A Comparative Study of Histopathology of Astrocytomas with Intraoperative Cytology with Special Reference to MIB-1 Labelling Index
Background: Although radiological imaging and surgical techniques have substantially improved, assessment of prognosis by grading astrocytoma has been challenging task for pathologists. We have tried to assess these tumours intraoperatively for rapid diagnosis. Also MIB-1Labelling index (MIB-1 LI...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2015-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/6281/12372_CE(RA1)_F(T)_PF1(ANAK)_PFA(P)_PF2(PAG).pdf |
| Summary: | Background: Although radiological imaging and surgical techniques have substantially improved, assessment of prognosis by
grading astrocytoma has been challenging task for pathologists.
We have tried to assess these tumours intraoperatively for rapid
diagnosis. Also MIB-1Labelling index (MIB-1 LI) was done to study
their proliferative activity.
Aims: To compare and grade squash smear technique with histopathology to study its utility in diagnosis of astrocytomas. Further, to
study correlation of various grades with their MIB-1 labelling index.
Setting and Designs: This study was carried out in the Department
of Pathology, SMS Medical College, Jaipur over a period of one
year.
Materials and Methods: Forty five cases of radiologically
suspected astrocytomas were submitted for intraoperative cytology
and later for histopathology. Two techniques were compared for
ability to diagnose as well as grade the tumour. Also, MIB-1 LI were
performed in biopsy tissue. Histopathological and immunological
grades were compared.
Results: Out of 45 cases 44(97.7%) correctly diagnosed by
squash smear technique. Further significant differences seen in
values of MIB-1 LI of high-grade and low-grade astrocytomas. A
progressive increase in the MIB-1 LI was observed with increasing
grades. In grade I astrocytoma MIB-1 LI was <0.05%. In grade
II astrocytoma it varied from 0.8-2.6% except in one case where
it was 3.2% who presented with a recurrent mass. In grade III,
MIB-1 LI was 3.5% to 7.5%. In grade IV was 10-20%.
Conclusion: Intraoperative cytology is fairly accurate and useful in
intraoperative consultation. Also, MIB-1 LI can be a useful adjunct
for grading particularly in small biopsies. |
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| ISSN: | 2249-782X 0973-709X |