Influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from Leuconostoc mesenteroides Lm 28
A study of modulation of the reactions catalyzed by dextransucrase from Leuconostoc mesenteroides Lm 28 strain in the presence of triterpenoid ursolic acid was carried out. This compound showed concentration dependent inhibition of the studied dextransucrase and Ki = 1.9 mM, which is about 5 times h...
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2015-08-01
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doaj-2755342dcf184067a8df484df468c5822020-11-25T02:15:04ZengPlovdiv University Press Journal of BioScience and Biotechnology1314-62381314-62462015-08-0142175183Influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from Leuconostoc mesenteroides Lm 28TONKA VASILEVA0VESELIN BIVOLARSKI1PETKO BOZOV2ILIA ILIEV3Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.Department of Biochemistry and Microbiology, Faculty of Biology, Plovdiv University, Plovdiv, Bulgaria.A study of modulation of the reactions catalyzed by dextransucrase from Leuconostoc mesenteroides Lm 28 strain in the presence of triterpenoid ursolic acid was carried out. This compound showed concentration dependent inhibition of the studied dextransucrase and Ki = 1.9 mM, which is about 5 times higher than Ki value of the known glucansucrase inhibitor acarbose. Ursolic acid affected significantly the acceptor reactions catalyzed by Lm 28 dextransucrase in the presence of maltose and sucrose to maltose ratio 2. Increasing concentrations of ursolic acid shifted concentration and degree of polymerization (DP) distribution of the synthesized glucooligosaccharides (GOS) to acceptor products with DP ≤ 5. The oligosaccharide synthesis scheme applied in this study is a promising approach for production of GOS with controlled length of the chain.http://www.jbb.uni-plovdiv.bg/documents/27807/1014561/jbb_2015-4(2)-pages_175-183.pdfacceptor reactiondextransucraseglucooligosaccharidesursolic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
TONKA VASILEVA VESELIN BIVOLARSKI PETKO BOZOV ILIA ILIEV |
spellingShingle |
TONKA VASILEVA VESELIN BIVOLARSKI PETKO BOZOV ILIA ILIEV Influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from Leuconostoc mesenteroides Lm 28 Journal of BioScience and Biotechnology acceptor reaction dextransucrase glucooligosaccharides ursolic acid |
author_facet |
TONKA VASILEVA VESELIN BIVOLARSKI PETKO BOZOV ILIA ILIEV |
author_sort |
TONKA VASILEVA |
title |
Influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from Leuconostoc mesenteroides Lm 28 |
title_short |
Influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from Leuconostoc mesenteroides Lm 28 |
title_full |
Influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from Leuconostoc mesenteroides Lm 28 |
title_fullStr |
Influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from Leuconostoc mesenteroides Lm 28 |
title_full_unstemmed |
Influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from Leuconostoc mesenteroides Lm 28 |
title_sort |
influence of ursolic acid on glucooligosaccharides synthesized by dextransucrase from leuconostoc mesenteroides lm 28 |
publisher |
Plovdiv University Press |
series |
Journal of BioScience and Biotechnology |
issn |
1314-6238 1314-6246 |
publishDate |
2015-08-01 |
description |
A study of modulation of the reactions catalyzed by dextransucrase from Leuconostoc mesenteroides Lm 28 strain in the presence of triterpenoid ursolic acid was carried out. This compound showed concentration dependent inhibition of the studied dextransucrase and Ki = 1.9 mM, which is about 5 times higher than Ki value of the known glucansucrase inhibitor acarbose. Ursolic acid affected significantly the acceptor reactions catalyzed by Lm 28 dextransucrase in the presence of maltose and sucrose to maltose ratio 2. Increasing concentrations of ursolic acid shifted concentration and degree of polymerization (DP) distribution of the synthesized glucooligosaccharides (GOS) to acceptor products with DP ≤ 5. The oligosaccharide synthesis scheme applied in this study is a promising approach for production of GOS with controlled length of the chain. |
topic |
acceptor reaction dextransucrase glucooligosaccharides ursolic acid |
url |
http://www.jbb.uni-plovdiv.bg/documents/27807/1014561/jbb_2015-4(2)-pages_175-183.pdf |
work_keys_str_mv |
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