Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell Lines

Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell Lines

Tualang honey (TH) is rich in flavonoids and phenolic acids and has significant anticancer activity against breast cancer cells comparable to the effect of tamoxifen (TAM), in vitro. The current study evaluated the effects of TH when used in combination with TAM on MCF-7 and MDA-MB-231 cells. We obs...

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Main Authors: Nik Soriani Yaacob, Agustine Nengsih, Mohd. Nor Norazmi
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:Evidence-Based Complementary and Alternative Medicine
Online Access:http://dx.doi.org/10.1155/2013/989841
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spelling doaj-28111e7f154a4be482a71764220becc92020-11-24T20:53:04ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882013-01-01201310.1155/2013/989841989841Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell LinesNik Soriani Yaacob0Agustine Nengsih1Mohd. Nor Norazmi2Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Malaysia Health Campus, 16150 Kubang Kerian, Kelantan, MalaysiaDepartment of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Malaysia Health Campus, 16150 Kubang Kerian, Kelantan, MalaysiaSchool of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, MalaysiaTualang honey (TH) is rich in flavonoids and phenolic acids and has significant anticancer activity against breast cancer cells comparable to the effect of tamoxifen (TAM), in vitro. The current study evaluated the effects of TH when used in combination with TAM on MCF-7 and MDA-MB-231 cells. We observed that TH promoted the anticancer activity of TAM in both the estrogen receptor-(ER-)responsive and ER-nonresponsive human breast cancer cell lines. Flow cytometric analyses indicated accelerated apoptosis especially in MDA-MB-231 cells and with the involvement of caspase-3/7, -8 and -9 activation as shown by fluorescence microscopy. Depolarization of the mitochondrial membrane was also increased in both cell lines when TH was used in combination with TAM compared to TAM treatment alone. TH may therefore be a potential adjuvant to be used with TAM for reducing the dose of TAM, hence, reducing TAM-induced adverse effects.http://dx.doi.org/10.1155/2013/989841
collection DOAJ
language English
format Article
sources DOAJ
author Nik Soriani Yaacob
Agustine Nengsih
Mohd. Nor Norazmi
spellingShingle Nik Soriani Yaacob
Agustine Nengsih
Mohd. Nor Norazmi
Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell Lines
Evidence-Based Complementary and Alternative Medicine
author_facet Nik Soriani Yaacob
Agustine Nengsih
Mohd. Nor Norazmi
author_sort Nik Soriani Yaacob
title Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell Lines
title_short Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell Lines
title_full Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell Lines
title_fullStr Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell Lines
title_full_unstemmed Tualang Honey Promotes Apoptotic Cell Death Induced by Tamoxifen in Breast Cancer Cell Lines
title_sort tualang honey promotes apoptotic cell death induced by tamoxifen in breast cancer cell lines
publisher Hindawi Limited
series Evidence-Based Complementary and Alternative Medicine
issn 1741-427X
1741-4288
publishDate 2013-01-01
description Tualang honey (TH) is rich in flavonoids and phenolic acids and has significant anticancer activity against breast cancer cells comparable to the effect of tamoxifen (TAM), in vitro. The current study evaluated the effects of TH when used in combination with TAM on MCF-7 and MDA-MB-231 cells. We observed that TH promoted the anticancer activity of TAM in both the estrogen receptor-(ER-)responsive and ER-nonresponsive human breast cancer cell lines. Flow cytometric analyses indicated accelerated apoptosis especially in MDA-MB-231 cells and with the involvement of caspase-3/7, -8 and -9 activation as shown by fluorescence microscopy. Depolarization of the mitochondrial membrane was also increased in both cell lines when TH was used in combination with TAM compared to TAM treatment alone. TH may therefore be a potential adjuvant to be used with TAM for reducing the dose of TAM, hence, reducing TAM-induced adverse effects.
url http://dx.doi.org/10.1155/2013/989841
work_keys_str_mv AT niksorianiyaacob tualanghoneypromotesapoptoticcelldeathinducedbytamoxifeninbreastcancercelllines
AT agustinenengsih tualanghoneypromotesapoptoticcelldeathinducedbytamoxifeninbreastcancercelllines
AT mohdnornorazmi tualanghoneypromotesapoptoticcelldeathinducedbytamoxifeninbreastcancercelllines
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