Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain
Abstract Background Typically, carnivores serve as definitive hosts for Sarcocystis spp. parasites; currently, their role as intermediate hosts is being elucidated. The present study aimed to identify and molecularly characterize Sarcocystis cysts detected in striated muscle of red foxes from differ...
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doaj-26c64fa0b47a47b2a20debeda6be6f7f2020-11-25T00:55:10ZengBMCParasites & Vectors1756-33052018-03-011111910.1186/s13071-018-2694-yIdentification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and SpainViktorija Kirillova0Petras Prakas1Rafael Calero-Bernal2Inese Gavarāne3José Luis Fernández-García4Manuel Martínez-González5Eglė Rudaitytė-Lukošienė6Miguel Ángel Habela Martínez-Estéllez7Dalius Butkauskas8Muza Kirjušina9Institute of Life Sciences and Technology, Daugavpils UniversityLaboratory of Molecular Ecology, Nature Research CentreParasitology Section, Faculty of Veterinary Medicine, University of ExtremaduraInstitute of Life Sciences and Technology, Daugavpils UniversityParasitology Section, Faculty of Veterinary Medicine, University of ExtremaduraParasitology Section, Faculty of Veterinary Medicine, University of ExtremaduraLaboratory of Molecular Ecology, Nature Research CentreParasitology Section, Faculty of Veterinary Medicine, University of ExtremaduraLaboratory of Molecular Ecology, Nature Research CentreInstitute of Life Sciences and Technology, Daugavpils UniversityAbstract Background Typically, carnivores serve as definitive hosts for Sarcocystis spp. parasites; currently, their role as intermediate hosts is being elucidated. The present study aimed to identify and molecularly characterize Sarcocystis cysts detected in striated muscle of red foxes from different populations in Latvia, Lithuania and Spain. Methods Muscle samples from 411 red foxes (Vulpes vulpes) and 269 racoon dogs (Nyctereutes procyonoides) from Latvia, 41 red foxes from Lithuania and 22 red foxes from Spain were examined for the presence of Sarcocystis sarcocysts by light microscopy (LM). Sarcocystis spp. were identified by transmission electron microscopy (TEM) and molecular biology techniques. Results Sarcocystis cysts were detected in 11/411 (2.7%) Latvian, 3/41 (7.3%) Lithuanian, and 6/22 (27.3%) Spanish red foxes, however, cysts were not observed in the muscles of racoon dogs. Based on LM, TEM, 18S rDNA, 28S rDNA, ITS1, cox1 and rpoB sequences, Sarcocystis arctica and Sarcocystis lutrae cysts were identified in red fox muscles from Latvia and Lithuania, whereas only S. arctica was detected in Spain. The 18S rDNA, 28S rDNA and ITS1 sequences from the 21 isolates of S. arctica from Latvia, Lithuania and Spain were identical. By contrast, two and four haplotypes were determined based on mtDNA cox1 and apicoplast rpoB sequences, respectively. Polymorphisms were not detected between the two isolates of S. lutrae from Latvia and Lithuania. Based on phylogenetic results, S. arctica and S. lutrae were most closely related to Sarcocystis spp. using predatory mammals as intermediate hosts and to Sarcocystis species with a bird-bird life-cycle. Conclusions Based on current knowledge, the red fox and Arctic fox (Vulpes lagopus) could act as intermediate host for the same two Sarcocystis species. Molecular results suggest the existence of two genetic lineages of S. arctica, and such divergence relies on its geographical distribution but not on their intermediate host species.http://link.springer.com/article/10.1186/s13071-018-2694-ySarcocystis arcticaSarcocystis lutraeWild carnivoresHaplotypesPhylogenyMolecular characterisation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Viktorija Kirillova Petras Prakas Rafael Calero-Bernal Inese Gavarāne José Luis Fernández-García Manuel Martínez-González Eglė Rudaitytė-Lukošienė Miguel Ángel Habela Martínez-Estéllez Dalius Butkauskas Muza Kirjušina |
spellingShingle |
Viktorija Kirillova Petras Prakas Rafael Calero-Bernal Inese Gavarāne José Luis Fernández-García Manuel Martínez-González Eglė Rudaitytė-Lukošienė Miguel Ángel Habela Martínez-Estéllez Dalius Butkauskas Muza Kirjušina Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain Parasites & Vectors Sarcocystis arctica Sarcocystis lutrae Wild carnivores Haplotypes Phylogeny Molecular characterisation |
author_facet |
Viktorija Kirillova Petras Prakas Rafael Calero-Bernal Inese Gavarāne José Luis Fernández-García Manuel Martínez-González Eglė Rudaitytė-Lukošienė Miguel Ángel Habela Martínez-Estéllez Dalius Butkauskas Muza Kirjušina |
author_sort |
Viktorija Kirillova |
title |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_short |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_full |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_fullStr |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_full_unstemmed |
Identification and genetic characterization of Sarcocystis arctica and Sarcocystis lutrae in red foxes (Vulpes vulpes) from Baltic States and Spain |
title_sort |
identification and genetic characterization of sarcocystis arctica and sarcocystis lutrae in red foxes (vulpes vulpes) from baltic states and spain |
publisher |
BMC |
series |
Parasites & Vectors |
issn |
1756-3305 |
publishDate |
2018-03-01 |
description |
Abstract Background Typically, carnivores serve as definitive hosts for Sarcocystis spp. parasites; currently, their role as intermediate hosts is being elucidated. The present study aimed to identify and molecularly characterize Sarcocystis cysts detected in striated muscle of red foxes from different populations in Latvia, Lithuania and Spain. Methods Muscle samples from 411 red foxes (Vulpes vulpes) and 269 racoon dogs (Nyctereutes procyonoides) from Latvia, 41 red foxes from Lithuania and 22 red foxes from Spain were examined for the presence of Sarcocystis sarcocysts by light microscopy (LM). Sarcocystis spp. were identified by transmission electron microscopy (TEM) and molecular biology techniques. Results Sarcocystis cysts were detected in 11/411 (2.7%) Latvian, 3/41 (7.3%) Lithuanian, and 6/22 (27.3%) Spanish red foxes, however, cysts were not observed in the muscles of racoon dogs. Based on LM, TEM, 18S rDNA, 28S rDNA, ITS1, cox1 and rpoB sequences, Sarcocystis arctica and Sarcocystis lutrae cysts were identified in red fox muscles from Latvia and Lithuania, whereas only S. arctica was detected in Spain. The 18S rDNA, 28S rDNA and ITS1 sequences from the 21 isolates of S. arctica from Latvia, Lithuania and Spain were identical. By contrast, two and four haplotypes were determined based on mtDNA cox1 and apicoplast rpoB sequences, respectively. Polymorphisms were not detected between the two isolates of S. lutrae from Latvia and Lithuania. Based on phylogenetic results, S. arctica and S. lutrae were most closely related to Sarcocystis spp. using predatory mammals as intermediate hosts and to Sarcocystis species with a bird-bird life-cycle. Conclusions Based on current knowledge, the red fox and Arctic fox (Vulpes lagopus) could act as intermediate host for the same two Sarcocystis species. Molecular results suggest the existence of two genetic lineages of S. arctica, and such divergence relies on its geographical distribution but not on their intermediate host species. |
topic |
Sarcocystis arctica Sarcocystis lutrae Wild carnivores Haplotypes Phylogeny Molecular characterisation |
url |
http://link.springer.com/article/10.1186/s13071-018-2694-y |
work_keys_str_mv |
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