Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers
Pharmacological concentrations of biotin have pleiotropic effects. Several reports have documented that biotin supplementation decreases hyperglycemia. We have shown that a biotin-supplemented diet increased insulin secretion and the mRNA abundance of proteins regulating insulin transcription and se...
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doaj-275e47616c704873872ebdecd3ca5f222020-11-25T00:40:39ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/72764637276463Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity MarkersLeticia Riverón-Negrete0Gloria Sicilia-Argumedo1Carolina Álvarez-Delgado2Elvia Coballase-Urrutia3Jonathan Alcántar-Fernández4Cristina Fernandez-Mejia5Unidad de Genética de la Nutrición, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Instituto Nacional de Pediatría, Avenida del Imán 1, 4th Floor, Colonia Insurgentes Cuicuilco, 04530 Mexico City, MexicoUnidad de Genética de la Nutrición, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Instituto Nacional de Pediatría, Avenida del Imán 1, 4th Floor, Colonia Insurgentes Cuicuilco, 04530 Mexico City, MexicoUnidad de Genética de la Nutrición, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Instituto Nacional de Pediatría, Avenida del Imán 1, 4th Floor, Colonia Insurgentes Cuicuilco, 04530 Mexico City, MexicoLaboratorio de Neurosciencias, Instituto Nacional de Pediatría, Avenida del Imán 1, 3rd Floor, Colonia Insurgentes Cuicuilco, 04530 Mexico City, MexicoUnidad de Genética de la Nutrición, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Instituto Nacional de Pediatría, Avenida del Imán 1, 4th Floor, Colonia Insurgentes Cuicuilco, 04530 Mexico City, MexicoUnidad de Genética de la Nutrición, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Instituto Nacional de Pediatría, Avenida del Imán 1, 4th Floor, Colonia Insurgentes Cuicuilco, 04530 Mexico City, MexicoPharmacological concentrations of biotin have pleiotropic effects. Several reports have documented that biotin supplementation decreases hyperglycemia. We have shown that a biotin-supplemented diet increased insulin secretion and the mRNA abundance of proteins regulating insulin transcription and secretion. We also found enlarged pancreatic islets and modified islet morphology. Other studies have shown that pharmacological concentrations of biotin modify tissue structure. Although biotin administration is considered safe, little attention has been given to its effect on tissue structure. In this study, we investigated the effect of biotin supplementation on hepatic morphology and liver toxicity markers. Male BALB/cAnN Hsd mice were fed a control or a biotin-supplemented diet for 8 weeks. Versus the control mice, biotin-supplemented mice had an altered portal triad with dilated sinusoids, increased vascularity, and bile conducts. Furthermore, we observed an increased proportion of nucleomegaly and binucleated hepatocytes. In spite of the liver morphological changes, no differences were observed in the serum liver damage indicators, oxidative stress markers, or antioxidant enzymes. Our data demonstrate for the first time that biotin supplementation affects liver morphology in normal mice, and that these modifications are not paralleled with damage markers.http://dx.doi.org/10.1155/2016/7276463 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leticia Riverón-Negrete Gloria Sicilia-Argumedo Carolina Álvarez-Delgado Elvia Coballase-Urrutia Jonathan Alcántar-Fernández Cristina Fernandez-Mejia |
spellingShingle |
Leticia Riverón-Negrete Gloria Sicilia-Argumedo Carolina Álvarez-Delgado Elvia Coballase-Urrutia Jonathan Alcántar-Fernández Cristina Fernandez-Mejia Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers BioMed Research International |
author_facet |
Leticia Riverón-Negrete Gloria Sicilia-Argumedo Carolina Álvarez-Delgado Elvia Coballase-Urrutia Jonathan Alcántar-Fernández Cristina Fernandez-Mejia |
author_sort |
Leticia Riverón-Negrete |
title |
Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers |
title_short |
Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers |
title_full |
Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers |
title_fullStr |
Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers |
title_full_unstemmed |
Dietary Biotin Supplementation Modifies Hepatic Morphology without Changes in Liver Toxicity Markers |
title_sort |
dietary biotin supplementation modifies hepatic morphology without changes in liver toxicity markers |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2016-01-01 |
description |
Pharmacological concentrations of biotin have pleiotropic effects. Several reports have documented that biotin supplementation decreases hyperglycemia. We have shown that a biotin-supplemented diet increased insulin secretion and the mRNA abundance of proteins regulating insulin transcription and secretion. We also found enlarged pancreatic islets and modified islet morphology. Other studies have shown that pharmacological concentrations of biotin modify tissue structure. Although biotin administration is considered safe, little attention has been given to its effect on tissue structure. In this study, we investigated the effect of biotin supplementation on hepatic morphology and liver toxicity markers. Male BALB/cAnN Hsd mice were fed a control or a biotin-supplemented diet for 8 weeks. Versus the control mice, biotin-supplemented mice had an altered portal triad with dilated sinusoids, increased vascularity, and bile conducts. Furthermore, we observed an increased proportion of nucleomegaly and binucleated hepatocytes. In spite of the liver morphological changes, no differences were observed in the serum liver damage indicators, oxidative stress markers, or antioxidant enzymes. Our data demonstrate for the first time that biotin supplementation affects liver morphology in normal mice, and that these modifications are not paralleled with damage markers. |
url |
http://dx.doi.org/10.1155/2016/7276463 |
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