A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells.
Principal epididymal cells have one of the largest and more developed Golgi complex of mammalian cells. In the present study, we have used this cell as model for the study of the three-dimensional architecture of the Golgi complex of highly secretory and endocytic cells. Electron tomography demonstr...
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doaj-285f6037c4e146c7add9b2d01180b83c2020-11-25T02:29:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018555710.1371/journal.pone.0185557A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells.Narcisa Martínez-MartínezEmma Martínez-AlonsoMónica TomásJosef NeumüllerMargit PavelkaJosé A Martínez-MenárguezPrincipal epididymal cells have one of the largest and more developed Golgi complex of mammalian cells. In the present study, we have used this cell as model for the study of the three-dimensional architecture of the Golgi complex of highly secretory and endocytic cells. Electron tomography demonstrated the presence in this cell type of some unknown or very unusual Golgi structures such as branched cisternae, pocket-like cisternal invaginations or tubular connections. In addition, we have used this methodology and immunoelectron microscopy to analyze the close relationship between this organelle and both the endoplasmic reticulum and microtubules, and to describe in detail how these elements interact with compact and non-compact regions of the ribbon.http://europepmc.org/articles/PMC5619803?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Narcisa Martínez-Martínez Emma Martínez-Alonso Mónica Tomás Josef Neumüller Margit Pavelka José A Martínez-Menárguez |
spellingShingle |
Narcisa Martínez-Martínez Emma Martínez-Alonso Mónica Tomás Josef Neumüller Margit Pavelka José A Martínez-Menárguez A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells. PLoS ONE |
author_facet |
Narcisa Martínez-Martínez Emma Martínez-Alonso Mónica Tomás Josef Neumüller Margit Pavelka José A Martínez-Menárguez |
author_sort |
Narcisa Martínez-Martínez |
title |
A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells. |
title_short |
A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells. |
title_full |
A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells. |
title_fullStr |
A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells. |
title_full_unstemmed |
A new insight into the three-dimensional architecture of the Golgi complex: Characterization of unusual structures in epididymal principal cells. |
title_sort |
new insight into the three-dimensional architecture of the golgi complex: characterization of unusual structures in epididymal principal cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2017-01-01 |
description |
Principal epididymal cells have one of the largest and more developed Golgi complex of mammalian cells. In the present study, we have used this cell as model for the study of the three-dimensional architecture of the Golgi complex of highly secretory and endocytic cells. Electron tomography demonstrated the presence in this cell type of some unknown or very unusual Golgi structures such as branched cisternae, pocket-like cisternal invaginations or tubular connections. In addition, we have used this methodology and immunoelectron microscopy to analyze the close relationship between this organelle and both the endoplasmic reticulum and microtubules, and to describe in detail how these elements interact with compact and non-compact regions of the ribbon. |
url |
http://europepmc.org/articles/PMC5619803?pdf=render |
work_keys_str_mv |
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