VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVE
Background: The effects of demographic factors on a wide range of immunological variables demonstrate the importance of having normative data representative of particular patient population. There was no lymphocyte subpopulation data for Macedonian children and the purpose of this study was to estab...
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doaj-26fc06c9c62f446d906809392085c0c42020-11-24T23:30:42ZengPeytchinski PublishingJournal of IMAB1312-773X2016-12-012241407141010.5272/jimab.2016224.1407VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVELidija Kareva0Kristina Mironska1Katerina Stavrik2Clinic for children’s disease, Department of immunology, Faculty of Medicine - Skopje, Macedonia.Clinic for children’s disease, Department of immunology, Faculty of Medicine - Skopje, Macedonia.Clinic for children’s disease, Department of immunology, Faculty of Medicine - Skopje, Macedonia.Background: The effects of demographic factors on a wide range of immunological variables demonstrate the importance of having normative data representative of particular patient population. There was no lymphocyte subpopulation data for Macedonian children and the purpose of this study was to establish such a data. Subject and methods: The study population consists of 87 healthy children. Subjects were grouped into four age categories as follows: group 1 age range 5d-10d (n=15); group 2 age range 1 mo-1 yr (n=18); group 3 age 1yr-2 yr(n=20) and group 4 age 2yr-5 yr(n=34 Monoclonal antibodies labeled with fluorochromes and immunofluorescent microscopy were used to detect cells bearing specific cell markers. Results: The mean lymphocyte cell count gradually fell with increasing age from 6,65x10^9/l in group 1, to 5,67x10^9/l in group 2, 4,55x10^9/l in group 3, and to 4,14x10^9/l in group 4. Absolute values of CD3, CD4 and CD20 positive cells decreased gradually with age. Significant differences in mean absolute values were observed for absolute lymphocyte counts between groups 1/2 and 1/4 (P<0,01) and groups 2/4(P<0,05); for CD3 positive lymphocytes between groups 1/3(P<0,05) and 1/4(P<0,01); for CD4 positive lymphocytes between groups 1/3 (P<0,05) and 1/4(P<0,01) and for CD20 positive lymphocytes between groups 1/3 and 3/4(P<0,05) and groups 1/4 (P<0,01). Significant difference for CD4/CD8 ratio and for percentage values of different lymphocyte subpopulations between the different age groups was not found. Conclusion: This data may serve as a reference range for studies of Macedonian pediatric subjects.http://www.journal-imab-bg.org/issues-2016/issue4/JofIMAB-2016-22-4p1407-1410.pdflymphocyte subpopulationchildren |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lidija Kareva Kristina Mironska Katerina Stavrik |
spellingShingle |
Lidija Kareva Kristina Mironska Katerina Stavrik VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVE Journal of IMAB lymphocyte subpopulation children |
author_facet |
Lidija Kareva Kristina Mironska Katerina Stavrik |
author_sort |
Lidija Kareva |
title |
VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVE |
title_short |
VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVE |
title_full |
VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVE |
title_fullStr |
VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVE |
title_full_unstemmed |
VALUES OF LYMPHOCYTE SUBPOPULATIONS IN HEALTHY MACEDONIAN CHILDREN UNDER THE AGE OF FIVE |
title_sort |
values of lymphocyte subpopulations in healthy macedonian children under the age of five |
publisher |
Peytchinski Publishing |
series |
Journal of IMAB |
issn |
1312-773X |
publishDate |
2016-12-01 |
description |
Background: The effects of demographic factors on a wide range of immunological variables demonstrate the importance of having normative data representative of particular patient population. There was no lymphocyte subpopulation data for Macedonian children and the purpose of this study was to establish such a data.
Subject and methods: The study population consists of 87 healthy children. Subjects were grouped into four age categories as follows: group 1 age range 5d-10d (n=15); group 2 age range 1 mo-1 yr (n=18); group 3 age 1yr-2 yr(n=20) and group 4 age 2yr-5 yr(n=34
Monoclonal antibodies labeled with fluorochromes and immunofluorescent microscopy were used to detect cells bearing specific cell markers.
Results: The mean lymphocyte cell count gradually fell with increasing age from 6,65x10^9/l in group 1, to 5,67x10^9/l in group 2, 4,55x10^9/l in group 3, and to 4,14x10^9/l in group 4. Absolute values of CD3, CD4 and CD20 positive cells decreased gradually with age. Significant differences in mean absolute values were observed for absolute lymphocyte counts between groups 1/2 and 1/4 (P<0,01) and groups 2/4(P<0,05); for CD3 positive lymphocytes between groups 1/3(P<0,05) and 1/4(P<0,01); for CD4 positive lymphocytes between groups 1/3 (P<0,05) and 1/4(P<0,01) and for CD20 positive lymphocytes between groups 1/3 and 3/4(P<0,05) and groups 1/4 (P<0,01). Significant difference for CD4/CD8 ratio and for percentage values of different lymphocyte subpopulations between the different age groups was not found.
Conclusion: This data may serve as a reference range for studies of Macedonian pediatric subjects. |
topic |
lymphocyte subpopulation children |
url |
http://www.journal-imab-bg.org/issues-2016/issue4/JofIMAB-2016-22-4p1407-1410.pdf |
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AT lidijakareva valuesoflymphocytesubpopulationsinhealthymacedonianchildrenundertheageoffive AT kristinamironska valuesoflymphocytesubpopulationsinhealthymacedonianchildrenundertheageoffive AT katerinastavrik valuesoflymphocytesubpopulationsinhealthymacedonianchildrenundertheageoffive |
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