Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers
Poerba YS, Ahmad F (2010) Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers. Biodiversitas 11: 118-123. This study was done to assess the molecular diversity of 36 accessions (18 cultivars) of the plantain and cooking bananas (Musa acuminata x M. balbis...
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Online Access: | http://biodiversitas.mipa.uns.ac.id/D/D1103/D110303.pdf |
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doaj-2886e94f01b14e96937aa85fc2c5492a2020-11-24T22:41:39ZengMBI & UNS SoloBiodiversitas1412-033X2085-47222010-07-01113118123Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers YUYU SURYASARI POERBAFAJARUDIN AHMADPoerba YS, Ahmad F (2010) Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers. Biodiversitas 11: 118-123. This study was done to assess the molecular diversity of 36 accessions (18 cultivars) of the plantain and cooking bananas (Musa acuminata x M. balbisiana, AAB, ABB subgroups) based on Random amplified polymorphic DNA (RAPD) and and Inter Simple Sequence Repeats (ISSR) markers and to determine genetic relationships in the bananas. RAPD and ISSR fingerprinting of these banana varieties was carried out by five primers of RAPDs and two primers of ISSRs. RAPD primers produced 63 amplified fragments varying from 250 to 2500 bp in size. 96.82% of the amplification bands were polymorphic. ISSR primers produced 26 amplified fragments varying from 350 bp to 2000 bp in size. The results showed that 92.86% of the amplification bands were polymorphic. The range of genetic distance of 18 cultivars was from 0.06-0.67. http://biodiversitas.mipa.uns.ac.id/D/D1103/D110303.pdfRAPD analysisMusa acuminataMusa balbisianaplantaincooking bananas. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
YUYU SURYASARI POERBA FAJARUDIN AHMAD |
spellingShingle |
YUYU SURYASARI POERBA FAJARUDIN AHMAD Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers Biodiversitas RAPD analysis Musa acuminata Musa balbisiana plantain cooking bananas. |
author_facet |
YUYU SURYASARI POERBA FAJARUDIN AHMAD |
author_sort |
YUYU SURYASARI POERBA |
title |
Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers |
title_short |
Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers |
title_full |
Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers |
title_fullStr |
Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers |
title_full_unstemmed |
Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers |
title_sort |
genetic variability among 18 cultivars of cooking bananas and plantains by rapd and issr markers |
publisher |
MBI & UNS Solo |
series |
Biodiversitas |
issn |
1412-033X 2085-4722 |
publishDate |
2010-07-01 |
description |
Poerba YS, Ahmad F (2010) Genetic variability among 18 cultivars of cooking bananas and plantains by RAPD and ISSR markers. Biodiversitas 11: 118-123. This study was done to assess the molecular diversity of 36 accessions (18 cultivars) of the plantain and cooking bananas (Musa acuminata x M. balbisiana, AAB, ABB subgroups) based on Random amplified polymorphic DNA (RAPD) and and Inter Simple Sequence Repeats (ISSR) markers and to determine genetic relationships in the bananas. RAPD and ISSR fingerprinting of these banana varieties was carried out by five primers of RAPDs and two primers of ISSRs. RAPD primers produced 63 amplified fragments varying from 250 to 2500 bp in size. 96.82% of the amplification bands were polymorphic. ISSR primers produced 26 amplified fragments varying from 350 bp to 2000 bp in size. The results showed that 92.86% of the amplification bands were polymorphic. The range of genetic distance of 18 cultivars was from 0.06-0.67. |
topic |
RAPD analysis Musa acuminata Musa balbisiana plantain cooking bananas. |
url |
http://biodiversitas.mipa.uns.ac.id/D/D1103/D110303.pdf |
work_keys_str_mv |
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