P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules

A set of dual functional small molecules (DFSMs) containing different amino acids has been synthesized and employed together with three different variants of the cytochrome P450 monooxygenase P450BM3 from Bacillus megaterium in H2O2-de...

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Main Authors:	Sébastien J.-P. Willot, Florian Tieves, Marco Girhard, Vlada B. Urlacher, Frank Hollmann, Gonzalo de Gonzalo
Format:	Article
Language:	English
Published:	MDPI AG 2019-06-01
Series:	Catalysts
Subjects:	biocatalysis cytochrome P450 dual functional small molecules epoxidations sulfoxidation
Online Access:	https://www.mdpi.com/2073-4344/9/7/567

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spelling	doaj-26bd75e7cbb14b88b4d42641cf4a5eb32020-11-25T00:47:47ZengMDPI AGCatalysts2073-43442019-06-019756710.3390/catal9070567catal9070567P450BM3-Catalyzed Oxidations Employing Dual Functional Small MoleculesSébastien J.-P. Willot0Florian Tieves1Marco Girhard2Vlada B. Urlacher3Frank Hollmann4Gonzalo de Gonzalo5Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The NetherlandsInstitute of Biochemistry, Heinrich-Heine University Düsseldorf, Universitätsstraße 1, 40225 Düsseldorf, GermanyInstitute of Biochemistry, Heinrich-Heine University Düsseldorf, Universitätsstraße 1, 40225 Düsseldorf, GermanyDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The NetherlandsDepartamento de Química Orgánica, Universidad de Sevilla, c/ Profesor García González 1, 41012 Sevilla, SpainA set of dual functional small molecules (DFSMs) containing different amino acids has been synthesized and employed together with three different variants of the cytochrome P450 monooxygenase P450BM3 from <i>Bacillus megaterium</i> in H<sub>2</sub>O<sub>2</sub>-dependent oxidation reactions. These DFSMs enhance P450BM3 activity with hydrogen peroxide as an oxidant, converting these enzymes into formal peroxygenases. This system has been employed for the catalytic epoxidation of styrene and in the sulfoxidation of thioanisole. Various P450BM3 variants have been evaluated in terms of activity and selectivity of the peroxygenase reactions.https://www.mdpi.com/2073-4344/9/7/567biocatalysiscytochrome P450dual functional small moleculesepoxidationssulfoxidation
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sébastien J.-P. Willot Florian Tieves Marco Girhard Vlada B. Urlacher Frank Hollmann Gonzalo de Gonzalo
spellingShingle	Sébastien J.-P. Willot Florian Tieves Marco Girhard Vlada B. Urlacher Frank Hollmann Gonzalo de Gonzalo P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules Catalysts biocatalysis cytochrome P450 dual functional small molecules epoxidations sulfoxidation
author_facet	Sébastien J.-P. Willot Florian Tieves Marco Girhard Vlada B. Urlacher Frank Hollmann Gonzalo de Gonzalo
author_sort	Sébastien J.-P. Willot
title	P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules
title_short	P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules
title_full	P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules
title_fullStr	P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules
title_full_unstemmed	P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules
title_sort	p450bm3-catalyzed oxidations employing dual functional small molecules
publisher	MDPI AG
series	Catalysts
issn	2073-4344
publishDate	2019-06-01
description	A set of dual functional small molecules (DFSMs) containing different amino acids has been synthesized and employed together with three different variants of the cytochrome P450 monooxygenase P450BM3 from <i>Bacillus megaterium</i> in H<sub>2</sub>O<sub>2</sub>-dependent oxidation reactions. These DFSMs enhance P450BM3 activity with hydrogen peroxide as an oxidant, converting these enzymes into formal peroxygenases. This system has been employed for the catalytic epoxidation of styrene and in the sulfoxidation of thioanisole. Various P450BM3 variants have been evaluated in terms of activity and selectivity of the peroxygenase reactions.
topic	biocatalysis cytochrome P450 dual functional small molecules epoxidations sulfoxidation
url	https://www.mdpi.com/2073-4344/9/7/567
work_keys_str_mv	AT sebastienjpwillot p450bm3catalyzedoxidationsemployingdualfunctionalsmallmolecules AT floriantieves p450bm3catalyzedoxidationsemployingdualfunctionalsmallmolecules AT marcogirhard p450bm3catalyzedoxidationsemployingdualfunctionalsmallmolecules AT vladaburlacher p450bm3catalyzedoxidationsemployingdualfunctionalsmallmolecules AT frankhollmann p450bm3catalyzedoxidationsemployingdualfunctionalsmallmolecules AT gonzalodegonzalo p450bm3catalyzedoxidationsemployingdualfunctionalsmallmolecules
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P450BM3-Catalyzed Oxidations Employing Dual Functional Small Molecules

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