Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus
Pre-immunization with mild strains of Papaya ringspot virus - type W (PRWV-W) has allowed the mosaic disease to be controlled in different cucurbit species, with increases in marketable fruit yield. The objective of this study was to compare virus concentration, biomass and symptomatology of 'C...
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doaj-270e8d0f144d4934927825bfa915408b2020-11-24T22:46:52ZengUniversidade de São PauloScientia Agricola0103-90161678-992X2003-01-01604691698Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virusPacheco Davi AndradeRezende Jorge Alberto MarquesPiedade Sônia Maria de StefanoPre-immunization with mild strains of Papaya ringspot virus - type W (PRWV-W) has allowed the mosaic disease to be controlled in different cucurbit species, with increases in marketable fruit yield. The objective of this study was to compare virus concentration, biomass and symptomatology of 'Caserta' zucchini squash, 'Menina Brasileira' long-neck squash and 'Crimson Sweet' watermelon plants infected by three mild strains and one severe strain of PRSV-W. Plants were inoculated at the cotyledonary stage, under greenhouse conditions, sampled at 7, 14, 21, 28 and 35 days after inoculation (DAI), and analyzed by PTA-ELISA. The severity of the symptoms was scored according to a scale from 1 to 5, and the fresh and dry biomass of the aerial part of the plants were evaluated at 40 DAI. Concentrations of the mild strains, based on absorbance values of the PTA-ELISA, were lower than the concentration of the severe strain for all species. The mild strains did not cause mosaic in infected plants of all species. Plants of zucchini squash and watermelon infected by the severe strain exhibited severe mosaic symptoms, but the same was not noticed for infected long-neck squash plants. Biomass values from zucchini squash and watermelon plants infected by the mild strains were 1.7 % to 12.4 % lower as compared to healthy plants. Biomass values of zucchini squash and watermelon plants infected by the severe strain presented greater reduction, varying from 29 % to 74 %. However, biomass values of long-neck squash plants infected by the mild and severe strains were similar for all treatments.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400013PTA-ELISACucurbita pepoCucurbita moschataCitrullus lanatus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pacheco Davi Andrade Rezende Jorge Alberto Marques Piedade Sônia Maria de Stefano |
spellingShingle |
Pacheco Davi Andrade Rezende Jorge Alberto Marques Piedade Sônia Maria de Stefano Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus Scientia Agricola PTA-ELISA Cucurbita pepo Cucurbita moschata Citrullus lanatus |
author_facet |
Pacheco Davi Andrade Rezende Jorge Alberto Marques Piedade Sônia Maria de Stefano |
author_sort |
Pacheco Davi Andrade |
title |
Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus |
title_short |
Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus |
title_full |
Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus |
title_fullStr |
Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus |
title_full_unstemmed |
Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus |
title_sort |
biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of papaya ringspot virus |
publisher |
Universidade de São Paulo |
series |
Scientia Agricola |
issn |
0103-9016 1678-992X |
publishDate |
2003-01-01 |
description |
Pre-immunization with mild strains of Papaya ringspot virus - type W (PRWV-W) has allowed the mosaic disease to be controlled in different cucurbit species, with increases in marketable fruit yield. The objective of this study was to compare virus concentration, biomass and symptomatology of 'Caserta' zucchini squash, 'Menina Brasileira' long-neck squash and 'Crimson Sweet' watermelon plants infected by three mild strains and one severe strain of PRSV-W. Plants were inoculated at the cotyledonary stage, under greenhouse conditions, sampled at 7, 14, 21, 28 and 35 days after inoculation (DAI), and analyzed by PTA-ELISA. The severity of the symptoms was scored according to a scale from 1 to 5, and the fresh and dry biomass of the aerial part of the plants were evaluated at 40 DAI. Concentrations of the mild strains, based on absorbance values of the PTA-ELISA, were lower than the concentration of the severe strain for all species. The mild strains did not cause mosaic in infected plants of all species. Plants of zucchini squash and watermelon infected by the severe strain exhibited severe mosaic symptoms, but the same was not noticed for infected long-neck squash plants. Biomass values from zucchini squash and watermelon plants infected by the mild strains were 1.7 % to 12.4 % lower as compared to healthy plants. Biomass values of zucchini squash and watermelon plants infected by the severe strain presented greater reduction, varying from 29 % to 74 %. However, biomass values of long-neck squash plants infected by the mild and severe strains were similar for all treatments. |
topic |
PTA-ELISA Cucurbita pepo Cucurbita moschata Citrullus lanatus |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400013 |
work_keys_str_mv |
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