Development and Validation of an Effective CRISPR/Cas9 Vector for Efficiently Isolating Positive Transformants and Transgene-Free Mutants in a Wide Range of Plant Species

Development and Validation of an Effective CRISPR/Cas9 Vector for Efficiently Isolating Positive Transformants and Transgene-Free Mutants in a Wide Range of Plant Species

The CRISPR/Cas9 technique is a highly valuable tool in creating new materials for both basic and applied researches. Previously, we succeeded in effectively generating mutations in Brassica napus using an available CRISPR/Cas9 vector pKSE401, while isolation of Cas9-free mutants is laborious and ine...

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Bibliographic Details
Main Authors: Ting Tang, Xiwen Yu, Hong Yang, Qi Gao, Hongtao Ji, Yanxu Wang, Guanbo Yan, Yan Peng, Huifeng Luo, Kede Liu, Xia Li, Chaozhi Ma, Chunying Kang, Cheng Dai
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-10-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
visual screening
genome editing
Arabidopsis
B. napus
strawberry
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2018.01533/full

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