The Oral Microbiota May Have Influence on Oral Cancer
The oral microbiota plays an important role in the human microbiome and human health, and imbalances between microbes and their hosts can lead to oral and systemic diseases and chronic inflammation, which is usually caused by bacteria and contributes to cancer. There may be a relationship between or...
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doaj-28599d80c1454bbe9e89fa900f94741e2020-11-25T02:25:57ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-01-01910.3389/fcimb.2019.00476493614The Oral Microbiota May Have Influence on Oral CancerLing Zhang0Ling Zhang1Ling Zhang2Yuan Liu3Hua Jun Zheng4Chen Ping Zhang5Department of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Clinical Research Center for Oral Diseases, Shanghai, ChinaShanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, ChinaDepartment of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Fudan University, Shanghai, ChinaDepartment of Oral and Maxillofacial-Head and Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaThe oral microbiota plays an important role in the human microbiome and human health, and imbalances between microbes and their hosts can lead to oral and systemic diseases and chronic inflammation, which is usually caused by bacteria and contributes to cancer. There may be a relationship between oral bacteria and oral squamous cell carcinoma (OSCC); however, this relationship has not been thoroughly characterized. Therefore, in this study, we compared the microbiota compositions between tumor sites and opposite normal tissues in buccal mucosal of 50 patients with OSCC using the 16S rDNA sequencing. Richness and diversity of bacteria were significantly higher in tumor sites than in the control tissues. Cancer tissues were enriched in six families (Prevotellaceae, Fusobacteriaceae, Flavobacteriaceae, Lachnospiraceae, Peptostreptococcaceae, and Campylobacteraceae) and 13 genera, including Fusobacterium, Alloprevotella and Porphyromonas. At the species level, the abundances of Fusobacterium nucleatum, Prevotella intermedia, Aggregatibacter segnis, Capnocytophaga leadbetteri, Peptostreptococcus stomatis, and another five species were significantly increased, suggesting a potential association between these bacteria and OSCC. Furthermore, the functional prediction revealed that genes involved in bacterial chemotaxis, flagellar assembly and lipopolysaccharide (LPS) biosynthesis which are associated with various pathological processes, were significantly increased in the OSCC group. Overall, oral bacterial profiles showed significant difference between cancer sites and normal tissue of OSCC patients, which might be onsidered diagnostic markers and treatment targets. Our study has been registered in the Chinese clinical trial registry (ChiCTR1900025253, http://www.chictr.org.cn/index.aspx).https://www.frontiersin.org/article/10.3389/fcimb.2019.00476/fulloral microbiotaoral squamous cell carcinomaFusobacterium nucleatumPrevotella intermediaPeptostreptococcus stomatis16S rDNA sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ling Zhang Ling Zhang Ling Zhang Yuan Liu Hua Jun Zheng Chen Ping Zhang |
spellingShingle |
Ling Zhang Ling Zhang Ling Zhang Yuan Liu Hua Jun Zheng Chen Ping Zhang The Oral Microbiota May Have Influence on Oral Cancer Frontiers in Cellular and Infection Microbiology oral microbiota oral squamous cell carcinoma Fusobacterium nucleatum Prevotella intermedia Peptostreptococcus stomatis 16S rDNA sequencing |
author_facet |
Ling Zhang Ling Zhang Ling Zhang Yuan Liu Hua Jun Zheng Chen Ping Zhang |
author_sort |
Ling Zhang |
title |
The Oral Microbiota May Have Influence on Oral Cancer |
title_short |
The Oral Microbiota May Have Influence on Oral Cancer |
title_full |
The Oral Microbiota May Have Influence on Oral Cancer |
title_fullStr |
The Oral Microbiota May Have Influence on Oral Cancer |
title_full_unstemmed |
The Oral Microbiota May Have Influence on Oral Cancer |
title_sort |
oral microbiota may have influence on oral cancer |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2020-01-01 |
description |
The oral microbiota plays an important role in the human microbiome and human health, and imbalances between microbes and their hosts can lead to oral and systemic diseases and chronic inflammation, which is usually caused by bacteria and contributes to cancer. There may be a relationship between oral bacteria and oral squamous cell carcinoma (OSCC); however, this relationship has not been thoroughly characterized. Therefore, in this study, we compared the microbiota compositions between tumor sites and opposite normal tissues in buccal mucosal of 50 patients with OSCC using the 16S rDNA sequencing. Richness and diversity of bacteria were significantly higher in tumor sites than in the control tissues. Cancer tissues were enriched in six families (Prevotellaceae, Fusobacteriaceae, Flavobacteriaceae, Lachnospiraceae, Peptostreptococcaceae, and Campylobacteraceae) and 13 genera, including Fusobacterium, Alloprevotella and Porphyromonas. At the species level, the abundances of Fusobacterium nucleatum, Prevotella intermedia, Aggregatibacter segnis, Capnocytophaga leadbetteri, Peptostreptococcus stomatis, and another five species were significantly increased, suggesting a potential association between these bacteria and OSCC. Furthermore, the functional prediction revealed that genes involved in bacterial chemotaxis, flagellar assembly and lipopolysaccharide (LPS) biosynthesis which are associated with various pathological processes, were significantly increased in the OSCC group. Overall, oral bacterial profiles showed significant difference between cancer sites and normal tissue of OSCC patients, which might be onsidered diagnostic markers and treatment targets. Our study has been registered in the Chinese clinical trial registry (ChiCTR1900025253, http://www.chictr.org.cn/index.aspx). |
topic |
oral microbiota oral squamous cell carcinoma Fusobacterium nucleatum Prevotella intermedia Peptostreptococcus stomatis 16S rDNA sequencing |
url |
https://www.frontiersin.org/article/10.3389/fcimb.2019.00476/full |
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