Comprehensive Analysis of Porcine <em>Prox1</em> Gene and Its Relationship with Meat Quality Traits
<i>Prox1</i> is involved in muscle fiber conversion, adult-onset obesity, and type 2 diabetes. However, information regarding porcine <i>Prox1</i> and its relationship with meat quality traits is still unknown. In this study, we characterized the full-length cDNA and proximal...
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doaj-28174daa429f4b3d8d73ad951d859d192020-11-25T02:36:22ZengMDPI AGAnimals2076-26152019-09-0191074410.3390/ani9100744ani9100744Comprehensive Analysis of Porcine <em>Prox1</em> Gene and Its Relationship with Meat Quality TraitsChao Dong0Xiying Zhang1Kaiqing Liu2Bojiang Li3Zhe Chao4Aiwen Jiang5Rongyang Li6Pinghua Li7Honglin Liu8Wangjun Wu9Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Animal Sciences & Veterinary, Hainan Academy of Agricultural Sciences, Haikou 571100, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDepartment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China<i>Prox1</i> is involved in muscle fiber conversion, adult-onset obesity, and type 2 diabetes. However, information regarding porcine <i>Prox1</i> and its relationship with meat quality traits is still unknown. In this study, we characterized the full-length cDNA and proximal promoter of two transcript variants of porcine <i>Prox1</i>. Moreover, <i>Prox1</i> was expressed abundantly in the skeletal muscle and its expression was higher in the soleus muscle than that in the biceps femoris muscle. Its expression pattern in the high and low meat color (redness) value <i>a</i>* groups was similar to that of <i>myoglobin</i> and <i>MyHC I</i>, but opposed to that of <i>MyHC IIB</i>. Importantly, there was a significant positive correlation between <i>Prox1</i> expression and meat color (redness) value <i>a</i>* (r = 0.3845, <i>p</i> = 0.0394), and a significant negative correlation between <i>Prox1</i> expression and drip loss (r = −0.4204, <i>p</i> = 0.0232), as well as the ratio of <i>MyHC IIB</i> to <i>MyHC I</i> expression (r = −0.3871, <i>p</i> = 0.0380). In addition, we found that the polymorphisms of three closely linked SNPs in <i>Prox1</i> promoter 1 were significantly associated with pH<sub>24h</sub> in a pig population. Taken together, our data provide valuable insights into the characteristics of porcine <i>Prox1</i> and indicate that <i>Prox1</i> is a promising candidate gene affecting meat quality traits.https://www.mdpi.com/2076-2615/9/10/744pigmeat quality<i>prox1</i>variationassociation analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chao Dong Xiying Zhang Kaiqing Liu Bojiang Li Zhe Chao Aiwen Jiang Rongyang Li Pinghua Li Honglin Liu Wangjun Wu |
spellingShingle |
Chao Dong Xiying Zhang Kaiqing Liu Bojiang Li Zhe Chao Aiwen Jiang Rongyang Li Pinghua Li Honglin Liu Wangjun Wu Comprehensive Analysis of Porcine <em>Prox1</em> Gene and Its Relationship with Meat Quality Traits Animals pig meat quality <i>prox1</i> variation association analysis |
author_facet |
Chao Dong Xiying Zhang Kaiqing Liu Bojiang Li Zhe Chao Aiwen Jiang Rongyang Li Pinghua Li Honglin Liu Wangjun Wu |
author_sort |
Chao Dong |
title |
Comprehensive Analysis of Porcine <em>Prox1</em> Gene and Its Relationship with Meat Quality Traits |
title_short |
Comprehensive Analysis of Porcine <em>Prox1</em> Gene and Its Relationship with Meat Quality Traits |
title_full |
Comprehensive Analysis of Porcine <em>Prox1</em> Gene and Its Relationship with Meat Quality Traits |
title_fullStr |
Comprehensive Analysis of Porcine <em>Prox1</em> Gene and Its Relationship with Meat Quality Traits |
title_full_unstemmed |
Comprehensive Analysis of Porcine <em>Prox1</em> Gene and Its Relationship with Meat Quality Traits |
title_sort |
comprehensive analysis of porcine <em>prox1</em> gene and its relationship with meat quality traits |
publisher |
MDPI AG |
series |
Animals |
issn |
2076-2615 |
publishDate |
2019-09-01 |
description |
<i>Prox1</i> is involved in muscle fiber conversion, adult-onset obesity, and type 2 diabetes. However, information regarding porcine <i>Prox1</i> and its relationship with meat quality traits is still unknown. In this study, we characterized the full-length cDNA and proximal promoter of two transcript variants of porcine <i>Prox1</i>. Moreover, <i>Prox1</i> was expressed abundantly in the skeletal muscle and its expression was higher in the soleus muscle than that in the biceps femoris muscle. Its expression pattern in the high and low meat color (redness) value <i>a</i>* groups was similar to that of <i>myoglobin</i> and <i>MyHC I</i>, but opposed to that of <i>MyHC IIB</i>. Importantly, there was a significant positive correlation between <i>Prox1</i> expression and meat color (redness) value <i>a</i>* (r = 0.3845, <i>p</i> = 0.0394), and a significant negative correlation between <i>Prox1</i> expression and drip loss (r = −0.4204, <i>p</i> = 0.0232), as well as the ratio of <i>MyHC IIB</i> to <i>MyHC I</i> expression (r = −0.3871, <i>p</i> = 0.0380). In addition, we found that the polymorphisms of three closely linked SNPs in <i>Prox1</i> promoter 1 were significantly associated with pH<sub>24h</sub> in a pig population. Taken together, our data provide valuable insights into the characteristics of porcine <i>Prox1</i> and indicate that <i>Prox1</i> is a promising candidate gene affecting meat quality traits. |
topic |
pig meat quality <i>prox1</i> variation association analysis |
url |
https://www.mdpi.com/2076-2615/9/10/744 |
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