Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715
The objective of this work is to clone flanking DNA derived from Tn-5 mutagenesis of wild type strain Xanthomonas axonopodis pv. glycines as first step to clone and to identify the gene involved in pathogenicity mechanism. We have localized the flanking DNA fragment from a nonpathogenic mutant of Xa...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Bogor Agricultural University
2005-06-01
|
Series: | Hayati Journal of Biosciences |
Subjects: | |
Online Access: | http://journal.ipb.ac.id/index.php/hayati/article/viewFile/173/40 |
id |
doaj-270589d576b342f6b0805637e6cf886a |
---|---|
record_format |
Article |
spelling |
doaj-270589d576b342f6b0805637e6cf886a2020-11-24T23:46:19ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942005-06-011225760Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715ALINA AKHDIYA RUSMANAANTONIUS SUWANTOBUDI TJAHJONOThe objective of this work is to clone flanking DNA derived from Tn-5 mutagenesis of wild type strain Xanthomonas axonopodis pv. glycines as first step to clone and to identify the gene involved in pathogenicity mechanism. We have localized the flanking DNA fragment from a nonpathogenic mutant of Xag M715. Southern hybridization analysis using 2.8 kb EcoRI from pYR103 as a probe showed that the fragment is located within 2.0 kb PstI fragment. A 0.7 kb flanking DNA was amplified using inverse PCR technique, and inserted into pGEM-T Easy vector generating a 3.7 kb recombinant plasmid (pAA01). Southern hybridization analysis of the wild type (YR32) with pAA01 as a probe indicated a hybridization signal located at approximately 3.0 kb PstI fragment. DNA sequence analysis revealed that the DNA fragment has a 64% identity to a vir gene of Bacillus anthracis.http://journal.ipb.ac.id/index.php/hayati/article/viewFile/173/40CloningDNA Flanking TransposonXanthomonas axonopodispv. glycines |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
ALINA AKHDIYA RUSMANA ANTONIUS SUWANTO BUDI TJAHJONO |
spellingShingle |
ALINA AKHDIYA RUSMANA ANTONIUS SUWANTO BUDI TJAHJONO Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715 Hayati Journal of Biosciences Cloning DNA Flanking Transposon Xanthomonas axonopodis pv. glycines |
author_facet |
ALINA AKHDIYA RUSMANA ANTONIUS SUWANTO BUDI TJAHJONO |
author_sort |
ALINA AKHDIYA RUSMANA |
title |
Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715 |
title_short |
Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715 |
title_full |
Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715 |
title_fullStr |
Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715 |
title_full_unstemmed |
Cloning of Genomic DNA Flanking Transposon in the Nonpathogenic Mutant of Xanthomonas axonopodis pv. glycines M715 |
title_sort |
cloning of genomic dna flanking transposon in the nonpathogenic mutant of xanthomonas axonopodis pv. glycines m715 |
publisher |
Bogor Agricultural University |
series |
Hayati Journal of Biosciences |
issn |
1978-3019 2086-4094 |
publishDate |
2005-06-01 |
description |
The objective of this work is to clone flanking DNA derived from Tn-5 mutagenesis of wild type strain Xanthomonas axonopodis pv. glycines as first step to clone and to identify the gene involved in pathogenicity mechanism. We have localized the flanking DNA fragment from a nonpathogenic mutant of Xag M715. Southern hybridization analysis using 2.8 kb EcoRI from pYR103 as a probe showed that the fragment is located within 2.0 kb PstI fragment. A 0.7 kb flanking DNA was amplified using inverse PCR technique, and inserted into pGEM-T Easy vector generating a 3.7 kb recombinant plasmid (pAA01). Southern hybridization analysis of the wild type (YR32) with pAA01 as a probe indicated a hybridization signal located at approximately 3.0 kb PstI fragment. DNA sequence analysis revealed that the DNA fragment has a 64% identity to a vir gene of Bacillus anthracis. |
topic |
Cloning DNA Flanking Transposon Xanthomonas axonopodis pv. glycines |
url |
http://journal.ipb.ac.id/index.php/hayati/article/viewFile/173/40 |
work_keys_str_mv |
AT alinaakhdiyarusmana cloningofgenomicdnaflankingtransposoninthenonpathogenicmutantofxanthomonasaxonopodispvglycinesm715 AT antoniussuwanto cloningofgenomicdnaflankingtransposoninthenonpathogenicmutantofxanthomonasaxonopodispvglycinesm715 AT buditjahjono cloningofgenomicdnaflankingtransposoninthenonpathogenicmutantofxanthomonasaxonopodispvglycinesm715 |
_version_ |
1725493699402530816 |