The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis
Toonaciliatin K is a natural limonoid purified from the Toona ciliata Roem. var. ciliata (Meliaceae). This study is to reveal the inflammatory suppression effect of toonaciliatin K and further the intrinsic mechanism. Firstly, anti-inflammatory effect of toonaciliatin K was evaluated in lipopolysacc...
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Online Access: | http://dx.doi.org/10.1155/2017/9436280 |
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doaj-2818018d79b245a38e954f75f0936e9c2020-11-24T22:49:32ZengHindawi LimitedBioMed Research International2314-61332314-61412017-01-01201710.1155/2017/94362809436280The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant ArthritisHaiXing Gou0Jie Ye1YiRu Wang2XiaoLi Xu3QiXing Shen4JingWei Xue5Jie Zhao6XinGang Lu7Department of Traditional Chinese Medicine, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital, Fudan University, Shanghai 200040, ChinaDepartment of Orthopaedics, Longhua Hospital, Shanghai Traditional Chinese Medicine University, Shanghai 200032, ChinaDepartment of Orthopaedics, Longhua Hospital, Shanghai Traditional Chinese Medicine University, Shanghai 200032, ChinaDepartment of Traditional Chinese Medicine, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital, Fudan University, Shanghai 200040, ChinaDepartment of Orthopaedics, Longhua Hospital, Shanghai Traditional Chinese Medicine University, Shanghai 200032, ChinaDepartment of Emergency, Jiading Hospital of Traditional Chinese Medicine, Shanghai 201899, ChinaDepartment of Traditional Chinese Medicine, Sixth People’s Hospital, Jinshan Branch, Shanghai 201500, ChinaDepartment of Traditional Chinese Medicine, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital, Fudan University, Shanghai 200040, ChinaToonaciliatin K is a natural limonoid purified from the Toona ciliata Roem. var. ciliata (Meliaceae). This study is to reveal the inflammatory suppression effect of toonaciliatin K and further the intrinsic mechanism. Firstly, anti-inflammatory effect of toonaciliatin K was evaluated in lipopolysaccharide- (LPS-) induced RAW264.7 cells. RT-PCR results indicated that the mRNA expressions of TNF-α, IL-6, and IL-1β were downregulated by toonaciliatin K. The toonaciliatin K inhibited TNF-α, IL-6, and IL-1β levels stimulated by LPS. Furthermore, LPS elicited the excess iNOS and COX-2 mRNA and protein production and toonaciliatin K attenuated the excess production. Western blot assay demonstrated that MAPK and NF-κB signaling pathways play critical roles in the toonaciliatin K’s anti-inflammatory activity. Secondly, toonaciliatin K inhibited carrageenan-induced paw edema in rats. Thirdly, toonaciliatin K alleviated the paw swelling and improved arthritis clinical scores in the adjuvant arthritis rats. Toonaciliatin K decreased the proinflammatory cytokines levels and Mankin scores in adjuvant arthritis rats. The HE staining, safranin O-fast green, and toluidine blue staining results demonstrated that toonaciliatin K alleviated the histological changes of paw, for example, pannus formation, focal loss of cartilage, bone erosion, and presence of extra-articular inflammation. Hence, toonaciliatin K is a promising agent for treatment of arthritis.http://dx.doi.org/10.1155/2017/9436280 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
HaiXing Gou Jie Ye YiRu Wang XiaoLi Xu QiXing Shen JingWei Xue Jie Zhao XinGang Lu |
spellingShingle |
HaiXing Gou Jie Ye YiRu Wang XiaoLi Xu QiXing Shen JingWei Xue Jie Zhao XinGang Lu The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis BioMed Research International |
author_facet |
HaiXing Gou Jie Ye YiRu Wang XiaoLi Xu QiXing Shen JingWei Xue Jie Zhao XinGang Lu |
author_sort |
HaiXing Gou |
title |
The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis |
title_short |
The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis |
title_full |
The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis |
title_fullStr |
The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis |
title_full_unstemmed |
The Anti-Inflammatory Activity of Toonaciliatin K against Adjuvant Arthritis |
title_sort |
anti-inflammatory activity of toonaciliatin k against adjuvant arthritis |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2017-01-01 |
description |
Toonaciliatin K is a natural limonoid purified from the Toona ciliata Roem. var. ciliata (Meliaceae). This study is to reveal the inflammatory suppression effect of toonaciliatin K and further the intrinsic mechanism. Firstly, anti-inflammatory effect of toonaciliatin K was evaluated in lipopolysaccharide- (LPS-) induced RAW264.7 cells. RT-PCR results indicated that the mRNA expressions of TNF-α, IL-6, and IL-1β were downregulated by toonaciliatin K. The toonaciliatin K inhibited TNF-α, IL-6, and IL-1β levels stimulated by LPS. Furthermore, LPS elicited the excess iNOS and COX-2 mRNA and protein production and toonaciliatin K attenuated the excess production. Western blot assay demonstrated that MAPK and NF-κB signaling pathways play critical roles in the toonaciliatin K’s anti-inflammatory activity. Secondly, toonaciliatin K inhibited carrageenan-induced paw edema in rats. Thirdly, toonaciliatin K alleviated the paw swelling and improved arthritis clinical scores in the adjuvant arthritis rats. Toonaciliatin K decreased the proinflammatory cytokines levels and Mankin scores in adjuvant arthritis rats. The HE staining, safranin O-fast green, and toluidine blue staining results demonstrated that toonaciliatin K alleviated the histological changes of paw, for example, pannus formation, focal loss of cartilage, bone erosion, and presence of extra-articular inflammation. Hence, toonaciliatin K is a promising agent for treatment of arthritis. |
url |
http://dx.doi.org/10.1155/2017/9436280 |
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