The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish
Abstract The zinc transporter ZIP9 (SLC39A9) was recently characterized as a membrane androgen receptor in various teleost and mammalian cell models. ZIP9 shows the highest expression in ovaries of teleosts, a tissue in which both androgen signaling and zinc dynamics have significant roles. To exami...
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2020-09-01
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doaj-26c9ba675b2d41c7be443925d056b1832021-09-26T11:21:32ZengNature Publishing GroupScientific Reports2045-23222020-09-0110111410.1038/s41598-020-72515-4The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafishAubrey Converse0Peter Thomas1Marine Science Institute, The University of Texas at AustinMarine Science Institute, The University of Texas at AustinAbstract The zinc transporter ZIP9 (SLC39A9) was recently characterized as a membrane androgen receptor in various teleost and mammalian cell models. ZIP9 shows the highest expression in ovaries of teleosts, a tissue in which both androgen signaling and zinc dynamics have significant roles. To examine the role of ZIP9 in ovarian physiology, we generated a ZIP9-mutant zebrafish strain using a CRISPR/Cas9 system. zip9 -/- females showed significant reductions in fecundity, embryo viability, and growth of their offspring compared to wildtype (WT) fish. Furthermore, a high proportion of zip9 -/- eggs failed to undergo normal chorion elevation during activation. In WT eggs, zinc was detected in cortically-localized vesicles which underwent exocytosis upon activation. zip9 -/- eggs showed abnormal cortical vesicle development and had a significantly depressed activation-induced zinc release compared to WT eggs. Moreover, pharmacologically sustained elevation of zinc in WT eggs prior to activation resulted in abnormal chorion elevation similar to that observed in zip9 -/- eggs. These results indicate that ZIP9 is essential for proper zinc modulation during zebrafish egg activation and presents the first evidence of zinc modulation during egg activation in a non-mammalian species.https://doi.org/10.1038/s41598-020-72515-4 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aubrey Converse Peter Thomas |
spellingShingle |
Aubrey Converse Peter Thomas The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish Scientific Reports |
author_facet |
Aubrey Converse Peter Thomas |
author_sort |
Aubrey Converse |
title |
The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish |
title_short |
The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish |
title_full |
The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish |
title_fullStr |
The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish |
title_full_unstemmed |
The zinc transporter ZIP9 (Slc39a9) regulates zinc dynamics essential to egg activation in zebrafish |
title_sort |
zinc transporter zip9 (slc39a9) regulates zinc dynamics essential to egg activation in zebrafish |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2020-09-01 |
description |
Abstract The zinc transporter ZIP9 (SLC39A9) was recently characterized as a membrane androgen receptor in various teleost and mammalian cell models. ZIP9 shows the highest expression in ovaries of teleosts, a tissue in which both androgen signaling and zinc dynamics have significant roles. To examine the role of ZIP9 in ovarian physiology, we generated a ZIP9-mutant zebrafish strain using a CRISPR/Cas9 system. zip9 -/- females showed significant reductions in fecundity, embryo viability, and growth of their offspring compared to wildtype (WT) fish. Furthermore, a high proportion of zip9 -/- eggs failed to undergo normal chorion elevation during activation. In WT eggs, zinc was detected in cortically-localized vesicles which underwent exocytosis upon activation. zip9 -/- eggs showed abnormal cortical vesicle development and had a significantly depressed activation-induced zinc release compared to WT eggs. Moreover, pharmacologically sustained elevation of zinc in WT eggs prior to activation resulted in abnormal chorion elevation similar to that observed in zip9 -/- eggs. These results indicate that ZIP9 is essential for proper zinc modulation during zebrafish egg activation and presents the first evidence of zinc modulation during egg activation in a non-mammalian species. |
url |
https://doi.org/10.1038/s41598-020-72515-4 |
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