effect of high level of carbohydrate and supplementation of condensed tannins on growth performance, serum metabolites, antioxidant and immune response, and hepatic glycometabolism gene expression of lateolabrax japonicus |
publisher |
Elsevier |
series |
Aquaculture Reports |
issn |
2352-5134 |
publishDate |
2020-11-01 |
description |
Condensed tannins (CT) have been observed to be beneficial to maintain glucose homeostasis and improve carbohydrate utilization for livestock, whereas little information is available about the effects of CT on glycometabolism of fish. Three diets were formulated to contain approximately 15 % carbohydrate (G1), 39 % carbohydrate (G2), and G2 supplemented with 0.1 % CT. A total of 225 juvenile Lateolabrax japonicus were randomly assigned to 3 groups (triplicate tanks per group) with 25 fish in each tank and fed to apparent satiation during 56-d feeding trial. Results indicated that fish fed G2 had lower (p < 0.05) final body weight, weight gain rate, feed intake, specific growth rate and serum insulin concentration, but higher (p < 0.05) feed efficiency ratio and serum glucose, total antioxidant capacity, superoxide dismutase, immunoglobulin M and lipopolysaccharide than those fish fed G1 or G3. The activity of pyruvate kinase (PK) was lower (p < 0.05) but glucokinase (GK) was higher (p < 0.05) in G2 than in G1 or G3. Gene expression of insulin like growth factor Ⅰ and PK were down-regulated (p < 0.05), whereas GK was up-regulated (p < 0.05) in G2 than in G1 or G3. No difference (p> 0.05) in these gene expressions were observed between G1 and G3. It was concluded that L. japonicus fed diet with 39 % carbohydrate exhibited depressed growth performance, increased antioxidant and immune capacity, and hyperglycemia. Supplementation of 0.1 % CT resulted in improved growth performance and decreased serum glucose concentration, mainly due to the positive effects of CT on the activity and gene expression of hepatic glycolysis and gluconeogenesis enzymes of L. japonicus. |
topic |
Carbohydrate Condensed tannins Glycometabolism Lateolabrax japonicus |
url |
http://www.sciencedirect.com/science/article/pii/S2352513420306050 |
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