Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate
Lijuan Xu,1,* Ping Wang,2,* Jing Cheng,2 Shangshang Qin,2,3 Weihong Xie1 1The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450001, China; 2School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China; 3Key Laboratory of Advanced Drug Preparation Tech...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Dove Medical Press
2019-03-01
|
Series: | Infection and Drug Resistance |
Subjects: | |
Online Access: | https://www.dovepress.com/characterization-of-a-novel-blandm-5-harboring-incfii-plasmid-and-an-m-peer-reviewed-article-IDR |
id |
doaj-28e0a85efa244b2e8a14f6cb12045a20 |
---|---|
record_format |
Article |
spelling |
doaj-28e0a85efa244b2e8a14f6cb12045a202020-11-24T21:15:57ZengDove Medical PressInfection and Drug Resistance1178-69732019-03-01Volume 1251151944389Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolateXu LWang PCheng JQin SXie WLijuan Xu,1,* Ping Wang,2,* Jing Cheng,2 Shangshang Qin,2,3 Weihong Xie1 1The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450001, China; 2School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China; 3Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, Henan 450001, China *These authors contributed equally to this work Background and purpose: The spread of the plasmid-mediated, colistin-resistance gene mcr-1 into New Delhi metallo-β-lactamase (NDM)-producing bacterial isolates can cause untreatable infections. In this study, we conducted a molecular characterization of a novel, conjugative, bla NDM-5-positive IncFII plasmid (pNDM-EC16-50) together with an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate EC16-50.Methods and results: EC16-50, which belongs to the E. coli strain ST167 and phylogroup A, was identified to co-produce NDM-5 and MCR-1. S1-PFGE and Southern blotting showed that blaNDM-5 and mcr-1 genes were located on ~95 kb and ~65 kb plasmids, respectively. A conjugation assay revealed that both blaNDM-5- and mcr-1-bearing plasmids were self-transmissible. Comparative plasmid analysis suggested that blaNDM-5-harboring F2:A-:B-plasmid might have evolved from the well-reported NDM-carrying pMC-NDM-like plasmid via recombination with a locally emerged pSJ_94 plasmid, whereas the mcr-1-carrying IncI2 plasmid was similar to previously reported mcr-1-bearing plasmids in China.Conclusion and impact: This study represents the first report of the NDM-5 carrying IncFII- but not IncX3-type plasmid in an MCR-1-producing E. coli isolate. More striking was the dissemination of mcr-1 in a successful epidemic NDM-5-producing E. coli clone ST167, which could facilitate the spread of colistin resistance in carbapenemase-producing E. coli isolates. Keywords: Escherichia coli, ST167, MCR-1, IncFII plasmid, NDM-5 https://www.dovepress.com/characterization-of-a-novel-blandm-5-harboring-incfii-plasmid-and-an-m-peer-reviewed-article-IDREscherichia coli ST167MCR-1IncFII plasmidNDM-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xu L Wang P Cheng J Qin S Xie W |
spellingShingle |
Xu L Wang P Cheng J Qin S Xie W Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate Infection and Drug Resistance Escherichia coli ST167 MCR-1 IncFII plasmid NDM-5 |
author_facet |
Xu L Wang P Cheng J Qin S Xie W |
author_sort |
Xu L |
title |
Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate |
title_short |
Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate |
title_full |
Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate |
title_fullStr |
Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate |
title_full_unstemmed |
Characterization of a novel blaNDM-5-harboring IncFII plasmid and an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate |
title_sort |
characterization of a novel blandm-5-harboring incfii plasmid and an mcr-1-bearing inci2 plasmid in a single escherichia coli st167 clinical isolate |
publisher |
Dove Medical Press |
series |
Infection and Drug Resistance |
issn |
1178-6973 |
publishDate |
2019-03-01 |
description |
Lijuan Xu,1,* Ping Wang,2,* Jing Cheng,2 Shangshang Qin,2,3 Weihong Xie1 1The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450001, China; 2School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China; 3Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, Zhengzhou University, Zhengzhou, Henan 450001, China *These authors contributed equally to this work Background and purpose: The spread of the plasmid-mediated, colistin-resistance gene mcr-1 into New Delhi metallo-β-lactamase (NDM)-producing bacterial isolates can cause untreatable infections. In this study, we conducted a molecular characterization of a novel, conjugative, bla NDM-5-positive IncFII plasmid (pNDM-EC16-50) together with an mcr-1-bearing IncI2 plasmid in a single Escherichia coli ST167 clinical isolate EC16-50.Methods and results: EC16-50, which belongs to the E. coli strain ST167 and phylogroup A, was identified to co-produce NDM-5 and MCR-1. S1-PFGE and Southern blotting showed that blaNDM-5 and mcr-1 genes were located on ~95 kb and ~65 kb plasmids, respectively. A conjugation assay revealed that both blaNDM-5- and mcr-1-bearing plasmids were self-transmissible. Comparative plasmid analysis suggested that blaNDM-5-harboring F2:A-:B-plasmid might have evolved from the well-reported NDM-carrying pMC-NDM-like plasmid via recombination with a locally emerged pSJ_94 plasmid, whereas the mcr-1-carrying IncI2 plasmid was similar to previously reported mcr-1-bearing plasmids in China.Conclusion and impact: This study represents the first report of the NDM-5 carrying IncFII- but not IncX3-type plasmid in an MCR-1-producing E. coli isolate. More striking was the dissemination of mcr-1 in a successful epidemic NDM-5-producing E. coli clone ST167, which could facilitate the spread of colistin resistance in carbapenemase-producing E. coli isolates. Keywords: Escherichia coli, ST167, MCR-1, IncFII plasmid, NDM-5 |
topic |
Escherichia coli ST167 MCR-1 IncFII plasmid NDM-5 |
url |
https://www.dovepress.com/characterization-of-a-novel-blandm-5-harboring-incfii-plasmid-and-an-m-peer-reviewed-article-IDR |
work_keys_str_mv |
AT xul characterizationofanovelblandm5harboringincfiiplasmidandanmcr1bearinginci2plasmidinasingleescherichiacolist167clinicalisolate AT wangp characterizationofanovelblandm5harboringincfiiplasmidandanmcr1bearinginci2plasmidinasingleescherichiacolist167clinicalisolate AT chengj characterizationofanovelblandm5harboringincfiiplasmidandanmcr1bearinginci2plasmidinasingleescherichiacolist167clinicalisolate AT qins characterizationofanovelblandm5harboringincfiiplasmidandanmcr1bearinginci2plasmidinasingleescherichiacolist167clinicalisolate AT xiew characterizationofanovelblandm5harboringincfiiplasmidandanmcr1bearinginci2plasmidinasingleescherichiacolist167clinicalisolate |
_version_ |
1716743978146070528 |