Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41

Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41

To elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS-41, the -carotene desaturase gene (zds) was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends. The full-length cDNA sequence was 2031 bp and contained 17...

Full description

Bibliographic Details
Main Authors: Meiya Li, Zhibing Gan, Yan Cui, Chunlei Shi, Xianming Shi
Format: Article
Language:English
Published: Hindawi Limited 2011-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2011/731542
id doaj-289ce4ee716e4037b8fa03582f0d7c2b
record_format Article
spelling doaj-289ce4ee716e4037b8fa03582f0d7c2b2020-11-25T00:48:42ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512011-01-01201110.1155/2011/731542731542Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41Meiya Li0Zhibing Gan1Yan Cui2Chunlei Shi3Xianming Shi4MOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center, Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center, Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center, Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center, Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety and Bor Luh Food Safety Center, Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaTo elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS-41, the -carotene desaturase gene (zds) was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends. The full-length cDNA sequence was 2031 bp and contained 1755 bp putative open reading frame which encodes a 584 amino acid deduced polypeptide whose computed molecular weight was 63.7 kDa. Sequence homology research indicated that the nucleotide and putative protein had sequence identities of 72.5% and 69.5% with those of the green alga Chlamydomonas reinhardtii, respectively. Phylogenetic analysis demonstrated that the ZDS from C. protothecoides CS-41 had a closer relationship with those of chlorophyta and higher plants than with those of other species. In addition, we also found that the zds gene expression was upregulated in response to light.http://dx.doi.org/10.1155/2011/731542
collection DOAJ
language English
format Article
sources DOAJ
author Meiya Li
Zhibing Gan
Yan Cui
Chunlei Shi
Xianming Shi
spellingShingle Meiya Li
Zhibing Gan
Yan Cui
Chunlei Shi
Xianming Shi
Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41
Journal of Biomedicine and Biotechnology
author_facet Meiya Li
Zhibing Gan
Yan Cui
Chunlei Shi
Xianming Shi
author_sort Meiya Li
title Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41
title_short Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41
title_full Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41
title_fullStr Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41
title_full_unstemmed Cloning and Characterization of the -Carotene Desaturase Gene from Chlorella protothecoides CS-41
title_sort cloning and characterization of the -carotene desaturase gene from chlorella protothecoides cs-41
publisher Hindawi Limited
series Journal of Biomedicine and Biotechnology
issn 1110-7243
1110-7251
publishDate 2011-01-01
description To elucidate the lutein biosynthesis pathway in the lutein-producing alga, Chlorella protothecoides CS-41, the -carotene desaturase gene (zds) was isolated from Chlorella protothecoides using the approach of rapid amplification of cDNA ends. The full-length cDNA sequence was 2031 bp and contained 1755 bp putative open reading frame which encodes a 584 amino acid deduced polypeptide whose computed molecular weight was 63.7 kDa. Sequence homology research indicated that the nucleotide and putative protein had sequence identities of 72.5% and 69.5% with those of the green alga Chlamydomonas reinhardtii, respectively. Phylogenetic analysis demonstrated that the ZDS from C. protothecoides CS-41 had a closer relationship with those of chlorophyta and higher plants than with those of other species. In addition, we also found that the zds gene expression was upregulated in response to light.
url http://dx.doi.org/10.1155/2011/731542
work_keys_str_mv AT meiyali cloningandcharacterizationofthecarotenedesaturasegenefromchlorellaprotothecoidescs41
AT zhibinggan cloningandcharacterizationofthecarotenedesaturasegenefromchlorellaprotothecoidescs41
AT yancui cloningandcharacterizationofthecarotenedesaturasegenefromchlorellaprotothecoidescs41
AT chunleishi cloningandcharacterizationofthecarotenedesaturasegenefromchlorellaprotothecoidescs41
AT xianmingshi cloningandcharacterizationofthecarotenedesaturasegenefromchlorellaprotothecoidescs41
_version_ 1725254865720967168

Similar Items