Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605
Virulence factor regulator (Vfr) is an indispensable transcription factor in the expression of virulence in the phytopathogenic bacteria Pseudomonas syringae. However, the function of Vfr is not known so far. The deletion of vfr resulted in the loss of surface swarming motility and reduced the virul...
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doaj-27e6225c4eda4c26a473cd47c9b5098f2021-06-05T06:09:42ZengElsevierBiochemistry and Biophysics Reports2405-58082021-07-0126100944Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605Keisuke Ogura0Hidenori Matsui1Mikihiro Yamamoto2Yoshiteru Noutoshi3Kazuhiro Toyoda4Fumiko Taguchi5Yuki Ichinose6Graduate School of Environmental and Life Science, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, JapanGraduate School of Environmental and Life Science, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, Japan; Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, JapanGraduate School of Environmental and Life Science, Okayama University, Tsushima-naka 1-1-1, Kita-ku, Okayama, 700-8530, Japan; Corresponding author.Virulence factor regulator (Vfr) is an indispensable transcription factor in the expression of virulence in the phytopathogenic bacteria Pseudomonas syringae. However, the function of Vfr is not known so far. The deletion of vfr resulted in the loss of surface swarming motility and reduced the virulence in P. syringae pv. tabaci (Pta) 6605. In order to identify the target genes of Vfr, we screened the sequences that bind to Vfr by chromatin immune precipitation (ChIP) and sequencing methods using the closely related bacterium P. syringae pv. syringae (Pss) B728a. For this purpose we first generated a strain that possesses the recombinant gene vfr::FLAG in Pss B728a, and performed ChIP using an anti-FLAG antibody. Immunoprecipitated DNA was purified and sequenced with Illumina HiSeq. The Vfr::FLAG-specific peaks were further subjected to an electrophoresis mobility-shift assay, and the promoter regions of locus tag for Psyr_0578 , Psyr_1776, and Psyr_2237 were identified as putative target genes of Vfr. These genes encode plant pathogen–specific methyl-accepting chemotaxis proteins (Mcp). These mcp genes seem to be involved in the Vfr-regulated expression of virulence.http://www.sciencedirect.com/science/article/pii/S2405580821000388chemotaxischromatin immunoprecipitation (ChIP)ChIP-seqMethyl-accepting chemotaxis protein (Mcp)Transcriptional regulationVirulence factor regulator (Vfr) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Keisuke Ogura Hidenori Matsui Mikihiro Yamamoto Yoshiteru Noutoshi Kazuhiro Toyoda Fumiko Taguchi Yuki Ichinose |
spellingShingle |
Keisuke Ogura Hidenori Matsui Mikihiro Yamamoto Yoshiteru Noutoshi Kazuhiro Toyoda Fumiko Taguchi Yuki Ichinose Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605 Biochemistry and Biophysics Reports chemotaxis chromatin immunoprecipitation (ChIP) ChIP-seq Methyl-accepting chemotaxis protein (Mcp) Transcriptional regulation Virulence factor regulator (Vfr) |
author_facet |
Keisuke Ogura Hidenori Matsui Mikihiro Yamamoto Yoshiteru Noutoshi Kazuhiro Toyoda Fumiko Taguchi Yuki Ichinose |
author_sort |
Keisuke Ogura |
title |
Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605 |
title_short |
Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605 |
title_full |
Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605 |
title_fullStr |
Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605 |
title_full_unstemmed |
Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605 |
title_sort |
vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in pseudomonas syringae pv. tabaci 6605 |
publisher |
Elsevier |
series |
Biochemistry and Biophysics Reports |
issn |
2405-5808 |
publishDate |
2021-07-01 |
description |
Virulence factor regulator (Vfr) is an indispensable transcription factor in the expression of virulence in the phytopathogenic bacteria Pseudomonas syringae. However, the function of Vfr is not known so far. The deletion of vfr resulted in the loss of surface swarming motility and reduced the virulence in P. syringae pv. tabaci (Pta) 6605. In order to identify the target genes of Vfr, we screened the sequences that bind to Vfr by chromatin immune precipitation (ChIP) and sequencing methods using the closely related bacterium P. syringae pv. syringae (Pss) B728a. For this purpose we first generated a strain that possesses the recombinant gene vfr::FLAG in Pss B728a, and performed ChIP using an anti-FLAG antibody. Immunoprecipitated DNA was purified and sequenced with Illumina HiSeq. The Vfr::FLAG-specific peaks were further subjected to an electrophoresis mobility-shift assay, and the promoter regions of locus tag for Psyr_0578 , Psyr_1776, and Psyr_2237 were identified as putative target genes of Vfr. These genes encode plant pathogen–specific methyl-accepting chemotaxis proteins (Mcp). These mcp genes seem to be involved in the Vfr-regulated expression of virulence. |
topic |
chemotaxis chromatin immunoprecipitation (ChIP) ChIP-seq Methyl-accepting chemotaxis protein (Mcp) Transcriptional regulation Virulence factor regulator (Vfr) |
url |
http://www.sciencedirect.com/science/article/pii/S2405580821000388 |
work_keys_str_mv |
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