Set-up of a method using LC-UV to assay mometasone furoate in pharmaceutical dosage forms

Set-up of a method using LC-UV to assay mometasone furoate in pharmaceutical dosage forms

A reliable method using LC-UV to assay mometasone furoate (MF) in creams or nasal sprays using the same chromatographic conditions was set up. Methanol:water 80:20 (v/v) (1.0 mL min-1) was used as mobile phase. MF was detected at 248 nm and analyzed in a concentration range from 1.0 to 20.0 µg mL-1....

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Bibliographic Details
Main Authors: Aline Ferreira Ourique, Renata Vidor Contri, Silvia Stanisçuaski Guterres, Ruy Carlos Ruver Beck, Adriana Raffin Pohlmann, Ana Melero, Ulrich F. Schaefer
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2012-01-01
Series:Química Nova
Subjects:
pharmaceuticals
liquid chromatography
mometasone
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000400030&lng=en&tlng=en
Description
Summary:A reliable method using LC-UV to assay mometasone furoate (MF) in creams or nasal sprays using the same chromatographic conditions was set up. Methanol:water 80:20 (v/v) (1.0 mL min-1) was used as mobile phase. MF was detected at 248 nm and analyzed in a concentration range from 1.0 to 20.0 µg mL-1. The method provided acceptable theoretical plates, peak simmetry, peak tailing factor and peak resolution a short run (5 min). The method showed specificity, good linearity (r = 0.9999) and the quantification limit was 0.379 µg mL-1. Furthermore, the method was precise (RSD < 2.0%), accurate (recovery > 97%) and robust.
ISSN:1678-7064

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