Isoliquiritigenin Pretreatment Induces Endoplasmic Reticulum Stress-Mediated Hormesis and Attenuates Cisplatin-Induced Oxidative Stress and Damage in LLC-PK1 Cells
Isoliquiritigenin (IsoLQ) is a flavonoid with antioxidant properties and inducer of endoplasmic reticulum (ER) stress. In vitro and in vivo studies show that ER stress-mediated hormesis is cytoprotective; therefore, natural antioxidants and ER stress inducers have been used to prevent renal injury....
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-09-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/25/19/4442 |
id |
doaj-279d4e2b26be46cba1cb7996f5248824 |
---|---|
record_format |
Article |
spelling |
doaj-279d4e2b26be46cba1cb7996f52488242020-11-25T03:47:25ZengMDPI AGMolecules1420-30492020-09-01254442444210.3390/molecules25194442Isoliquiritigenin Pretreatment Induces Endoplasmic Reticulum Stress-Mediated Hormesis and Attenuates Cisplatin-Induced Oxidative Stress and Damage in LLC-PK1 CellsTania Gómez-Sierra0Omar Noel Medina-Campos1José D. Solano2María Elena Ibarra-Rubio3José Pedraza-Chaverri4Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, CDMX 04510, MexicoDepartamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, CDMX 04510, MexicoDepartamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, CDMX 04510, MexicoDepartamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, CDMX 04510, MexicoDepartamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, CDMX 04510, MexicoIsoliquiritigenin (IsoLQ) is a flavonoid with antioxidant properties and inducer of endoplasmic reticulum (ER) stress. In vitro and in vivo studies show that ER stress-mediated hormesis is cytoprotective; therefore, natural antioxidants and ER stress inducers have been used to prevent renal injury. Oxidative stress and ER stress are some of the mechanisms of damage involved in cisplatin (CP)-induced nephrotoxicity. This study aims to explore whether IsoLQ pretreatment induces ER stress and produces hormesis to protect against CP-induced nephrotoxicity in Lilly Laboratories Cell-Porcine Kidney 1 (LLC-PK1) cells. During the first stage of this study, both IsoLQ protective concentration and pretreatment time against CP-induced toxicity were determined by cell viability. At the second stage, the effect of IsoLQ pretreatment on cell viability, ER stress, and oxidative stress were evaluated. IsoLQ pretreatment in CP-treated cells induces expression of glucose-related proteins 78 and 94 kDa (GRP78 and GRP94, respectively), attenuates CP-induced cell death, decreases reactive oxygen species (ROS) production, and prevents the decrease in glutathione/glutathione disulfide (GSH/GSSG) ratio, free thiols levels, and glutathione reductase (GR) activity. These data suggest that IsoLQ pretreatment has a moderately protective effect on CP-induced toxicity in LLC-PK1 cells, through ER stress-mediated hormesis, as well as by the antioxidant properties of IsoLQ.https://www.mdpi.com/1420-3049/25/19/4442isoliquiritigenincisplatinnephrotoxicityoxidative stressER stresshormesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tania Gómez-Sierra Omar Noel Medina-Campos José D. Solano María Elena Ibarra-Rubio José Pedraza-Chaverri |
spellingShingle |
Tania Gómez-Sierra Omar Noel Medina-Campos José D. Solano María Elena Ibarra-Rubio José Pedraza-Chaverri Isoliquiritigenin Pretreatment Induces Endoplasmic Reticulum Stress-Mediated Hormesis and Attenuates Cisplatin-Induced Oxidative Stress and Damage in LLC-PK1 Cells Molecules isoliquiritigenin cisplatin nephrotoxicity oxidative stress ER stress hormesis |
author_facet |
Tania Gómez-Sierra Omar Noel Medina-Campos José D. Solano María Elena Ibarra-Rubio José Pedraza-Chaverri |
author_sort |
Tania Gómez-Sierra |
title |
Isoliquiritigenin Pretreatment Induces Endoplasmic Reticulum Stress-Mediated Hormesis and Attenuates Cisplatin-Induced Oxidative Stress and Damage in LLC-PK1 Cells |
title_short |
Isoliquiritigenin Pretreatment Induces Endoplasmic Reticulum Stress-Mediated Hormesis and Attenuates Cisplatin-Induced Oxidative Stress and Damage in LLC-PK1 Cells |
title_full |
Isoliquiritigenin Pretreatment Induces Endoplasmic Reticulum Stress-Mediated Hormesis and Attenuates Cisplatin-Induced Oxidative Stress and Damage in LLC-PK1 Cells |
title_fullStr |
Isoliquiritigenin Pretreatment Induces Endoplasmic Reticulum Stress-Mediated Hormesis and Attenuates Cisplatin-Induced Oxidative Stress and Damage in LLC-PK1 Cells |
title_full_unstemmed |
Isoliquiritigenin Pretreatment Induces Endoplasmic Reticulum Stress-Mediated Hormesis and Attenuates Cisplatin-Induced Oxidative Stress and Damage in LLC-PK1 Cells |
title_sort |
isoliquiritigenin pretreatment induces endoplasmic reticulum stress-mediated hormesis and attenuates cisplatin-induced oxidative stress and damage in llc-pk1 cells |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-09-01 |
description |
Isoliquiritigenin (IsoLQ) is a flavonoid with antioxidant properties and inducer of endoplasmic reticulum (ER) stress. In vitro and in vivo studies show that ER stress-mediated hormesis is cytoprotective; therefore, natural antioxidants and ER stress inducers have been used to prevent renal injury. Oxidative stress and ER stress are some of the mechanisms of damage involved in cisplatin (CP)-induced nephrotoxicity. This study aims to explore whether IsoLQ pretreatment induces ER stress and produces hormesis to protect against CP-induced nephrotoxicity in Lilly Laboratories Cell-Porcine Kidney 1 (LLC-PK1) cells. During the first stage of this study, both IsoLQ protective concentration and pretreatment time against CP-induced toxicity were determined by cell viability. At the second stage, the effect of IsoLQ pretreatment on cell viability, ER stress, and oxidative stress were evaluated. IsoLQ pretreatment in CP-treated cells induces expression of glucose-related proteins 78 and 94 kDa (GRP78 and GRP94, respectively), attenuates CP-induced cell death, decreases reactive oxygen species (ROS) production, and prevents the decrease in glutathione/glutathione disulfide (GSH/GSSG) ratio, free thiols levels, and glutathione reductase (GR) activity. These data suggest that IsoLQ pretreatment has a moderately protective effect on CP-induced toxicity in LLC-PK1 cells, through ER stress-mediated hormesis, as well as by the antioxidant properties of IsoLQ. |
topic |
isoliquiritigenin cisplatin nephrotoxicity oxidative stress ER stress hormesis |
url |
https://www.mdpi.com/1420-3049/25/19/4442 |
work_keys_str_mv |
AT taniagomezsierra isoliquiritigeninpretreatmentinducesendoplasmicreticulumstressmediatedhormesisandattenuatescisplatininducedoxidativestressanddamageinllcpk1cells AT omarnoelmedinacampos isoliquiritigeninpretreatmentinducesendoplasmicreticulumstressmediatedhormesisandattenuatescisplatininducedoxidativestressanddamageinllcpk1cells AT josedsolano isoliquiritigeninpretreatmentinducesendoplasmicreticulumstressmediatedhormesisandattenuatescisplatininducedoxidativestressanddamageinllcpk1cells AT mariaelenaibarrarubio isoliquiritigeninpretreatmentinducesendoplasmicreticulumstressmediatedhormesisandattenuatescisplatininducedoxidativestressanddamageinllcpk1cells AT josepedrazachaverri isoliquiritigeninpretreatmentinducesendoplasmicreticulumstressmediatedhormesisandattenuatescisplatininducedoxidativestressanddamageinllcpk1cells |
_version_ |
1724501900436439040 |