Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing <i>Synechococcus</i> sp. GFB01
Microalgae and cyanobacteria are good sources for prospecting metabolites of biotechnological interest, including glucosidase inhibitors. These inhibitors act on enzymes related to various biochemical processes; they are involved in metabolic diseases, such as diabetes and Gaucher disease, tumors an...
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doaj-26d66f0648a642229ef1deefc2e249972021-08-26T14:05:30ZengMDPI AGMicroorganisms2076-26072021-07-0191593159310.3390/microorganisms9081593Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing <i>Synechococcus</i> sp. GFB01Diana Gomes Gradíssimo0Vivian Cássia Oliveira da Silva1Luciana Pereira Xavier2Sidney Vasconcelos do Nascimento3Rafael Borges da Silva Valadares4Silvia Maria Mathes Faustino5Maria Paula Cruz Schneider6Agenor Valadares Santos7Laboratory of Biotechnology of Enzymes and Biotransformations, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, BrazilLaboratory of Biotechnology of Enzymes and Biotransformations, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, BrazilLaboratory of Biotechnology of Enzymes and Biotransformations, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, BrazilInstituto Tecnológico Vale, Belém 66055-090, BrazilInstituto Tecnológico Vale, Belém 66055-090, BrazilLaboratory of Algae Cultivation and Bioprospecting, Pharmacy Coordination, Marco Zero do Equador Campus, Federal University of Amapá, Juscelino Kubitschek Highway, Km 2, Macapá 68903-419, BrazilGenomics and Systems Biology Center, Federal University of Pará, Belém 66075-110, BrazilLaboratory of Biotechnology of Enzymes and Biotransformations, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, BrazilMicroalgae and cyanobacteria are good sources for prospecting metabolites of biotechnological interest, including glucosidase inhibitors. These inhibitors act on enzymes related to various biochemical processes; they are involved in metabolic diseases, such as diabetes and Gaucher disease, tumors and viral infections, thus, they are interesting hubs for the development of new drugs and therapies. In this work, the screening of 63 environmental samples collected in the Brazilian Amazon found activity against β-glucosidase, of at least 60 min, in 13.85% of the tested extracts, with <i>Synechococcus</i> sp. GFB01 showing inhibitory activity of 90.2% for α-glucosidase and 96.9% against β-glucosidase. It was found that the nutritional limitation due to a reduction in the concentration of sodium nitrate, despite not being sufficient to cause changes in cell growth and photosynthetic apparatus, resulted in reduced production of α and β-glucosidase inhibitors and differential protein expression. The proteomic analysis of cyanobacteria isolated from the Amazon is unprecedented, with this being the first work to evaluate the protein expression of <i>Synechococcus</i> sp. GFB01 subjected to nutritional stress. This evaluation helps to better understand the metabolic responses of this organism, especially related to the production of inhibitors, adding knowledge to the industrial potential of these cyanobacterial compounds.https://www.mdpi.com/2076-2607/9/8/1593glucosidase inhibitorsα-glucosidaseβ-glucosidaseproteomecyanobacteria<i>Synechococcus</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Diana Gomes Gradíssimo Vivian Cássia Oliveira da Silva Luciana Pereira Xavier Sidney Vasconcelos do Nascimento Rafael Borges da Silva Valadares Silvia Maria Mathes Faustino Maria Paula Cruz Schneider Agenor Valadares Santos |
spellingShingle |
Diana Gomes Gradíssimo Vivian Cássia Oliveira da Silva Luciana Pereira Xavier Sidney Vasconcelos do Nascimento Rafael Borges da Silva Valadares Silvia Maria Mathes Faustino Maria Paula Cruz Schneider Agenor Valadares Santos Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing <i>Synechococcus</i> sp. GFB01 Microorganisms glucosidase inhibitors α-glucosidase β-glucosidase proteome cyanobacteria <i>Synechococcus</i> |
author_facet |
Diana Gomes Gradíssimo Vivian Cássia Oliveira da Silva Luciana Pereira Xavier Sidney Vasconcelos do Nascimento Rafael Borges da Silva Valadares Silvia Maria Mathes Faustino Maria Paula Cruz Schneider Agenor Valadares Santos |
author_sort |
Diana Gomes Gradíssimo |
title |
Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing <i>Synechococcus</i> sp. GFB01 |
title_short |
Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing <i>Synechococcus</i> sp. GFB01 |
title_full |
Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing <i>Synechococcus</i> sp. GFB01 |
title_fullStr |
Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing <i>Synechococcus</i> sp. GFB01 |
title_full_unstemmed |
Glucosidase Inhibitors Screening in Microalgae and Cyanobacteria Isolated from the Amazon and Proteomic Analysis of Inhibitor Producing <i>Synechococcus</i> sp. GFB01 |
title_sort |
glucosidase inhibitors screening in microalgae and cyanobacteria isolated from the amazon and proteomic analysis of inhibitor producing <i>synechococcus</i> sp. gfb01 |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2021-07-01 |
description |
Microalgae and cyanobacteria are good sources for prospecting metabolites of biotechnological interest, including glucosidase inhibitors. These inhibitors act on enzymes related to various biochemical processes; they are involved in metabolic diseases, such as diabetes and Gaucher disease, tumors and viral infections, thus, they are interesting hubs for the development of new drugs and therapies. In this work, the screening of 63 environmental samples collected in the Brazilian Amazon found activity against β-glucosidase, of at least 60 min, in 13.85% of the tested extracts, with <i>Synechococcus</i> sp. GFB01 showing inhibitory activity of 90.2% for α-glucosidase and 96.9% against β-glucosidase. It was found that the nutritional limitation due to a reduction in the concentration of sodium nitrate, despite not being sufficient to cause changes in cell growth and photosynthetic apparatus, resulted in reduced production of α and β-glucosidase inhibitors and differential protein expression. The proteomic analysis of cyanobacteria isolated from the Amazon is unprecedented, with this being the first work to evaluate the protein expression of <i>Synechococcus</i> sp. GFB01 subjected to nutritional stress. This evaluation helps to better understand the metabolic responses of this organism, especially related to the production of inhibitors, adding knowledge to the industrial potential of these cyanobacterial compounds. |
topic |
glucosidase inhibitors α-glucosidase β-glucosidase proteome cyanobacteria <i>Synechococcus</i> |
url |
https://www.mdpi.com/2076-2607/9/8/1593 |
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