Intravenously Infused F3.Olig2 Improves Memory Deficits via Restoring Myelination in the Aged Hippocampus following Experimental Ischemic Stroke
Oligodendrocytes play a crucial role in creating the myelin sheath that is an important component in neural transmission. In an animal model of transient cerebral ischemia, application of oligodendrocyte progenitor cells (OPCs) has not yet been reported. In this study, the effects of F3.Olig2 transp...
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Format: | Article |
Language: | English |
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SAGE Publishing
2016-12-01
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Series: | Cell Transplantation |
Online Access: | https://doi.org/10.3727/096368916X692230 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ji Hyeon Ahn Bai Hui Chen Bich Na Shin Jeong Hwi Cho In Hye Kim Joon Ha Park Jae Chul Lee Hyun Jin Tae Yun Lyul Lee Jaesuk Lee Kyunghee Byun Goo-Bo Jeong Bonghee Lee Seung U. Kim Young-Myeong Kim Moo-Ho Won DVM, Ph.D. Soo Young Choi Ph.D. |
spellingShingle |
Ji Hyeon Ahn Bai Hui Chen Bich Na Shin Jeong Hwi Cho In Hye Kim Joon Ha Park Jae Chul Lee Hyun Jin Tae Yun Lyul Lee Jaesuk Lee Kyunghee Byun Goo-Bo Jeong Bonghee Lee Seung U. Kim Young-Myeong Kim Moo-Ho Won DVM, Ph.D. Soo Young Choi Ph.D. Intravenously Infused F3.Olig2 Improves Memory Deficits via Restoring Myelination in the Aged Hippocampus following Experimental Ischemic Stroke Cell Transplantation |
author_facet |
Ji Hyeon Ahn Bai Hui Chen Bich Na Shin Jeong Hwi Cho In Hye Kim Joon Ha Park Jae Chul Lee Hyun Jin Tae Yun Lyul Lee Jaesuk Lee Kyunghee Byun Goo-Bo Jeong Bonghee Lee Seung U. Kim Young-Myeong Kim Moo-Ho Won DVM, Ph.D. Soo Young Choi Ph.D. |
author_sort |
Ji Hyeon Ahn |
title |
Intravenously Infused F3.Olig2 Improves Memory Deficits via Restoring Myelination in the Aged Hippocampus following Experimental Ischemic Stroke |
title_short |
Intravenously Infused F3.Olig2 Improves Memory Deficits via Restoring Myelination in the Aged Hippocampus following Experimental Ischemic Stroke |
title_full |
Intravenously Infused F3.Olig2 Improves Memory Deficits via Restoring Myelination in the Aged Hippocampus following Experimental Ischemic Stroke |
title_fullStr |
Intravenously Infused F3.Olig2 Improves Memory Deficits via Restoring Myelination in the Aged Hippocampus following Experimental Ischemic Stroke |
title_full_unstemmed |
Intravenously Infused F3.Olig2 Improves Memory Deficits via Restoring Myelination in the Aged Hippocampus following Experimental Ischemic Stroke |
title_sort |
intravenously infused f3.olig2 improves memory deficits via restoring myelination in the aged hippocampus following experimental ischemic stroke |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2016-12-01 |
description |
Oligodendrocytes play a crucial role in creating the myelin sheath that is an important component in neural transmission. In an animal model of transient cerebral ischemia, application of oligodendrocyte progenitor cells (OPCs) has not yet been reported. In this study, the effects of F3.Olig2 transplantation on memory and cognitive dysfunction were investigated in the aged gerbil in which ischemic stroke was induced. To investigate the possible mechanisms underlying repair, changes in the expression of myelin basic protein (MBP), oligodendrocyte-specific protein (OSP), and brain-derived neurotrophic factor (BDNF) were examined. Experimental ischemic stroke was induced by occlusion of bilateral common carotid arteries in aged gerbils. Gerbils ( n = 31 per group) were randomly divided into three groups: (1) vehicle sham group, (2) vehicle ischemia group, and (3) F3.Olig2 ischemia group. After 1, 3, and 7 days of ischemia–reperfusion (I-R), saline or F3.Olig2 cells (1 × 10 6 cells in 100 μl) were injected into the gerbils intravenously. The gerbils were sacrificed 10 days after I-R for identification of grafted F3.Olig2 cells, and 15 and 30 days after I-R for tissue analysis after conducting passive avoidance and novel object recognition test. Injected F3.Olig2 cells and MBP, OSP, and BDNF were detected by specific antibodies using immunohistochemistry and/or Western blots. Memory and cognition were significantly increased in the F3.Olig2 ischemia group compared with the vehicle ischemia group. In the F3.Olig2 ischemia group, the neurons were not protected from ischemic damage; however, MBP, OSP, and BDNF expressions were significantly increased. Our results show that injection of F3.Olig2 cells significantly improved impaired memory and cognition, which might be related to increased MBP expression via increasing OSP and BDNF expression in the aged gerbil hippocampus following transient cerebral ischemia. |
url |
https://doi.org/10.3727/096368916X692230 |
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doaj-27c5e5ced30e4d73baf30a5c5aac41122020-11-25T03:44:01ZengSAGE PublishingCell Transplantation0963-68971555-38922016-12-012510.3727/096368916X692230Intravenously Infused F3.Olig2 Improves Memory Deficits via Restoring Myelination in the Aged Hippocampus following Experimental Ischemic StrokeJi Hyeon Ahn0Bai Hui Chen1Bich Na Shin2Jeong Hwi Cho3In Hye Kim4Joon Ha Park5Jae Chul Lee6Hyun Jin Tae7Yun Lyul Lee8Jaesuk Lee9Kyunghee Byun10Goo-Bo Jeong11Bonghee Lee12Seung U. Kim13Young-Myeong Kim14Moo-Ho Won DVM, Ph.D.15Soo Young Choi Ph.D.16Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, South KoreaDepartment of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang, P.R. ChinaDepartment of Physiology, College of Medicine, Institute of Neurodegeneration and Neuroregeneration, Hallym University, Chuncheon, South KoreaDepartment of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South KoreaDepartment of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South KoreaDepartment of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, South KoreaDepartment of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South KoreaBio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, Iksan, South KoreaDepartment of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang, P.R. ChinaCenter for Genomics and Proteomics, Institute for Regenerative Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, South KoreaDepartment of Anatomy and Cell Biology, Gachon University Graduate School of Medicine, Incheon, South KoreaDepartment of Anatomy and Cell Biology, Gachon University Graduate School of Medicine, Incheon, South KoreaDepartment of Anatomy and Cell Biology, Gachon University Graduate School of Medicine, Incheon, South KoreaDepartment of Medicine, University of British Columbia, Vancouver, British Columbia, CanadaDepartment of Molecular and Cellular Biochemistry, School of Medicine, Kangwon National University, Chuncheon, South KoreaDepartment of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South KoreaDepartment of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, South KoreaOligodendrocytes play a crucial role in creating the myelin sheath that is an important component in neural transmission. In an animal model of transient cerebral ischemia, application of oligodendrocyte progenitor cells (OPCs) has not yet been reported. In this study, the effects of F3.Olig2 transplantation on memory and cognitive dysfunction were investigated in the aged gerbil in which ischemic stroke was induced. To investigate the possible mechanisms underlying repair, changes in the expression of myelin basic protein (MBP), oligodendrocyte-specific protein (OSP), and brain-derived neurotrophic factor (BDNF) were examined. Experimental ischemic stroke was induced by occlusion of bilateral common carotid arteries in aged gerbils. Gerbils ( n = 31 per group) were randomly divided into three groups: (1) vehicle sham group, (2) vehicle ischemia group, and (3) F3.Olig2 ischemia group. After 1, 3, and 7 days of ischemia–reperfusion (I-R), saline or F3.Olig2 cells (1 × 10 6 cells in 100 μl) were injected into the gerbils intravenously. The gerbils were sacrificed 10 days after I-R for identification of grafted F3.Olig2 cells, and 15 and 30 days after I-R for tissue analysis after conducting passive avoidance and novel object recognition test. Injected F3.Olig2 cells and MBP, OSP, and BDNF were detected by specific antibodies using immunohistochemistry and/or Western blots. Memory and cognition were significantly increased in the F3.Olig2 ischemia group compared with the vehicle ischemia group. In the F3.Olig2 ischemia group, the neurons were not protected from ischemic damage; however, MBP, OSP, and BDNF expressions were significantly increased. Our results show that injection of F3.Olig2 cells significantly improved impaired memory and cognition, which might be related to increased MBP expression via increasing OSP and BDNF expression in the aged gerbil hippocampus following transient cerebral ischemia.https://doi.org/10.3727/096368916X692230 |