Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology.
We investigated potential protein markers of post-mortem interval (PMI) using rat kidney and psoas muscle. Tissue samples were taken at 12 h intervals for up to 96 h after death by suffocation. Expression levels of eight soluble proteins were analyzed by Western blotting. Degradation patterns of sel...
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doaj-27de4ac51b154d9f83a6cef18a299b462020-11-25T01:30:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01118e016055710.1371/journal.pone.0160557Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology.Dong-Gi LeeKyeong Eun YangJeong Won HwangHwan-Soo KangSeung-Yeul LeeSeoyeon ChoiJoonchul ShinIk-Soon JangHyun Joo AnHeesun ChungHyo-Il JungJong-Soon ChoiWe investigated potential protein markers of post-mortem interval (PMI) using rat kidney and psoas muscle. Tissue samples were taken at 12 h intervals for up to 96 h after death by suffocation. Expression levels of eight soluble proteins were analyzed by Western blotting. Degradation patterns of selected proteins were clearly divided into three groups: short-term, mid-term, and long-term PMI markers based on the half maximum intensity of intact protein expression. In kidney, glycogen synthase (GS) and glycogen synthase kinase-3β were degraded completely within 48 h making them short-term PMI markers. AMP-activated protein kinase α, caspase 3 and GS were short-term PMI markers in psoas muscle. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was a mid-term PMI marker in both tissues. Expression levels of the typical long-term PMI markers, p53 and β-catenin, were constant for at least 96 h post-mortem in both tissues. The degradation patterns of GS and caspase-3 were verified by immunohistochemistry in both tissues. GAPDH was chosen as a test PMI protein to perform a lateral flow assay (LFA). The presence of recombinant GAPDH was clearly detected in LFA and quantified in a concentration-dependent manner. These results suggest that LFA might be used to estimate PMI at a crime scene.http://europepmc.org/articles/PMC4995019?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dong-Gi Lee Kyeong Eun Yang Jeong Won Hwang Hwan-Soo Kang Seung-Yeul Lee Seoyeon Choi Joonchul Shin Ik-Soon Jang Hyun Joo An Heesun Chung Hyo-Il Jung Jong-Soon Choi |
spellingShingle |
Dong-Gi Lee Kyeong Eun Yang Jeong Won Hwang Hwan-Soo Kang Seung-Yeul Lee Seoyeon Choi Joonchul Shin Ik-Soon Jang Hyun Joo An Heesun Chung Hyo-Il Jung Jong-Soon Choi Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology. PLoS ONE |
author_facet |
Dong-Gi Lee Kyeong Eun Yang Jeong Won Hwang Hwan-Soo Kang Seung-Yeul Lee Seoyeon Choi Joonchul Shin Ik-Soon Jang Hyun Joo An Heesun Chung Hyo-Il Jung Jong-Soon Choi |
author_sort |
Dong-Gi Lee |
title |
Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology. |
title_short |
Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology. |
title_full |
Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology. |
title_fullStr |
Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology. |
title_full_unstemmed |
Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology. |
title_sort |
degradation of kidney and psoas muscle proteins as indicators of post-mortem interval in a rat model, with use of lateral flow technology. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
We investigated potential protein markers of post-mortem interval (PMI) using rat kidney and psoas muscle. Tissue samples were taken at 12 h intervals for up to 96 h after death by suffocation. Expression levels of eight soluble proteins were analyzed by Western blotting. Degradation patterns of selected proteins were clearly divided into three groups: short-term, mid-term, and long-term PMI markers based on the half maximum intensity of intact protein expression. In kidney, glycogen synthase (GS) and glycogen synthase kinase-3β were degraded completely within 48 h making them short-term PMI markers. AMP-activated protein kinase α, caspase 3 and GS were short-term PMI markers in psoas muscle. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was a mid-term PMI marker in both tissues. Expression levels of the typical long-term PMI markers, p53 and β-catenin, were constant for at least 96 h post-mortem in both tissues. The degradation patterns of GS and caspase-3 were verified by immunohistochemistry in both tissues. GAPDH was chosen as a test PMI protein to perform a lateral flow assay (LFA). The presence of recombinant GAPDH was clearly detected in LFA and quantified in a concentration-dependent manner. These results suggest that LFA might be used to estimate PMI at a crime scene. |
url |
http://europepmc.org/articles/PMC4995019?pdf=render |
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