Efficacy of Ozonated Water, Chlorine, Chlorine Dioxide, Quaternary Ammonium Compounds and Peroxyacetic Acid Against Listeria monocytogenes Biofilm on Polystyrene Surfaces
Listeria monocytogenes contaminated processing equipment and the general packing environment have been implicated in deadly foodborne listeriosis outbreaks, highlighting the significance of proper sanitization and disinfection of food contact surfaces. This study aims to comprehensively evaluate ant...
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doaj-270ac2c2e1de405983275001d380a3e82020-11-25T01:32:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02296411379Efficacy of Ozonated Water, Chlorine, Chlorine Dioxide, Quaternary Ammonium Compounds and Peroxyacetic Acid Against Listeria monocytogenes Biofilm on Polystyrene SurfacesAhmed Mahmoud Korany0Ahmed Mahmoud Korany1Zi Hua2Tonia Green3Ines Hanrahan4Saadia Helmy El-Shinawy5Adel El-kholy6Gamal Hassan7Mei-Jun Zhu8School of Food Science, Washington State University, Pullman, WA, United StatesFood Hygiene and Control Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, EgyptSchool of Food Science, Washington State University, Pullman, WA, United StatesSchool of Food Science, Washington State University, Pullman, WA, United StatesWashington Tree Fruit Research Commission, Wenatchee, WA, United StatesFood Hygiene and Control Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, EgyptFood Hygiene and Control Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, EgyptFood Hygiene and Control Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, EgyptSchool of Food Science, Washington State University, Pullman, WA, United StatesListeria monocytogenes contaminated processing equipment and the general packing environment have been implicated in deadly foodborne listeriosis outbreaks, highlighting the significance of proper sanitization and disinfection of food contact surfaces. This study aims to comprehensively evaluate antimicrobial efficacy of commercially available, economical sanitizers at practical concentrations against L. monocytogenes biofilm formed on polystyrene surfaces under different conditions. Ozonated water 1-min treatment at 1.0, 2.0, and 4.0 ppm resulted in ∼0.9, 3.4, and 4.1 log reduction of L. monocytogenes single strain biofilm grown on polystyrene surfaces, respectively. However, its efficacy was dramatically diminished in multi-strain L. monocytogenes biofilm and was further compromised by aged biofilm and in the presence of organic matter. Quaternary ammonium compounds (QAC) at 100/400 ppm, chlorine at 100/200 ppm, chlorine dioxide at 2.5/5.0 ppm and peroxyacetic acid (PAA) at 80/160 ppm resulted in 2.4/3.6, 2.0/3.1, 2.4/3.8, and 3.6/4.8 log reduction of L. monocytogenes single strain biofilm, respectively. Antimicrobial efficacies of all tested sanitizers against 7-day-old biofilm were much lower when compared to 2-day-old biofilm, with PAA being the least influenced by the age of the biofilm. Organic matter conditioning with diluted milk or apple juice dramatically impacted the antimicrobial efficacy of all sanitizers. PAA treatment of 1 min at 160–200 ppm resulted in a 3.2–3.5 log reduction against 7-day-old biofilm in the presence of organic matter, thus showing its effectiveness in eradicating L. monocytogenes biofilm on polystyrene surface. Collectively, data highlight the importance of timely and thoroughly cleaning food contact surfaces before disinfection and provides practical information and guidance for the food industry in selecting the most effective sanitizer in their sanitizing regimes to eliminate L. monocytogenes biofilm.https://www.frontiersin.org/article/10.3389/fmicb.2018.02296/fullbiofilmL. monocytogenessanitizersperoxyacetic acidpolystyreneorganic matter |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed Mahmoud Korany Ahmed Mahmoud Korany Zi Hua Tonia Green Ines Hanrahan Saadia Helmy El-Shinawy Adel El-kholy Gamal Hassan Mei-Jun Zhu |
spellingShingle |
Ahmed Mahmoud Korany Ahmed Mahmoud Korany Zi Hua Tonia Green Ines Hanrahan Saadia Helmy El-Shinawy Adel El-kholy Gamal Hassan Mei-Jun Zhu Efficacy of Ozonated Water, Chlorine, Chlorine Dioxide, Quaternary Ammonium Compounds and Peroxyacetic Acid Against Listeria monocytogenes Biofilm on Polystyrene Surfaces Frontiers in Microbiology biofilm L. monocytogenes sanitizers peroxyacetic acid polystyrene organic matter |
author_facet |
Ahmed Mahmoud Korany Ahmed Mahmoud Korany Zi Hua Tonia Green Ines Hanrahan Saadia Helmy El-Shinawy Adel El-kholy Gamal Hassan Mei-Jun Zhu |
author_sort |
Ahmed Mahmoud Korany |
title |
Efficacy of Ozonated Water, Chlorine, Chlorine Dioxide, Quaternary Ammonium Compounds and Peroxyacetic Acid Against Listeria monocytogenes Biofilm on Polystyrene Surfaces |
title_short |
Efficacy of Ozonated Water, Chlorine, Chlorine Dioxide, Quaternary Ammonium Compounds and Peroxyacetic Acid Against Listeria monocytogenes Biofilm on Polystyrene Surfaces |
title_full |
Efficacy of Ozonated Water, Chlorine, Chlorine Dioxide, Quaternary Ammonium Compounds and Peroxyacetic Acid Against Listeria monocytogenes Biofilm on Polystyrene Surfaces |
title_fullStr |
Efficacy of Ozonated Water, Chlorine, Chlorine Dioxide, Quaternary Ammonium Compounds and Peroxyacetic Acid Against Listeria monocytogenes Biofilm on Polystyrene Surfaces |
title_full_unstemmed |
Efficacy of Ozonated Water, Chlorine, Chlorine Dioxide, Quaternary Ammonium Compounds and Peroxyacetic Acid Against Listeria monocytogenes Biofilm on Polystyrene Surfaces |
title_sort |
efficacy of ozonated water, chlorine, chlorine dioxide, quaternary ammonium compounds and peroxyacetic acid against listeria monocytogenes biofilm on polystyrene surfaces |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2018-10-01 |
description |
Listeria monocytogenes contaminated processing equipment and the general packing environment have been implicated in deadly foodborne listeriosis outbreaks, highlighting the significance of proper sanitization and disinfection of food contact surfaces. This study aims to comprehensively evaluate antimicrobial efficacy of commercially available, economical sanitizers at practical concentrations against L. monocytogenes biofilm formed on polystyrene surfaces under different conditions. Ozonated water 1-min treatment at 1.0, 2.0, and 4.0 ppm resulted in ∼0.9, 3.4, and 4.1 log reduction of L. monocytogenes single strain biofilm grown on polystyrene surfaces, respectively. However, its efficacy was dramatically diminished in multi-strain L. monocytogenes biofilm and was further compromised by aged biofilm and in the presence of organic matter. Quaternary ammonium compounds (QAC) at 100/400 ppm, chlorine at 100/200 ppm, chlorine dioxide at 2.5/5.0 ppm and peroxyacetic acid (PAA) at 80/160 ppm resulted in 2.4/3.6, 2.0/3.1, 2.4/3.8, and 3.6/4.8 log reduction of L. monocytogenes single strain biofilm, respectively. Antimicrobial efficacies of all tested sanitizers against 7-day-old biofilm were much lower when compared to 2-day-old biofilm, with PAA being the least influenced by the age of the biofilm. Organic matter conditioning with diluted milk or apple juice dramatically impacted the antimicrobial efficacy of all sanitizers. PAA treatment of 1 min at 160–200 ppm resulted in a 3.2–3.5 log reduction against 7-day-old biofilm in the presence of organic matter, thus showing its effectiveness in eradicating L. monocytogenes biofilm on polystyrene surface. Collectively, data highlight the importance of timely and thoroughly cleaning food contact surfaces before disinfection and provides practical information and guidance for the food industry in selecting the most effective sanitizer in their sanitizing regimes to eliminate L. monocytogenes biofilm. |
topic |
biofilm L. monocytogenes sanitizers peroxyacetic acid polystyrene organic matter |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2018.02296/full |
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