Anti-Fatigue and Antioxidant Activity of the Polysaccharides Isolated from Millettiae speciosae Champ. Leguminosae
Millettiae speciosae Champ. Leguminosae (MSC), is a well-known Chinese herb traditionally used as food material and medicine for enhancing physical strength. Our preliminary study found that the aqueous extract of this herb (MSE) had an anti-fatigue effect. In this paper, we further separated MSE in...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2015-10-01
|
Series: | Nutrients |
Subjects: | |
Online Access: | http://www.mdpi.com/2072-6643/7/10/5422 |
id |
doaj-2895802bad5848c98b3b7754dd266c50 |
---|---|
record_format |
Article |
spelling |
doaj-2895802bad5848c98b3b7754dd266c502020-11-24T22:12:50ZengMDPI AGNutrients2072-66432015-10-017108657866910.3390/nu7105422nu7105422Anti-Fatigue and Antioxidant Activity of the Polysaccharides Isolated from Millettiae speciosae Champ. LeguminosaeXiao-Ning Zhao0Jia-Li Liang1Han-Bin Chen2Ye-Er Liang3Hui-Zhen Guo4Ze-Ren Su5Yu-Cui Li6Hui-Fang Zeng7Xiao-Jun Zhang8The First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaThe First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaThe First Affiliated Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510405, ChinaSchool of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaMillettiae speciosae Champ. Leguminosae (MSC), is a well-known Chinese herb traditionally used as food material and medicine for enhancing physical strength. Our preliminary study found that the aqueous extract of this herb (MSE) had an anti-fatigue effect. In this paper, we further separated MSE into total polysaccharides (MSP) and supernatant (MSS) by alcohol precipitation, and explored which fraction was active for its anti-fatigue effect. Mice were orally administered with MSP or MSS at the doses of 200, 400, and 800 mg/kg for 20 days and the anti-fatigue effect was assessed by exhaustive swimming exercise (ESE). The biochemical parameters related to fatigue after ESE and the in vitro antioxidant activity of active fraction were determined. Our results showed that MSP, instead of MSS, significantly extended the swimming time to exhaustion (p < 0.05), indicating that MSP is responsible for the anti-fatigue effect of MSE. In addition, MSP treatment increased the levels of glucose (Glu) and muscle glycogen, whereas it decreased the accumulations of blood urea nitrogen (BUN) and lactic acid (Lac). Moreover, ESE increased the levels of creatine phosphokinase (CK), lactic dehydrogenase (LDH), and malondialdehyde (MDA) but reduced superoxide dismutase (SOD) and glutathione (GSH) in plasma. In contrast, MSP inhibited all the above changes relating to fatigue. Furthermore, an in vitro antioxidant test revealed that MSP dose-dependently scavenged ·OH and DPPH free radicals. Taken together, these findings strongly suggested that MSP was able to alleviate physical fatigue by increasing energy resources and decreasing accumulation of detrimental metabolites. The antioxidant activity may crucially contribute to the observed anti-fatigue effect of MSP.http://www.mdpi.com/2072-6643/7/10/5422Millettiae speciosae Champ. polysaccharides anti-fatigue activityantioxidant activityphysical performancebiochemical indicators |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiao-Ning Zhao Jia-Li Liang Han-Bin Chen Ye-Er Liang Hui-Zhen Guo Ze-Ren Su Yu-Cui Li Hui-Fang Zeng Xiao-Jun Zhang |
spellingShingle |
Xiao-Ning Zhao Jia-Li Liang Han-Bin Chen Ye-Er Liang Hui-Zhen Guo Ze-Ren Su Yu-Cui Li Hui-Fang Zeng Xiao-Jun Zhang Anti-Fatigue and Antioxidant Activity of the Polysaccharides Isolated from Millettiae speciosae Champ. Leguminosae Nutrients Millettiae speciosae Champ. polysaccharides anti-fatigue activity antioxidant activity physical performance biochemical indicators |
author_facet |
Xiao-Ning Zhao Jia-Li Liang Han-Bin Chen Ye-Er Liang Hui-Zhen Guo Ze-Ren Su Yu-Cui Li Hui-Fang Zeng Xiao-Jun Zhang |
author_sort |
Xiao-Ning Zhao |
title |
Anti-Fatigue and Antioxidant Activity of the Polysaccharides Isolated from Millettiae speciosae Champ. Leguminosae |
title_short |
Anti-Fatigue and Antioxidant Activity of the Polysaccharides Isolated from Millettiae speciosae Champ. Leguminosae |
title_full |
Anti-Fatigue and Antioxidant Activity of the Polysaccharides Isolated from Millettiae speciosae Champ. Leguminosae |
title_fullStr |
Anti-Fatigue and Antioxidant Activity of the Polysaccharides Isolated from Millettiae speciosae Champ. Leguminosae |
title_full_unstemmed |
Anti-Fatigue and Antioxidant Activity of the Polysaccharides Isolated from Millettiae speciosae Champ. Leguminosae |
title_sort |
anti-fatigue and antioxidant activity of the polysaccharides isolated from millettiae speciosae champ. leguminosae |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2015-10-01 |
description |
Millettiae speciosae Champ. Leguminosae (MSC), is a well-known Chinese herb traditionally used as food material and medicine for enhancing physical strength. Our preliminary study found that the aqueous extract of this herb (MSE) had an anti-fatigue effect. In this paper, we further separated MSE into total polysaccharides (MSP) and supernatant (MSS) by alcohol precipitation, and explored which fraction was active for its anti-fatigue effect. Mice were orally administered with MSP or MSS at the doses of 200, 400, and 800 mg/kg for 20 days and the anti-fatigue effect was assessed by exhaustive swimming exercise (ESE). The biochemical parameters related to fatigue after ESE and the in vitro antioxidant activity of active fraction were determined. Our results showed that MSP, instead of MSS, significantly extended the swimming time to exhaustion (p < 0.05), indicating that MSP is responsible for the anti-fatigue effect of MSE. In addition, MSP treatment increased the levels of glucose (Glu) and muscle glycogen, whereas it decreased the accumulations of blood urea nitrogen (BUN) and lactic acid (Lac). Moreover, ESE increased the levels of creatine phosphokinase (CK), lactic dehydrogenase (LDH), and malondialdehyde (MDA) but reduced superoxide dismutase (SOD) and glutathione (GSH) in plasma. In contrast, MSP inhibited all the above changes relating to fatigue. Furthermore, an in vitro antioxidant test revealed that MSP dose-dependently scavenged ·OH and DPPH free radicals. Taken together, these findings strongly suggested that MSP was able to alleviate physical fatigue by increasing energy resources and decreasing accumulation of detrimental metabolites. The antioxidant activity may crucially contribute to the observed anti-fatigue effect of MSP. |
topic |
Millettiae speciosae Champ. polysaccharides anti-fatigue activity antioxidant activity physical performance biochemical indicators |
url |
http://www.mdpi.com/2072-6643/7/10/5422 |
work_keys_str_mv |
AT xiaoningzhao antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae AT jialiliang antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae AT hanbinchen antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae AT yeerliang antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae AT huizhenguo antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae AT zerensu antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae AT yucuili antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae AT huifangzeng antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae AT xiaojunzhang antifatigueandantioxidantactivityofthepolysaccharidesisolatedfrommillettiaespeciosaechampleguminosae |
_version_ |
1725802187342217216 |