Association of Genetic Variants in XPC and XPG Genes with Cervical Cancer Risk in a Rural Population: A Hospital Based Case Control Study

• Main Authors: Madhavi N. Patil, Kailas D. Datkhile, Pratik P. Durgawale, Nitin S. Kshirsagar, Sujata R. Kanetkar, Satish V. Kakade
• Format: Article
• Language: English
• Published: Krishna Institute of Medical Sciences University 2018-10-01
• Series: Journal of Krishna Institute of Medical Sciences University
• Subjects: Cervical cancer; Genetic polymorphism; XPC; XPG; PCR-RFLP
• Online Access: http://www.jkimsu.com/jkimsu-vol7no4/JKIMSU,%20Vol.%207,%20No.%204,%20October-December%202018%20Page%2054-64.pdf

Background: Cervical cancer is a major concern of health risk in urban and rural parts of India.. Aim and Objectives: This study was aimed to find out frequency of polymorphisms in DNA repair genes including Xeroderma pigmentosum complementation group C (XPC) and Xenoderma pigmentosum complementation group G (XPG) in patients of cervical cancer from Maharashtra and to evaluate their association with risk of cervical cancer. Materials and Methods: We used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to examine gene polymorphisms in 350 patients with cancer of cervix and 400 age and sex matched normal controls. Results: The results obtained indicated that there was no significant difference in the genotype distribution between cervical cancer patients and controls for XPC Lys939Gln, -371promoter and XPG His 1104 Asp. The result showed that genotype frequencies of XPC Val 499 Arg of codon 499 in exon 15 (OR=4.26; 95% CI=(3.007-6.03); p= <0.0001) were increased significantly. Conclusion: This study indicates that polymorphisms in Val499Arg haplotype of XPC gene appear to influence genetic susceptibility of individual to cervical cancer in Maharashtrian patients.