Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01
Chromium(III) is an essential trace element for humans and animals. Pleutorus cornucopiae SD-01 is a nutritional and functional mushroom containing many kinds of bioactive ingredients. The aims of this work are to optimize the conditions of P. cornucopiae SD-01 cultivation with Cr enrichment in subm...
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doaj-28e8f99cc82d456594739ca7f4e9f7612020-11-25T02:35:55ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062010-01-01481107113Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01Fan-Yun Meng0Xiao-Nan Liu1Zhen Song2Peng Deng3Ke-Ming Fan4Le Jia5College of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR ChinaCollege of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR ChinaCollege of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR ChinaCollege of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR ChinaCollege of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR ChinaCollege of Life Sciences, Shandong Agricultural University, Taian, Shandong, CN-271018, PR ChinaChromium(III) is an essential trace element for humans and animals. Pleutorus cornucopiae SD-01 is a nutritional and functional mushroom containing many kinds of bioactive ingredients. The aims of this work are to optimize the conditions of P. cornucopiae SD-01 cultivation with Cr enrichment in submerged culture by determining the dry cell mass, Cr content in mycelia and the rate of Cr enrichment, and to analyze the amino acid composition of Cr-enriched mycelia. The optimal medium contained (in g/L): potato 200, sucrose 25, yeast extract 4, KH2PO4 1 and MgSO4·7H2O 1. The optimum parameters of liquid culture were temperature 25 °C, cultivation time 6 days, the volume of the medium 100 mL, rotation speed 160 rpm and initial pH=6.5. Under the optimized conditions, the values of the dry cell mass, Cr content in mycelia and the rate of Cr enrichment were (6.63±0.35) g/L, (3670±211) μg/g and (12.15±1.01) % respectively, which were by (23.23±1.22), (18.19±1.06) and (45.68±2.67) % respectively, higher than those in the control. Chromium(III) in Cr-enriched mycelia was mainly combined with protein and polysaccharide. The contents of total amino acids and essential amino acids in Cr-enriched mycelia were increased by (31.25±0.58) and (44.26±0.76) %, respectively.http://hrcak.srce.hr/file/74766chromium(III)Cr-enrichmentPleutorus cornucopiae SD-01culture optimizationamino acid composition |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fan-Yun Meng Xiao-Nan Liu Zhen Song Peng Deng Ke-Ming Fan Le Jia |
spellingShingle |
Fan-Yun Meng Xiao-Nan Liu Zhen Song Peng Deng Ke-Ming Fan Le Jia Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01 Food Technology and Biotechnology chromium(III) Cr-enrichment Pleutorus cornucopiae SD-01 culture optimization amino acid composition |
author_facet |
Fan-Yun Meng Xiao-Nan Liu Zhen Song Peng Deng Ke-Ming Fan Le Jia |
author_sort |
Fan-Yun Meng |
title |
Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01 |
title_short |
Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01 |
title_full |
Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01 |
title_fullStr |
Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01 |
title_full_unstemmed |
Culture Optimization and Amino Acid Composition of Cr-Enriched Mycelia of Pleurotus cornucopiae SD-01 |
title_sort |
culture optimization and amino acid composition of cr-enriched mycelia of pleurotus cornucopiae sd-01 |
publisher |
University of Zagreb |
series |
Food Technology and Biotechnology |
issn |
1330-9862 1334-2606 |
publishDate |
2010-01-01 |
description |
Chromium(III) is an essential trace element for humans and animals. Pleutorus cornucopiae SD-01 is a nutritional and functional mushroom containing many kinds of bioactive ingredients. The aims of this work are to optimize the conditions of P. cornucopiae SD-01 cultivation with Cr enrichment in submerged culture by determining the dry cell mass, Cr content in mycelia and the rate of Cr enrichment, and to analyze the amino acid composition of Cr-enriched mycelia. The optimal medium contained (in g/L): potato 200, sucrose 25, yeast extract 4, KH2PO4 1 and MgSO4·7H2O 1. The optimum parameters of liquid culture were temperature 25 °C, cultivation time 6 days, the volume of the medium 100 mL, rotation speed 160 rpm and initial pH=6.5. Under the optimized conditions, the values of the dry cell mass, Cr content in mycelia and the rate of Cr enrichment were (6.63±0.35) g/L, (3670±211) μg/g and (12.15±1.01) % respectively, which were by (23.23±1.22), (18.19±1.06) and (45.68±2.67) % respectively, higher than those in the control. Chromium(III) in Cr-enriched mycelia was mainly combined with protein and polysaccharide. The contents of total amino acids and essential amino acids in Cr-enriched mycelia were increased by (31.25±0.58) and (44.26±0.76) %, respectively. |
topic |
chromium(III) Cr-enrichment Pleutorus cornucopiae SD-01 culture optimization amino acid composition |
url |
http://hrcak.srce.hr/file/74766 |
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