Genetic Relationships Among Representatives of Dasypyrum, Secale and Triticum Species Revealed with RAPD and ISSR Markers

• Main Authors: Agnieszka GRADZIELEWSKA, Mirosław TYRKA, Justyna LEŚNIOWSKA-NOWAK, Justyna NAZARUK
• Format: Article
• Language: English
• Published: AcademicPres 2014-12-01
• Series: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
• Online Access: http://www.notulaebotanicae.ro/index.php/nbha/article/view/9662
• ISSN: 0255-965X; 1842-4309

<p><span lang="EN-US" style="font-size: 12.0pt; font-family: 'Garamond Premr Pro',serif">In this study the genetic similarity among <i>Dasypyrum</i>, <i>Secale</i> and<i>Triticum</i> species with RAPDs and ISSRs was analyzed. To show a level of similarity<i> </i>between<i> </i>the species, 12 populations of <i>Dasypyrum </i>(11 <i>D. villosum</i> and 1 <i>D. breviaristatum</i>), together with 12 accessions belonging to 3 <i>Secale</i> species and 12 accessions from 4 <i>Triticum</i> species were used. Genetic distances (GD) and bootstrap values were calculated and PCA analysis was conducted to present the relationships among the species. To estimate the genetic structure among and inside genera, as well as population differentiation, gene diversity (He), total genetic variation (Ht) and Wright’s fixation index (Fst) were computed. The highest values were found in <i>Triticum, </i>within which Ht was equal to 0.332±0.023 and Fst was 0.42. It confirmed that the material studied was highly differentiated. Both systems found <i>Dasypyrum</i> more related with <i>Triticum</i>, as compared to <i>Secale</i>. With RAPDs, genetic distance (GD) between <i>Triticum</i> and <i>Dasypyrum</i> was 0.435, respectively 0.460 for <i>Secale </i>vs <i>Dasypyrum</i>. In the case of ISSRs these values were 0.374 and 0.407, respectively. Despite the fact that the difference between the two GD indices was insignificant, one should not exclude the possibility of successful hybridization of <i>Dasypyrum</i> and <i>Secale</i>, especially when using bridge species.</span></p>