Shared Common Ancestry of Rodent Alphacoronaviruses Sampled Globally
The recent discovery of novel alphacoronaviruses (alpha-CoVs) in European and Asian rodents revealed that rodent coronaviruses (CoVs) sampled worldwide formed a discrete phylogenetic group within this genus. To determine the evolutionary history of rodent CoVs in more detail, particularly the relati...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-01-01
|
Series: | Viruses |
Subjects: | |
Online Access: | https://www.mdpi.com/1999-4915/11/2/125 |
id |
doaj-26cb614b25ce4c119ce742e311886a40 |
---|---|
record_format |
Article |
spelling |
doaj-26cb614b25ce4c119ce742e311886a402020-11-24T21:47:05ZengMDPI AGViruses1999-49152019-01-0111212510.3390/v11020125v11020125Shared Common Ancestry of Rodent Alphacoronaviruses Sampled GloballyTheocharis Tsoleridis0Joseph G. Chappell1Okechukwu Onianwa2Denise A. Marston3Anthony R. Fooks4Elodie Monchatre-Leroy5Gérald Umhang6Marcel A. Müller7Jan F. Drexler8Christian Drosten9Rachael E. Tarlinton10Charles P. McClure11Edward C. Holmes12Jonathan K. Ball13School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKWildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency (APHA), Weybridge-London KT15 3NB, UKWildlife Zoonoses and Vector-borne Diseases Research Group, Animal and Plant Health Agency (APHA), Weybridge-London KT15 3NB, UKAnses, Laboratoire de la rage et de la faune sauvage, 54220 Malzéville, FranceAnses, Laboratoire de la rage et de la faune sauvage, 54220 Malzéville, FranceCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, 10117 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, 10117 Berlin, GermanyCharité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Institute of Virology, 10117 Berlin, GermanySchool of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKMarie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, AustraliaSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKThe recent discovery of novel alphacoronaviruses (alpha-CoVs) in European and Asian rodents revealed that rodent coronaviruses (CoVs) sampled worldwide formed a discrete phylogenetic group within this genus. To determine the evolutionary history of rodent CoVs in more detail, particularly the relative frequencies of virus-host co-divergence and cross-species transmission, we recovered longer fragments of CoV genomes from previously discovered European rodent alpha-CoVs using a combination of PCR and high-throughput sequencing. Accordingly, the full genome sequence was retrieved from the UK rat coronavirus, along with partial genome sequences from the UK field vole and Poland-resident bank vole CoVs, and a short conserved ORF1b fragment from the French rabbit CoV. Genome and phylogenetic analysis showed that despite their diverse geographic origins, all rodent alpha-CoVs formed a single monophyletic group and shared similar features, such as the same gene constellations, a recombinant beta-CoV spike gene, and similar core transcriptional regulatory sequences (TRS). These data suggest that all rodent alpha CoVs sampled so far originate from a single common ancestor, and that there has likely been a long-term association between alpha CoVs and rodents. Despite this likely antiquity, the phylogenetic pattern of the alpha-CoVs was also suggestive of relatively frequent host-jumping among the different rodent species.https://www.mdpi.com/1999-4915/11/2/125coronavirusalphacoronavirusrodentsancestryrecombinationevolution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Theocharis Tsoleridis Joseph G. Chappell Okechukwu Onianwa Denise A. Marston Anthony R. Fooks Elodie Monchatre-Leroy Gérald Umhang Marcel A. Müller Jan F. Drexler Christian Drosten Rachael E. Tarlinton Charles P. McClure Edward C. Holmes Jonathan K. Ball |
spellingShingle |
Theocharis Tsoleridis Joseph G. Chappell Okechukwu Onianwa Denise A. Marston Anthony R. Fooks Elodie Monchatre-Leroy Gérald Umhang Marcel A. Müller Jan F. Drexler Christian Drosten Rachael E. Tarlinton Charles P. McClure Edward C. Holmes Jonathan K. Ball Shared Common Ancestry of Rodent Alphacoronaviruses Sampled Globally Viruses coronavirus alphacoronavirus rodents ancestry recombination evolution |
author_facet |
Theocharis Tsoleridis Joseph G. Chappell Okechukwu Onianwa Denise A. Marston Anthony R. Fooks Elodie Monchatre-Leroy Gérald Umhang Marcel A. Müller Jan F. Drexler Christian Drosten Rachael E. Tarlinton Charles P. McClure Edward C. Holmes Jonathan K. Ball |
author_sort |
Theocharis Tsoleridis |
title |
Shared Common Ancestry of Rodent Alphacoronaviruses Sampled Globally |
title_short |
Shared Common Ancestry of Rodent Alphacoronaviruses Sampled Globally |
title_full |
Shared Common Ancestry of Rodent Alphacoronaviruses Sampled Globally |
title_fullStr |
Shared Common Ancestry of Rodent Alphacoronaviruses Sampled Globally |
title_full_unstemmed |
Shared Common Ancestry of Rodent Alphacoronaviruses Sampled Globally |
title_sort |
shared common ancestry of rodent alphacoronaviruses sampled globally |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2019-01-01 |
description |
The recent discovery of novel alphacoronaviruses (alpha-CoVs) in European and Asian rodents revealed that rodent coronaviruses (CoVs) sampled worldwide formed a discrete phylogenetic group within this genus. To determine the evolutionary history of rodent CoVs in more detail, particularly the relative frequencies of virus-host co-divergence and cross-species transmission, we recovered longer fragments of CoV genomes from previously discovered European rodent alpha-CoVs using a combination of PCR and high-throughput sequencing. Accordingly, the full genome sequence was retrieved from the UK rat coronavirus, along with partial genome sequences from the UK field vole and Poland-resident bank vole CoVs, and a short conserved ORF1b fragment from the French rabbit CoV. Genome and phylogenetic analysis showed that despite their diverse geographic origins, all rodent alpha-CoVs formed a single monophyletic group and shared similar features, such as the same gene constellations, a recombinant beta-CoV spike gene, and similar core transcriptional regulatory sequences (TRS). These data suggest that all rodent alpha CoVs sampled so far originate from a single common ancestor, and that there has likely been a long-term association between alpha CoVs and rodents. Despite this likely antiquity, the phylogenetic pattern of the alpha-CoVs was also suggestive of relatively frequent host-jumping among the different rodent species. |
topic |
coronavirus alphacoronavirus rodents ancestry recombination evolution |
url |
https://www.mdpi.com/1999-4915/11/2/125 |
work_keys_str_mv |
AT theocharistsoleridis sharedcommonancestryofrodentalphacoronavirusessampledglobally AT josephgchappell sharedcommonancestryofrodentalphacoronavirusessampledglobally AT okechukwuonianwa sharedcommonancestryofrodentalphacoronavirusessampledglobally AT deniseamarston sharedcommonancestryofrodentalphacoronavirusessampledglobally AT anthonyrfooks sharedcommonancestryofrodentalphacoronavirusessampledglobally AT elodiemonchatreleroy sharedcommonancestryofrodentalphacoronavirusessampledglobally AT geraldumhang sharedcommonancestryofrodentalphacoronavirusessampledglobally AT marcelamuller sharedcommonancestryofrodentalphacoronavirusessampledglobally AT janfdrexler sharedcommonancestryofrodentalphacoronavirusessampledglobally AT christiandrosten sharedcommonancestryofrodentalphacoronavirusessampledglobally AT rachaeletarlinton sharedcommonancestryofrodentalphacoronavirusessampledglobally AT charlespmcclure sharedcommonancestryofrodentalphacoronavirusessampledglobally AT edwardcholmes sharedcommonancestryofrodentalphacoronavirusessampledglobally AT jonathankball sharedcommonancestryofrodentalphacoronavirusessampledglobally |
_version_ |
1725899474649219072 |