Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas
The etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study,...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2012-01-01
|
Series: | Journal of Biomedicine and Biotechnology |
Online Access: | http://dx.doi.org/10.1155/2012/823949 |
id |
doaj-26db68a3362145d1aa0593ae073f53ae |
---|---|
record_format |
Article |
spelling |
doaj-26db68a3362145d1aa0593ae073f53ae2020-11-25T01:35:43ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512012-01-01201210.1155/2012/823949823949Detection of Herplex Simplex Virus-1 and -2 in Cardiac MyxomasIoannis S. Pateras0Konstantinos Evangelou1Katerina Tsimaratou2Michalis Liontos3Stratigoula Sakellariou4Theodoros Barlogiannis5Petros Karakitsos6Apostolos Papalois7Athanassios Kotsinas8Vassilis G. Gorgoulis9Molecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National and Kapodistrian University of Athens, 11527, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National and Kapodistrian University of Athens, 11527, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National and Kapodistrian University of Athens, 11527, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National and Kapodistrian University of Athens, 11527, GreeceFirst Department of Pathology, “Laiko” University Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National and Kapodistrian University of Athens, 11527, GreeceDepartment of Cytopathology, “Attikon” University Hospital, 12464 Athens, GreeceExperimental-Research Center, ELPEN Pharma, 19009 Pikermi, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National and Kapodistrian University of Athens, 11527, GreeceMolecular Carcinogenesis Group, Department of Histology and Embryology, School of Medicine, National and Kapodistrian University of Athens, 11527, GreeceThe etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCR-Restriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiac myxomas, whereas no HSV DNA was detected in normal heart tissues (P<0.01). The existence of HSV-1/2 DNA in sporadic cardiac myxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences.http://dx.doi.org/10.1155/2012/823949 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ioannis S. Pateras Konstantinos Evangelou Katerina Tsimaratou Michalis Liontos Stratigoula Sakellariou Theodoros Barlogiannis Petros Karakitsos Apostolos Papalois Athanassios Kotsinas Vassilis G. Gorgoulis |
spellingShingle |
Ioannis S. Pateras Konstantinos Evangelou Katerina Tsimaratou Michalis Liontos Stratigoula Sakellariou Theodoros Barlogiannis Petros Karakitsos Apostolos Papalois Athanassios Kotsinas Vassilis G. Gorgoulis Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas Journal of Biomedicine and Biotechnology |
author_facet |
Ioannis S. Pateras Konstantinos Evangelou Katerina Tsimaratou Michalis Liontos Stratigoula Sakellariou Theodoros Barlogiannis Petros Karakitsos Apostolos Papalois Athanassios Kotsinas Vassilis G. Gorgoulis |
author_sort |
Ioannis S. Pateras |
title |
Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas |
title_short |
Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas |
title_full |
Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas |
title_fullStr |
Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas |
title_full_unstemmed |
Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas |
title_sort |
detection of herplex simplex virus-1 and -2 in cardiac myxomas |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2012-01-01 |
description |
The etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCR-Restriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiac myxomas, whereas no HSV DNA was detected in normal heart tissues (P<0.01). The existence of HSV-1/2 DNA in sporadic cardiac myxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences. |
url |
http://dx.doi.org/10.1155/2012/823949 |
work_keys_str_mv |
AT ioannisspateras detectionofherplexsimplexvirus1and2incardiacmyxomas AT konstantinosevangelou detectionofherplexsimplexvirus1and2incardiacmyxomas AT katerinatsimaratou detectionofherplexsimplexvirus1and2incardiacmyxomas AT michalisliontos detectionofherplexsimplexvirus1and2incardiacmyxomas AT stratigoulasakellariou detectionofherplexsimplexvirus1and2incardiacmyxomas AT theodorosbarlogiannis detectionofherplexsimplexvirus1and2incardiacmyxomas AT petroskarakitsos detectionofherplexsimplexvirus1and2incardiacmyxomas AT apostolospapalois detectionofherplexsimplexvirus1and2incardiacmyxomas AT athanassioskotsinas detectionofherplexsimplexvirus1and2incardiacmyxomas AT vassilisggorgoulis detectionofherplexsimplexvirus1and2incardiacmyxomas |
_version_ |
1725066805353906176 |