Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol
The association between household air pollution and lower respiratory tract infections (LRTI) among children under five years of age has been well documented; however, the extent to which the microbiome within the indoor environment contributes to this association is uncertain. The home assessment o...
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doaj-27abecb89cd945499b9d5fee33c0af132020-11-25T03:15:25ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012020-03-01176185710.3390/ijerph17061857ijerph17061857Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study ProtocolAdekunle G. Fakunle0Babatunde Olusola1Nkosana Jafta2Adedayo Faneye3Dick Heederik4Lidwien A.M. Smit5Rajen N. Naidoo6Discipline of Occupational and Environmental Health, University of KwaZulu-Natal, 321 George Campbell Building Howard College Campus, Durban 4041, South AfricaDepartment of Virology, College of Medicine, University of Ibadan, Ibadan 200212, NigeriaDiscipline of Occupational and Environmental Health, University of KwaZulu-Natal, 321 George Campbell Building Howard College Campus, Durban 4041, South AfricaDepartment of Virology, College of Medicine, University of Ibadan, Ibadan 200212, NigeriaInstitute for Risk Assessment Sciences, Environmental Epidemiology Division (IRAS-EEPI), Utrecht University, 80177 Utrecht, The NetherlandsInstitute for Risk Assessment Sciences, Environmental Epidemiology Division (IRAS-EEPI), Utrecht University, 80177 Utrecht, The NetherlandsDiscipline of Occupational and Environmental Health, University of KwaZulu-Natal, 321 George Campbell Building Howard College Campus, Durban 4041, South AfricaThe association between household air pollution and lower respiratory tract infections (LRTI) among children under five years of age has been well documented; however, the extent to which the microbiome within the indoor environment contributes to this association is uncertain. The home assessment of indoor microbiome (HAIM) study seeks to assess the abundance of indoor microbiota (IM) in the homes of under-five children (U-5Cs) with and without LRTI. HAIM is a hospital- and community-based study involving 200 cases and 200 controls recruited from three children’s hospitals in Ibadan, Nigeria. Cases will be hospital-based patients with LRTI confirmed by a pediatrician, while controls will be community-based participants, matched to cases on the basis of sex, geographical location, and age (±3 months) without LRTI. The abundance of IM in houses of cases and controls will be investigated using active and passive air sampling techniques and analyzed by qualitative detection of bacterial 16SrRNA gene (V3−V4), fungal ITS1 region, and viral RNA sequencing. HAIM is expected to elucidate the relationship between exposure to IM and incidence of LRTI among U-5Cs and ultimately provide evidence base for strategic interventions to curtail the burgeoning burden of LRTI on the subcontinent.https://www.mdpi.com/1660-4601/17/6/1857homeindoor microbiomelower respiratory tract infectionsunder-five childrenibadanstudy protocol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adekunle G. Fakunle Babatunde Olusola Nkosana Jafta Adedayo Faneye Dick Heederik Lidwien A.M. Smit Rajen N. Naidoo |
spellingShingle |
Adekunle G. Fakunle Babatunde Olusola Nkosana Jafta Adedayo Faneye Dick Heederik Lidwien A.M. Smit Rajen N. Naidoo Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol International Journal of Environmental Research and Public Health home indoor microbiome lower respiratory tract infections under-five children ibadan study protocol |
author_facet |
Adekunle G. Fakunle Babatunde Olusola Nkosana Jafta Adedayo Faneye Dick Heederik Lidwien A.M. Smit Rajen N. Naidoo |
author_sort |
Adekunle G. Fakunle |
title |
Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol |
title_short |
Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol |
title_full |
Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol |
title_fullStr |
Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol |
title_full_unstemmed |
Home Assessment of Indoor Microbiome (HAIM) in Relation to Lower Respiratory Tract Infections among Under-Five Children in Ibadan, Nigeria: The Study Protocol |
title_sort |
home assessment of indoor microbiome (haim) in relation to lower respiratory tract infections among under-five children in ibadan, nigeria: the study protocol |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2020-03-01 |
description |
The association between household air pollution and lower respiratory tract infections (LRTI) among children under five years of age has been well documented; however, the extent to which the microbiome within the indoor environment contributes to this association is uncertain. The home assessment of indoor microbiome (HAIM) study seeks to assess the abundance of indoor microbiota (IM) in the homes of under-five children (U-5Cs) with and without LRTI. HAIM is a hospital- and community-based study involving 200 cases and 200 controls recruited from three children’s hospitals in Ibadan, Nigeria. Cases will be hospital-based patients with LRTI confirmed by a pediatrician, while controls will be community-based participants, matched to cases on the basis of sex, geographical location, and age (±3 months) without LRTI. The abundance of IM in houses of cases and controls will be investigated using active and passive air sampling techniques and analyzed by qualitative detection of bacterial 16SrRNA gene (V3−V4), fungal ITS1 region, and viral RNA sequencing. HAIM is expected to elucidate the relationship between exposure to IM and incidence of LRTI among U-5Cs and ultimately provide evidence base for strategic interventions to curtail the burgeoning burden of LRTI on the subcontinent. |
topic |
home indoor microbiome lower respiratory tract infections under-five children ibadan study protocol |
url |
https://www.mdpi.com/1660-4601/17/6/1857 |
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