Spectral parameters and biological activity of macromolecular compounds of humic etiology
Materials and methods. 18 native humic acids (HAs) were received from nine representative types of peat of the Tomsk region. Two extraction methods were used: sodium hydroxide and sodium pyrophosphate. Molecular structure parameters were investigated by IR-spectroscopy. The assessement of qualitativ...
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doaj-26fe55d760784dd8b390845c23a1e3132021-07-29T08:38:03ZengSiberian State Medical University (Tomsk)Bûlleten' Sibirskoj Mediciny1682-03631819-36842017-04-01161364910.20538/1682-0363-2017-1-36-49620Spectral parameters and biological activity of macromolecular compounds of humic etiologyM. V. Zykova0E. S. Trofimova1S. V. Krivoshchekov2A. A. Ligacheva3M. G. Danilets4L. A. Logvinova5G. A. Zholobova6O. F. Prishchepova7M. S. Yusubov8M. V. Belousov9Siberian State Medical University (SSMU)Goldberg Research Institute of Pharmacology and Regenerative Medicine (GRIPRM), Tomsk National Research Medical Center (TNRMC), Russian Academy of Sciences (RAS)Siberian State Medical University (SSMU); National Research Tomsk Polytechnic University (NR TPU)Goldberg Research Institute of Pharmacology and Regenerative Medicine (GRIPRM), Tomsk National Research Medical Center (TNRMC), Russian Academy of Sciences (RAS)Goldberg Research Institute of Pharmacology and Regenerative Medicine (GRIPRM), Tomsk National Research Medical Center (TNRMC), Russian Academy of Sciences (RAS)Siberian State Medical University (SSMU)Siberian State Medical University (SSMU)Siberian State Medical University (SSMU)Siberian State Medical University (SSMU); National Research Tomsk Polytechnic University (NR TPU)Siberian State Medical University (SSMU)Materials and methods. 18 native humic acids (HAs) were received from nine representative types of peat of the Tomsk region. Two extraction methods were used: sodium hydroxide and sodium pyrophosphate. Molecular structure parameters were investigated by IR-spectroscopy. The assessement of qualitative and quantitative features of the IR-spectra of 18 different humic acids was made. When HAs with mouse macrophages were cultured their ability to influence the NO-stimulation was determined. Thus, the biological activity of HAs and its dependence on the parameters of the molecular structure were studied.Results. The results of infrared spectroscopy showed that the HAs of upland types of peat contain more carbonyl, carboxyl, and ester groups, and HAs of lowland types of peat contain more aromatic carbon, phenolic and alcoholic hydroxyl, ether and carbohydrate fragments. The results of biological activity showed that HAs from upland types of peat induce the formation of nitrogen oxide, wherein the cell activation decreases with HAs obtained by alkali. All types of HAs from lowland types of peat contain an admixture of endotoxin. Some HAs obtained by sodium pyrophosphate have higher immunotropic activity; the HAs can cause antigen-specific stimulation of cells. The activity of HAs does not depend on endotoxin admixture. The results of molecular spectroscopy showed that the most biologically active HAs have higher aromaticity and higher concentration of oxygen-containing functional groups. This result can be used as a marker factor in the standardization of HAs.https://bulletin.tomsk.ru/jour/article/view/772humic acidspeatmolecular spectroscopymacrophagesnitric oxide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. V. Zykova E. S. Trofimova S. V. Krivoshchekov A. A. Ligacheva M. G. Danilets L. A. Logvinova G. A. Zholobova O. F. Prishchepova M. S. Yusubov M. V. Belousov |
spellingShingle |
M. V. Zykova E. S. Trofimova S. V. Krivoshchekov A. A. Ligacheva M. G. Danilets L. A. Logvinova G. A. Zholobova O. F. Prishchepova M. S. Yusubov M. V. Belousov Spectral parameters and biological activity of macromolecular compounds of humic etiology Bûlleten' Sibirskoj Mediciny humic acids peat molecular spectroscopy macrophages nitric oxide |
author_facet |
M. V. Zykova E. S. Trofimova S. V. Krivoshchekov A. A. Ligacheva M. G. Danilets L. A. Logvinova G. A. Zholobova O. F. Prishchepova M. S. Yusubov M. V. Belousov |
author_sort |
M. V. Zykova |
title |
Spectral parameters and biological activity of macromolecular compounds of humic etiology |
title_short |
Spectral parameters and biological activity of macromolecular compounds of humic etiology |
title_full |
Spectral parameters and biological activity of macromolecular compounds of humic etiology |
title_fullStr |
Spectral parameters and biological activity of macromolecular compounds of humic etiology |
title_full_unstemmed |
Spectral parameters and biological activity of macromolecular compounds of humic etiology |
title_sort |
spectral parameters and biological activity of macromolecular compounds of humic etiology |
publisher |
Siberian State Medical University (Tomsk) |
series |
Bûlleten' Sibirskoj Mediciny |
issn |
1682-0363 1819-3684 |
publishDate |
2017-04-01 |
description |
Materials and methods. 18 native humic acids (HAs) were received from nine representative types of peat of the Tomsk region. Two extraction methods were used: sodium hydroxide and sodium pyrophosphate. Molecular structure parameters were investigated by IR-spectroscopy. The assessement of qualitative and quantitative features of the IR-spectra of 18 different humic acids was made. When HAs with mouse macrophages were cultured their ability to influence the NO-stimulation was determined. Thus, the biological activity of HAs and its dependence on the parameters of the molecular structure were studied.Results. The results of infrared spectroscopy showed that the HAs of upland types of peat contain more carbonyl, carboxyl, and ester groups, and HAs of lowland types of peat contain more aromatic carbon, phenolic and alcoholic hydroxyl, ether and carbohydrate fragments. The results of biological activity showed that HAs from upland types of peat induce the formation of nitrogen oxide, wherein the cell activation decreases with HAs obtained by alkali. All types of HAs from lowland types of peat contain an admixture of endotoxin. Some HAs obtained by sodium pyrophosphate have higher immunotropic activity; the HAs can cause antigen-specific stimulation of cells. The activity of HAs does not depend on endotoxin admixture. The results of molecular spectroscopy showed that the most biologically active HAs have higher aromaticity and higher concentration of oxygen-containing functional groups. This result can be used as a marker factor in the standardization of HAs. |
topic |
humic acids peat molecular spectroscopy macrophages nitric oxide |
url |
https://bulletin.tomsk.ru/jour/article/view/772 |
work_keys_str_mv |
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