Set-up of a method using LC-UV to assay mometasone furoate in pharmaceutical dosage forms

• Main Authors: Aline Ferreira Ourique, Renata Vidor Contri, Silvia Stanisçuaski Guterres, Ruy Carlos Ruver Beck, Adriana Raffin Pohlmann, Ana Melero, Ulrich F. Schaefer
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2012-01-01
• Series: Química Nova
• Subjects: pharmaceuticals; liquid chromatography; mometasone
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000400030&lng=en&tlng=en

A reliable method using LC-UV to assay mometasone furoate (MF) in creams or nasal sprays using the same chromatographic conditions was set up. Methanol:water 80:20 (v/v) (1.0 mL min-1) was used as mobile phase. MF was detected at 248 nm and analyzed in a concentration range from 1.0 to 20.0 µg mL-1. The method provided acceptable theoretical plates, peak simmetry, peak tailing factor and peak resolution a short run (5 min). The method showed specificity, good linearity (r = 0.9999) and the quantification limit was 0.379 µg mL-1. Furthermore, the method was precise (RSD < 2.0%), accurate (recovery > 97%) and robust.