Evidence for the Formation of Symmetric and Asymmetric DLPC-DAPC Lipid Bilayer Domains
Background/Aims: We investigated if mixtures of the phosphatidylcholine (PC) lipids 1,2-dilauroyl-sn-glycero-3-phosphocholine (C12:0 PC; DLPC) and 1,2-diarachidoyl-sn-glycero-3-phosphocholine (C20:0 PC; DAPC), which differ by eight methylene groups in acyl chain length, lead to the spontaneous forma...
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Cell Physiol Biochem Press GmbH & Co KG
2013-07-01
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doaj-278aa8a7b56c4374b2e8574036d304f82020-11-25T02:13:59ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-07-01321465210.1159/000350122350122Evidence for the Formation of Symmetric and Asymmetric DLPC-DAPC Lipid Bilayer DomainsMarkus RitterSabine SchmidtMartin JakabMarkus PaulmichlRobert HendersonBackground/Aims: We investigated if mixtures of the phosphatidylcholine (PC) lipids 1,2-dilauroyl-sn-glycero-3-phosphocholine (C12:0 PC; DLPC) and 1,2-diarachidoyl-sn-glycero-3-phosphocholine (C20:0 PC; DAPC), which differ by eight methylene groups in acyl chain length, lead to the spontaneous formation of distinct lipid rafts and asymmetric bilayers. Methods: The experiments were performed using Atomic Force Microscopy (AFM). Results: We show that DLPC and DAPC mixed at a molar ratio of 1:1 lead to the formation of single, double and triple bilayers with peaks at 6.14 ± 0.11, 13.27 ± 0.17 and 20.54 ± 0.46 nm, respectively (n=750). Within these formations discrete height steps of 0.92 nm can be resolved (n=422). Conclusion: The most frequently observed height steps value of 0.92 nm matches best with the calculated mean lipid hydrophobic thickness difference for asymmetric C12:0 PC and C20:0 PC lipid bilayers of 0.88 nm. This indicates the ability of DLPC and DAPC to form asymmetric lipid bilayers.http://www.karger.com/Article/FullText/350122DLPCDAPCLipid bilayerLipid domainLipid raftAtomic force microscopy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Markus Ritter Sabine Schmidt Martin Jakab Markus Paulmichl Robert Henderson |
spellingShingle |
Markus Ritter Sabine Schmidt Martin Jakab Markus Paulmichl Robert Henderson Evidence for the Formation of Symmetric and Asymmetric DLPC-DAPC Lipid Bilayer Domains Cellular Physiology and Biochemistry DLPC DAPC Lipid bilayer Lipid domain Lipid raft Atomic force microscopy |
author_facet |
Markus Ritter Sabine Schmidt Martin Jakab Markus Paulmichl Robert Henderson |
author_sort |
Markus Ritter |
title |
Evidence for the Formation of Symmetric and Asymmetric DLPC-DAPC Lipid Bilayer Domains |
title_short |
Evidence for the Formation of Symmetric and Asymmetric DLPC-DAPC Lipid Bilayer Domains |
title_full |
Evidence for the Formation of Symmetric and Asymmetric DLPC-DAPC Lipid Bilayer Domains |
title_fullStr |
Evidence for the Formation of Symmetric and Asymmetric DLPC-DAPC Lipid Bilayer Domains |
title_full_unstemmed |
Evidence for the Formation of Symmetric and Asymmetric DLPC-DAPC Lipid Bilayer Domains |
title_sort |
evidence for the formation of symmetric and asymmetric dlpc-dapc lipid bilayer domains |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2013-07-01 |
description |
Background/Aims: We investigated if mixtures of the phosphatidylcholine (PC) lipids 1,2-dilauroyl-sn-glycero-3-phosphocholine (C12:0 PC; DLPC) and 1,2-diarachidoyl-sn-glycero-3-phosphocholine (C20:0 PC; DAPC), which differ by eight methylene groups in acyl chain length, lead to the spontaneous formation of distinct lipid rafts and asymmetric bilayers. Methods: The experiments were performed using Atomic Force Microscopy (AFM). Results: We show that DLPC and DAPC mixed at a molar ratio of 1:1 lead to the formation of single, double and triple bilayers with peaks at 6.14 ± 0.11, 13.27 ± 0.17 and 20.54 ± 0.46 nm, respectively (n=750). Within these formations discrete height steps of 0.92 nm can be resolved (n=422). Conclusion: The most frequently observed height steps value of 0.92 nm matches best with the calculated mean lipid hydrophobic thickness difference for asymmetric C12:0 PC and C20:0 PC lipid bilayers of 0.88 nm. This indicates the ability of DLPC and DAPC to form asymmetric lipid bilayers. |
topic |
DLPC DAPC Lipid bilayer Lipid domain Lipid raft Atomic force microscopy |
url |
http://www.karger.com/Article/FullText/350122 |
work_keys_str_mv |
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