Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract

Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract

This data article reports changes in the phosphoproteome and total proteome of cigarette smoke extract (CSE) exposed WT and MAP Kinase Kinase 3 knock out (MKK3−/−) bone marrow derived macrophages (BMDM). The dataset generated is helpful for understanding the mechanism of CSE induced inflammation and...

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Main Authors: Roshni Srivastava, Praveen Mannam, Navin Rauniyar, TuKiet T. Lam, Ruiyan Luo, Patty J. Lee, Anup Srivastava
Format: Article
Language:English
Published: Elsevier 2017-08-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340917302408
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spelling doaj-271751eda2c94bbdb97605716be6c9612020-11-25T02:29:24ZengElsevierData in Brief2352-34092017-08-0113320325Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extractRoshni Srivastava0Praveen Mannam1Navin Rauniyar2TuKiet T. Lam3Ruiyan Luo4Patty J. Lee5Anup Srivastava6Department of Internal Medicine, Yale University School of Medicine, USADepartment of Internal Medicine, Yale University School of Medicine, USAMS & Proteomics Resource at Yale University, WM Keck Foundation Biotechnology Resource Laboratory, Department of Molecular Biophysics and Biochemistry, New Haven, CT, USAMS & Proteomics Resource at Yale University, WM Keck Foundation Biotechnology Resource Laboratory, Department of Molecular Biophysics and Biochemistry, New Haven, CT, USADepartment of Epidemiology & Biostatistics, School of Public Health, Georgia State University, Atlanta, GA, USADepartment of Internal Medicine, Yale University School of Medicine, USADivision of Translational and Regenerative Medicine, Internal Medicine, University of Arizona, Tucson, AZ, USA; Corresponding author.This data article reports changes in the phosphoproteome and total proteome of cigarette smoke extract (CSE) exposed WT and MAP Kinase Kinase 3 knock out (MKK3−/−) bone marrow derived macrophages (BMDM). The dataset generated is helpful for understanding the mechanism of CSE induced inflammation and the role of MAP kinase signaling pathway. The cellular proteins were labeled with isobaric tags for relative and absolute quantitation (iTRAQ®) reagents and analyzed by LC-MS/MS. The standard workflow module for iTRAQ® quantification within the Proteome Discoverer was utilized for the data analysis. Ingenuity Pathway Analysis (IPA) software and Reactome was used to identify enriched canonical pathways and molecular networks (Mannam et al., 2016) [1]. All the associated mass spectrometry data has been deposited in the Yale Protein Expression Database (YPED) with the web-link to the data: http://yped.med.yale.edu/repository/ViewSeriesMenu.do;jsessionid=6A5CB07543D8B529FAE8C3FCFE29471D?series_id=5044&series_name=MMK3+Deletion+in+MEFs Keywords: MKK3, Cigarette smoke, Inflammation, Proteomics, iTRAQ®http://www.sciencedirect.com/science/article/pii/S2352340917302408
collection DOAJ
language English
format Article
sources DOAJ
author Roshni Srivastava
Praveen Mannam
Navin Rauniyar
TuKiet T. Lam
Ruiyan Luo
Patty J. Lee
Anup Srivastava
spellingShingle Roshni Srivastava
Praveen Mannam
Navin Rauniyar
TuKiet T. Lam
Ruiyan Luo
Patty J. Lee
Anup Srivastava
Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract
Data in Brief
author_facet Roshni Srivastava
Praveen Mannam
Navin Rauniyar
TuKiet T. Lam
Ruiyan Luo
Patty J. Lee
Anup Srivastava
author_sort Roshni Srivastava
title Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract
title_short Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract
title_full Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract
title_fullStr Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract
title_full_unstemmed Proteomics data on MAP Kinase Kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract
title_sort proteomics data on map kinase kinase 3 knock out bone marrow derived macrophages exposed to cigarette smoke extract
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2017-08-01
description This data article reports changes in the phosphoproteome and total proteome of cigarette smoke extract (CSE) exposed WT and MAP Kinase Kinase 3 knock out (MKK3−/−) bone marrow derived macrophages (BMDM). The dataset generated is helpful for understanding the mechanism of CSE induced inflammation and the role of MAP kinase signaling pathway. The cellular proteins were labeled with isobaric tags for relative and absolute quantitation (iTRAQ®) reagents and analyzed by LC-MS/MS. The standard workflow module for iTRAQ® quantification within the Proteome Discoverer was utilized for the data analysis. Ingenuity Pathway Analysis (IPA) software and Reactome was used to identify enriched canonical pathways and molecular networks (Mannam et al., 2016) [1]. All the associated mass spectrometry data has been deposited in the Yale Protein Expression Database (YPED) with the web-link to the data: http://yped.med.yale.edu/repository/ViewSeriesMenu.do;jsessionid=6A5CB07543D8B529FAE8C3FCFE29471D?series_id=5044&series_name=MMK3+Deletion+in+MEFs Keywords: MKK3, Cigarette smoke, Inflammation, Proteomics, iTRAQ®
url http://www.sciencedirect.com/science/article/pii/S2352340917302408
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