Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats
PURPOSE: To compare three sterilization methods (autoclave, gamma irradiation and ethylene oxide) over non demineralized lyophilized bone allografts. METHODS: Bone allografts were implanted on paravertebral muscles of 21 rats. After 30 days animals were sacrificed and grafts underwent comparative an...
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2013-01-01
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000100011 |
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doaj-27afb9fc069d436d84f400746e5d956e2020-11-24T21:09:53ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirurgica Brasileira0102-86501678-26742013-01-012816671Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in ratsOtavio Machado de AlmeidaWanda JorgettiDenis OksmanCamilo JorgettiDiógenes Laércio RochaRolf GemperliPURPOSE: To compare three sterilization methods (autoclave, gamma irradiation and ethylene oxide) over non demineralized lyophilized bone allografts. METHODS: Bone allografts were implanted on paravertebral muscles of 21 rats. After 30 days animals were sacrificed and grafts underwent comparative analysis regarding histomorphometric and macroscopic parameters. RESULTS: Allografts that underwent the three sterilization methods presents similar weight gain, cortical thickness similar to control group, and less fibrosis than the control group. Grafts that underwent sterilization in autoclave presented less presence of multinucleated giant cells, although not statistically significant. There was also no statistically significant difference regarding mineralization on the three groups. CONCLUSION: The three sterilization methods cause similar effects on bone allografts regarding macroscopic and histomorphometric parameters.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000100011Bone and BonesTransplantation, HomologousOsteogenesisSterilizationRats |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Otavio Machado de Almeida Wanda Jorgetti Denis Oksman Camilo Jorgetti Diógenes Laércio Rocha Rolf Gemperli |
spellingShingle |
Otavio Machado de Almeida Wanda Jorgetti Denis Oksman Camilo Jorgetti Diógenes Laércio Rocha Rolf Gemperli Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats Acta Cirurgica Brasileira Bone and Bones Transplantation, Homologous Osteogenesis Sterilization Rats |
author_facet |
Otavio Machado de Almeida Wanda Jorgetti Denis Oksman Camilo Jorgetti Diógenes Laércio Rocha Rolf Gemperli |
author_sort |
Otavio Machado de Almeida |
title |
Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats |
title_short |
Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats |
title_full |
Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats |
title_fullStr |
Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats |
title_full_unstemmed |
Comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats |
title_sort |
comparative study and histomorphometric analysis of bone allografts lyophilized and sterilized by autoclaving, gamma irradiation and ethylene oxide in rats |
publisher |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
series |
Acta Cirurgica Brasileira |
issn |
0102-8650 1678-2674 |
publishDate |
2013-01-01 |
description |
PURPOSE: To compare three sterilization methods (autoclave, gamma irradiation and ethylene oxide) over non demineralized lyophilized bone allografts. METHODS: Bone allografts were implanted on paravertebral muscles of 21 rats. After 30 days animals were sacrificed and grafts underwent comparative analysis regarding histomorphometric and macroscopic parameters. RESULTS: Allografts that underwent the three sterilization methods presents similar weight gain, cortical thickness similar to control group, and less fibrosis than the control group. Grafts that underwent sterilization in autoclave presented less presence of multinucleated giant cells, although not statistically significant. There was also no statistically significant difference regarding mineralization on the three groups. CONCLUSION: The three sterilization methods cause similar effects on bone allografts regarding macroscopic and histomorphometric parameters. |
topic |
Bone and Bones Transplantation, Homologous Osteogenesis Sterilization Rats |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502013000100011 |
work_keys_str_mv |
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