Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1

• Main Authors: Zhikui Hao, Yujie Cai, Xiangru Liao, Xiaoli Zhang, Zhiyou Fang, Dabing Zhang
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Microbiologia 2012-03-01
• Series: Brazilian Journal of Microbiology
• Subjects: chitinase; medium optimization; response surface method; inulin; Chitinolyticbacter meiyuanensis SYBC-H1
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100019

The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.