Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India

Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India

Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-...

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Main Authors: Aman Kamboj, Atul Kumar Pateriya, Anamika Mishra, Pradip Ranaware, Diwakar D. Kulkarni, Ashwin Ashok Raut
Format: Article
Language:English
Published: Hindawi Limited 2014-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2014/496219
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spelling doaj-2808713eebd14fa3861767c6515364c82020-11-25T00:08:06ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/496219496219Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in IndiaAman Kamboj0Atul Kumar Pateriya1Anamika Mishra2Pradip Ranaware3Diwakar D. Kulkarni4Ashwin Ashok Raut5High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, IndiaHigh Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, IndiaHigh Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, IndiaHigh Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, IndiaHigh Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, IndiaHigh Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal 462022, IndiaCrimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-based real-time RT-PCR assay for rapid, sensitive, and specific diagnosis of Crimean-Congo hemorrhagic fever virus (CCHFV). The developed assay was found to be a better alternative to the reported TaqMan assay for routine diagnosis of CCHF.http://dx.doi.org/10.1155/2014/496219
collection DOAJ
language English
format Article
sources DOAJ
author Aman Kamboj
Atul Kumar Pateriya
Anamika Mishra
Pradip Ranaware
Diwakar D. Kulkarni
Ashwin Ashok Raut
spellingShingle Aman Kamboj
Atul Kumar Pateriya
Anamika Mishra
Pradip Ranaware
Diwakar D. Kulkarni
Ashwin Ashok Raut
Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
BioMed Research International
author_facet Aman Kamboj
Atul Kumar Pateriya
Anamika Mishra
Pradip Ranaware
Diwakar D. Kulkarni
Ashwin Ashok Raut
author_sort Aman Kamboj
title Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_short Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_full Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_fullStr Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_full_unstemmed Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
title_sort novel molecular beacon probe-based real-time rt-pcr assay for diagnosis of crimean-congo hemorrhagic fever encountered in india
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2014-01-01
description Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-based real-time RT-PCR assay for rapid, sensitive, and specific diagnosis of Crimean-Congo hemorrhagic fever virus (CCHFV). The developed assay was found to be a better alternative to the reported TaqMan assay for routine diagnosis of CCHF.
url http://dx.doi.org/10.1155/2014/496219
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